Therapeutic monoclonal antibodies have the potential to cause an unwanted immune response, resulting in anti-drug antibody production (fennel). Fennel binding to drugs and subsequent formation of the immune complex (ICS) can trigger various responses, depending on the size, concentration, and subclass of Agas. To better understand the impact of Agas on pharmacokinetics, pharmacodynamics, and toxicological profiles, the bioanalysis method was developed to detect ICS between the Immunoglobulin G (IGG) of humans and Agas in biological samples.
Regarding experimental procedures, briefly, the specific ICS of human antibodies and human antibodies that are not bound in biological samples are separated through electrophoresis of original blue polyacrylamide gel (BN-PA-Page). The target fraction is then cut from the gel, followed by the digestion of Trypsin in the gel and liquid chromatography then the spectrometry of the spectrometry (LC-MS / MS) to monitor human specific peptides. This method is able to detect various types of human antibodies with lower detection limits than 10 μg / ml in serum monkeys. The performance of testing for ICS detection is shown using barbed samples, and pre-incubation ICs in the monkey serum are clearly detected.
Taken together, these findings indicate that our method allows semi quantitative analysis to estimate the ratio of human antibodies including IC compared to total antibodies. This method was successfully applied to the study in Vivo using mice, and data helped explain unexpectedly cleaning antibodies due to large IC formation. The combination of ICS separation by BN-PAGE and Human IgG specific peptide detection by LC-MS / MS is a common bioanalytical approach that is useful for Detection of ICS in animals.
The characterization of the eubacterial community by denying the gradient gel electrophoresis (DGGE) and the next generation sequencing (NGS) in the biotrickling deculfurization filter using progressive changes in nitrate and nitrite as the final electron acceptor.
Anoxic biotrickling filters (BTFS) represent the technology with high H2S elimination capacity and removal efficiency which is widely studied for biogas desulfurization. Three changes in the final electron acceptor are made using nitrate and nitrite during the operating period of 520 days. BTF’s anoxic stability and performance is maintained when a significant disruption is applied to a system involving progressive changes in nitrate to nitrite and vice versa.
Here the impact of changes in electron acceptors in the microbial community are characterized by denaturation of electrophoresis (DGGE) gradient gel and the next generation (NGS) sequencing. Both platforms revealed that the community underwent changes during perturbations but was tough because the transfer capacity did not significantly change. Proteobacteria and bacteroides are the main phylum and sulfurimonas and Tiobacillus which are the oxidizing genera-oxidizing-oxidizing (NR-SOB) involved in the biodesulfurization process
Monitoring the plasmid vaccine topology with capillary gel electrophoresis.
Plasmid DNA has been widely used in vaccination as well as in cells and gen therapy. It exists in some isoforms including supercoiled, scelled or open circular and linear forms. Regulatory institutions recommend having more than 80% of supercoiled isoforms for mass release plasmid products; Thus must be analyzed accordingly. The traditional analysis method for Plasmid DNA is Gel Gel Electrophoresis. However, because manual sample loading, visualization, and analysis of manual data, it has limitations in obtaining consistent quantitative results.

In this brief communication, we introduce a fast, sensitive, and powerful plasmid analysis method using capillary electrophoresis with fluorescence detection of laserinduced (CE-LIF). CE-LIF analysis of the isoform Supercoiled and open circle partners are finished in 20 minutes with excellent sensitivity by using the dye binding the same neon flow. The advantage of this method is indicated by the purity analysis of two large plasmids (7 KB and 10 KB). Sample loading, separation, and completely automatic data analysis display repetition of improved tests and ease of quantization of gel electrophoresis gel agarose degradation.
Molecular typing acinetobacter baumannii which resistant drugs from clinical and environmental specimens in three Iranian hospitals with pulsed field gel electrophoresis.
Multi-Medicine Resistant (MDR) Acinetobacter Baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and clinical relations of clinical and environmental isolates A. Baumannii with the electrophoresis method of field gel pulsed. Forty-three clinical and 26 environmental isolates from MDR A. Baumannii was collected and recognized through 20ne fire. Antibiotic resistance of isolates is assessed by disk diffusion method, and biofilm formation tests are carried out by the microtiter plate method. Field gel electrophoresis (PFGE) is used to assess the genome features of bacterial isolates.
