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A method combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem-mass spectrometry to detect circulating immune complexes between therapeutic monoclonal antibodies and anti-drug antibodies in animals.

A method combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem-mass spectrometry to detect circulating immune complexes between therapeutic monoclonal antibodies and anti-drug antibodies in animals.
Posted by Troy

Therapeutic monoclonal antibodies have the potential to cause an unwanted immune response, resulting in anti-drug antibody production (fennel). Fennel binding to drugs and subsequent formation of the immune complex (ICS) can trigger various responses, depending on the size, concentration, and subclass of Agas. To better understand the impact of Agas on pharmacokinetics, pharmacodynamics, and toxicological profiles, the bioanalysis method was developed to detect ICS between the Immunoglobulin G (IGG) of humans and Agas in biological samples.

Regarding experimental procedures, briefly, the specific ICS of human antibodies and human antibodies that are not bound in biological samples are separated through electrophoresis of original blue polyacrylamide gel (BN-PA-Page). The target fraction is then cut from the gel, followed by the digestion of Trypsin in the gel and liquid chromatography then the spectrometry of the spectrometry (LC-MS / MS) to monitor human specific peptides. This method is able to detect various types of human antibodies with lower detection limits than 10 μg / ml in serum monkeys. The performance of testing for ICS detection is shown using barbed samples, and pre-incubation ICs in the monkey serum are clearly detected.

Taken together, these findings indicate that our method allows semi quantitative analysis to estimate the ratio of human antibodies including IC compared to total antibodies. This method was successfully applied to the study in Vivo using mice, and data helped explain unexpectedly cleaning antibodies due to large IC formation. The combination of ICS separation by BN-PAGE and Human IgG specific peptide detection by LC-MS / MS is a common bioanalytical approach that is useful for Detection of ICS in animals.

The characterization of the eubacterial community by denying the gradient gel electrophoresis (DGGE) and the next generation sequencing (NGS) in the biotrickling deculfurization filter using progressive changes in nitrate and nitrite as the final electron acceptor.

Anoxic biotrickling filters (BTFS) represent the technology with high H2S elimination capacity and removal efficiency which is widely studied for biogas desulfurization. Three changes in the final electron acceptor are made using nitrate and nitrite during the operating period of 520 days. BTF’s anoxic stability and performance is maintained when a significant disruption is applied to a system involving progressive changes in nitrate to nitrite and vice versa.

Here the impact of changes in electron acceptors in the microbial community are characterized by denaturation of electrophoresis (DGGE) gradient gel and the next generation (NGS) sequencing. Both platforms revealed that the community underwent changes during perturbations but was tough because the transfer capacity did not significantly change. Proteobacteria and bacteroides are the main phylum and sulfurimonas and Tiobacillus which are the oxidizing genera-oxidizing-oxidizing (NR-SOB) involved in the biodesulfurization process

Monitoring the plasmid vaccine topology with capillary gel electrophoresis.

Plasmid DNA has been widely used in vaccination as well as in cells and gen therapy. It exists in some isoforms including supercoiled, scelled or open circular and linear forms. Regulatory institutions recommend having more than 80% of supercoiled isoforms for mass release plasmid products; Thus must be analyzed accordingly. The traditional analysis method for Plasmid DNA is Gel Gel Electrophoresis. However, because manual sample loading, visualization, and analysis of manual data, it has limitations in obtaining consistent quantitative results.

A method combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem-mass spectrometry to detect circulating immune complexes between therapeutic monoclonal antibodies and anti-drug antibodies in animals.

In this brief communication, we introduce a fast, sensitive, and powerful plasmid analysis method using capillary electrophoresis with fluorescence detection of laserinduced (CE-LIF). CE-LIF analysis of the isoform Supercoiled and open circle partners are finished in 20 minutes with excellent sensitivity by using the dye binding the same neon flow. The advantage of this method is indicated by the purity analysis of two large plasmids (7 KB and 10 KB). Sample loading, separation, and completely automatic data analysis display repetition of improved tests and ease of quantization of gel electrophoresis gel agarose degradation.

Molecular typing acinetobacter baumannii which resistant drugs from clinical and environmental specimens in three Iranian hospitals with pulsed field gel electrophoresis.