The resistance level of clinical and environmental isolates to antibiotics comes from 95 to 100%. The difference between antibiotic resistance between clinical and environmental isolates is not statistically significant (p> 0.05). The ability of biofilm production reveals that 31 (44.9%), and 30 (43.5%) isolates have strong and moderate biofilm producers activities. Typing PFGGes shows eight different clusters (A, B, C, D, E, F, G, and H) with two significant clusters including A and G with 21 (30.4%) and 16 (23.2%) each -Masing, which consists of 53.6% of all isolates. There is no relationship between biofilm formation and antibiotic resistance patterns with pulsotypes of PFGE.
CometAssay Single Cell Gel Electrophoresis Assay-Silver |
4251-050-K |
Biotechne |
1 kit |
EUR 684.96 |
Agarose gel DNA extraction and purification kit |
K1441-100 |
Biovision |
each |
EUR 510 |
Agarose Gel DNA Extraction and Purification |
EP10010 |
Neuromics |
100 Tests |
EUR 356.4 |
ES 6 Tooth Gel Comb For ES Electrophoresis |
LE1011-A4 |
GenDepot |
Ea |
EUR 148.8 |
ES 8/10 Double Gel Comb For ES Electrophoresis |
LE1011-A3 |
GenDepot |
Ea |
EUR 148.8 |
Cys-link agarose gel coupling buffer kit (5-10 peptides) |
110400-CB |
Alpha Diagnostics |
1 kit |
EUR 270 |
CometAssay Electrophoresis Starter Kit |
4250-050-ESK |
Biotechne |
1 Kit |
EUR 3749.28 |
Gel-FAST? Gel Staining/Destaining Kit |
K901-40 |
Biovision |
each |
EUR 210 |
CometAssay Electrophoresis System |
4250-050-ES |
Biotechne |
1 Kit |
EUR 3540.96 |
electrophoresis power supply |
EV245 |
Consort |
ea |
EUR 574.8 |
Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins) |
110100-CG |
Alpha Diagnostics |
5 ml |
EUR 343.2 |
Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins) |
110200-NG |
Alpha Diagnostics |
5 ml |
EUR 343.2 |
E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies |
EC11-G |
Alpha Diagnostics |
1 ml |
EUR 343.2 |
Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies |
KLH11-G |
Alpha Diagnostics |
1 ml |
EUR 270 |
Agarose |
abx082006-100g |
Abbexa |
100 g |
EUR 292.8 |
|
Agarose |
abx082007-100g |
Abbexa |
100 g |
EUR 309.6 |
|
Agarose |
abx082008-100g |
Abbexa |
100 g |
EUR 276 |
|
Agarose |
G060-1 |
ABM |
100 g |
EUR 211.2 |
Agarose |
G060-2 |
ABM |
500 g |
EUR 442.8 |
Agarose |
Z5050007 |
Biochain |
500 g |
EUR 738 |
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
Agarose |
Z5050013 |
Biochain |
100 g |
EUR 226.8 |
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
Maximo-Gel juice fluorescent Protein-Gel Gel stain |
310012 |
GeneOn |
10 ml |
EUR 222 |
Maximo-Gel juice fluorescent Protein-Gel Gel stain |
310012-L |
GeneOn |
10x10ml |
EUR 1904.4 |
Gel-Bright LED Gel Illuminator |
E90003 |
Biotium |
1EA |
EUR 927.6 |
Description: Minimum order quantity: 1 unit of 1EA |
myGel Mini Electrophoresis System |
BM0286 |
GenDepot |
System |
EUR 741.6 |
6X6 well Electrophoresis Apparatus |
EDK626 |
Bio Basic |
1 UNIT |
EUR 750.6 |
|
ES Horizontal Electrophoresis Set |
LE1011 |
GenDepot |
Set |
EUR 578.4 |
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380 |
ACT-CBMN-7 |
ACTGene |
each |
EUR 892.8 |
Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies |
KLH11-G-5 |
Alpha Diagnostics |
5 ml |
EUR 634.8 |
Precast Agarose Gel TAE, 2%, 12 Well, EtBr Stained, 20 gels per Box |
TAE12-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TAE, 2%, 17 Well, EtBr Stained, 20 gels per Box |
TAE17-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TAE, 2%, 48 Well, EtBr Stained, 10 gels per Box |
TAE48-2PER-EB |
Bio Basic |
1BOX, 10UNIT |
EUR 331.44 |
|
Precast Agarose Gel TBE, 2%, 12 Well, EtBr Stained, 20 gels per Box |
TBE12-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TBE, 2%, 17 Well, EtBr Stained, 20 gels per Box |
TBE17-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TBE, 2%, 48 Well, EtBr Stained, 10 gels per Box |
TBE48-2PER-EB |
Bio Basic |
1BOX, 10UNIT |
EUR 331.44 |
|
Quick Gel Extraction Kit |
20-abx098085 |
Abbexa |
|
|
|
Gel DNA Recovery Kit |
GP05 |
GeneOn |
5 preps |
Ask for price |
Gel DNA Recovery Kit |
GP100 |
GeneOn |
100 preps |
EUR 156 |
Gel DNA Recovery Kit |
GP200 |
GeneOn |
200 preps |
EUR 226.8 |
Gel DNA Recovery Kit |
GP50 |
GeneOn |
50 preps |
EUR 112.8 |
AmbiClean Kit (PCR & Gel) |
GV05 |
GeneOn |
5 preps |
Ask for price |
AmbiClean Kit (PCR & Gel) |
GV100 |
GeneOn |
100 preps |
EUR 153.6 |
AmbiClean Kit (PCR & Gel) |
GV200 |
GeneOn |
200 preps |
EUR 226.8 |
AmbiClean Kit (PCR & Gel) |
GV50 |
GeneOn |
50 preps |
EUR 109.2 |
Agarose, Sepro |
A0224-010 |
GenDepot |
100g |
EUR 262.8 |
Agarose, Sepro |
A0224-020 |
GenDepot |
200g |
EUR 343.2 |
Agarose, Sepro |
A0224-050 |
GenDepot |
500g |
EUR 522 |
Agarose L.M.P |
20-abx082386 |
Abbexa |
|
|
|
Agarose LE |
A-1270-100 |
ABTBeads |
100 g |
EUR 139.2 |
Agarose LE |
A-1270-500 |
ABTBeads |
500 g |
EUR 349.2 |
Agarose HR |
A-1280-100 |
ABTBeads |
100 g |
EUR 219.6 |
Agarose HR |
A-1280-500 |
ABTBeads |
500 g |
EUR 822 |
Agarose GA |
A-1290-100 |
ABTBeads |
100 g |
EUR 123.6 |
Agarose GA |
A-1290-500 |
ABTBeads |
500 g |
EUR 306 |
Agarose LM |
A-1300-100 |
ABTBeads |
100 g |
EUR 230.4 |
Agarose LM |
A-1300-25 |
ABTBeads |
25 g |
EUR 123.6 |
Agarose Tablets |
BA50201 |
Bioatlas |
100pcs |
EUR 130.8 |
Description: Ultrapure agarose for separation and further analysis of PCR products. |
Agarose StandardRoomTemperature |
FYA002-100G |
Yeastern Biotech |
100 g |
Ask for price |
Agarose StandardRoomTemperature |
FYA002-500G |
Yeastern Biotech |
500 g |
Ask for price |
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Gel Tray |
2394250 |
Atto |
4unit |
EUR 324 |
Description: I ncl udi ng pl at es |
Gel Tray |
2398194 |
Atto |
2unit |
EUR 424.8 |
TC Gel |
CP048-005 |
GenDepot |
500 g |
EUR 278.4 |
TC Gel |
CP048-010 |
GenDepot |
1 Kg |
EUR 416.4 |
Gel Cutter |
KS071012-11 |
Biochain |
12 |
EUR 106.8 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Gel Filter |
KS071012-13 |
Biochain |
12 |
EUR 138 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Rabbit Anti-E. Coli Proteins IgG-Agarose affinity gel for removing E. coli proteins |
EC12-AS |
Alpha Diagnostics |
1 ml |
EUR 634.8 |
Precast Agarose Gel TAE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box |
TAE12-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TAE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box |
TAE17-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TAE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box |
TAE48-2PER-SF |
Bio Basic |
1BOX, 10UNIT |
EUR 440.02 |
|
Precast Agarose Gel TBE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box |
TBE12-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TBE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box |
TBE17-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TBE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box |
TBE48-2PER-SF |
Bio Basic |
1BOX, 10UNIT |
EUR 440.02 |
|
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
electrophoresis ps 400v, 500ma, 50w |
EV1450 |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
EV1450-UK |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
EV1450-US |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230 |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230-UK |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230-US |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 300v, 1000ma, 150w |
EV2310 |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
EV2310-UK |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
EV2310-US |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 3000v, 150ma, 150w |
EV2320 |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
EV2320-UK |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
EV2320-US |
Consort |
ea |
EUR 898.8 |
electrophoresis power supply uk plug |
EV245-UK |
Consort |
ea |
EUR 574.8 |
electrophoresis power supply 120 vac |
EV245-US |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 600v, 500ma, 150w |
EV2650 |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
EV2650-UK |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
EV2650-US |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 300v, 2000ma, 300w |
EV3020 |
Consort |
ea |
EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
EV3020-UK |
Consort |
ea |
EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
EV3020-US |
Consort |
ea |
EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150 |
Consort |
ea |
EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150-UK |
Consort |
ea |
EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150-US |
Consort |
ea |
EUR 960 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330 |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330-UK |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330-US |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610 |
Consort |
ea |
EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610-UK |
Consort |
ea |
EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610-US |
Consort |
ea |
EUR 960 |
electrophoresis ps 6000v, 150ma, 300w |
EV3620 |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
EV3620-UK |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
EV3620-US |
Consort |
ea |
EUR 1456.8 |
PCR Mycoplasma Detection Kit |
M034-Kit |
TOKU-E |
Kit |
EUR 319.2 |
AE-6111 Agarose EP Kit |
2322178 |
Atto |
1unit |
EUR 1035.6 |
Description: 1 el ect r ophor esi s chamber i ncl udi ng. safet y l i dwi t h power l eads, a 12 x 16 cm gel t r ay wi t h 2 dams, 1 comb hol der and 1 comb for 1 mm, 26 & |
Immunoprecipitation Kit: Protein A-Agarose |
BS688 |
Bio Basic |
20Rxn, 20prep |
EUR 242.7 |
|
SDS-PAGE Gel Preparation Kit |
abx090646-1Kit |
Abbexa |
1 Kit |
EUR 243.6 |
|
Gel Extraction Kit (100 preps) |
9K-006-0001 |
Bio Basic |
100preps |
EUR 283.42 |
|
Gel Extraction Kit (200 preps) |
9K-006-0002 |
Bio Basic |
200preps, 200prep |
EUR 418.09 |
|
ISOLATE II PCR & Gel Kit |
BIO-52058 |
Bioline |
10 preps |
Ask for price |
ISOLATE II PCR & Gel Kit |
BIO-52059 |
Bioline |
50 preps |
Ask for price |
ISOLATE II PCR & Gel Kit |
BIO-52060 |
Bioline |
250 preps |
Ask for price |
SDS-PAGE Gel Preparation Kit |
AR0138 |
BosterBio |
1kit (Enough for 30-50 pieces of gel.) |
EUR 115.2 |
GEL Purification Mini Kit (50prep) |
FAGPK-001 |
Favorgen |
50 preps |
EUR 144 |
GEL Purification Mini Kit (200prep) |
FAGPK-001-1 |
Favorgen |
200 preps |
EUR 202.8 |
GEL Purification Mini Kit (300prep) |
FAGPK-001-2 |
Favorgen |
300 preps |
EUR 226.8 |
MicroElute GEL Purification Kit (50prep) |
FAMGK-001 |
Favorgen |
50 preps |
EUR 151.2 |
MicroElute GEL Purification Kit (200prep) |
FAMGK-001-1 |
Favorgen |
200 preps |
EUR 224.4 |
Zenoquick Gel DNA Recovery Kit |
Z3001-050 |
GenDepot |
50 preps |
EUR 238.8 |
Zenoquick Gel DNA Recovery Kit |
Z3001-200 |
GenDepot |
4x50 preps |
EUR 757.2 |
FitAmp Gel DNA Isolation Kit |
P-1007 |
EpiGentek |
100 Samples |
EUR 324.48 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
4250-050-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
4250-050-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
4250-050-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs |
ELE2300 |
Scientific Laboratory Supplies |
EACH |
EUR 1448.94 |
The results showed that there was a close relationship between environmental and clinical isolates A. Baumannii. Cross contamination is also very important that occurs through daily clinical activities between environmental and clinical isolates. Therefore, to reduce Klonal Contamination MDR A. Baumannii environment and clinical isolates, it is necessary to use strict infection control strategies.