Multi-Medicine Resistant (MDR) Acinetobacter Baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and clinical relations of clinical and environmental isolates A. Baumannii with the electrophoresis method of field gel pulsed. Forty-three clinical and 26 environmental isolates from MDR A. Baumannii was collected and recognized through 20ne fire. Antibiotic resistance of isolates is assessed by disk diffusion method, and biofilm formation tests are carried out by the microtiter plate method. Field gel electrophoresis (PFGE) is used to assess the genome features of bacterial isolates.

The resistance level of clinical and environmental isolates to antibiotics comes from 95 to 100%. The difference between antibiotic resistance between clinical and environmental isolates is not statistically significant (p> 0.05). The ability of biofilm production reveals that 31 (44.9%), and 30 (43.5%) isolates have strong and moderate biofilm producers activities. Typing PFGGes shows eight different clusters (A, B, C, D, E, F, G, and H) with two significant clusters including A and G with 21 (30.4%) and 16 (23.2%) each -Masing, which consists of 53.6% of all isolates. There is no relationship between biofilm formation and antibiotic resistance patterns with pulsotypes of PFGE.

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
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Agarose gel DNA extraction and purification kit

K1441-100
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Agarose Gel DNA Extraction and Purification

EP10010 100 Tests
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ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
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ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
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Cys-link agarose gel coupling buffer kit (5-10 peptides)

110400-CB 1 kit
EUR 225

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
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Gel-FAST? Gel Staining/Destaining Kit

K901-40
EUR 175

electrophoresis power supply

EV245 ea
EUR 479

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 2950.8

Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins)

110100-CG 5 ml
EUR 286

Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins)

110200-NG 5 ml
EUR 286

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies

EC11-G 1 ml
EUR 286

Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies

KLH11-G 1 ml
EUR 225

Agarose

20-abx186369
  • EUR 509.00
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  • 100 g
  • 25 g

Agarose

20-abx098147
  • EUR 217.00
  • EUR 105.00
  • 100 g
  • 1 g

Agarose

abx082006-100g 100 g
EUR 244

Agarose

abx082007-100g 100 g
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Agarose

abx082008-100g 100 g
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Agarose

G060-1 100 g
EUR 176

Agarose

G060-2 500 g
EUR 369

Agarose

Z5050007 500 g
EUR 615
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Agarose

Z5050013 100 g
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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 185

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1587

Gel-Bright LED Gel Illuminator

E90003 1EA
EUR 773
Description: Minimum order quantity: 1 unit of 1EA

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
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Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
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Starch (Smithies), for electrophoresis

GE6857-500G 500 g
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Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
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ES Horizontal Electrophoresis Set

LE1011 Set
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myGel Mini Electrophoresis System

BM0286 System
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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

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Precast Agarose Gel TAE, 2%, 12 Well, EtBr Stained, 20 gels per Box

TAE12-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TAE, 2%, 17 Well, EtBr Stained, 20 gels per Box

TAE17-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TAE, 2%, 48 Well, EtBr Stained, 10 gels per Box

TAE48-2PER-EB 1BOX, 10UNIT
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Precast Agarose Gel TBE, 2%, 12 Well, EtBr Stained, 20 gels per Box

TBE12-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 17 Well, EtBr Stained, 20 gels per Box

TBE17-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 48 Well, EtBr Stained, 10 gels per Box

TBE48-2PER-EB 1BOX, 10UNIT
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Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies

KLH11-G-5 5 ml
EUR 529

Gel DNA Recovery Kit

GP05 5 preps Ask for price

Gel DNA Recovery Kit

GP100 100 preps
EUR 130

Gel DNA Recovery Kit

GP200 200 preps
EUR 189

Gel DNA Recovery Kit

GP50 50 preps
EUR 94

AmbiClean Kit (PCR & Gel)

GV05 5 preps Ask for price

AmbiClean Kit (PCR & Gel)

GV100 100 preps
EUR 128

AmbiClean Kit (PCR & Gel)

GV200 200 preps
EUR 189

AmbiClean Kit (PCR & Gel)

GV50 50 preps
EUR 91

Quick Gel Extraction Kit

20-abx098085
  • EUR 286.00
  • EUR 203.00
  • 200 rxns
  • 50 rxns

TC Gel

CP048-005 500 g
EUR 232

TC Gel

CP048-010 1 Kg
EUR 347

Gel Tray

2394250 4unit
EUR 270
Description: I ncl udi ng pl at es

Gel Tray

2398194 2unit
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Gel Cutter

KS071012-11 12
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Gel Filter

KS071012-13 12
EUR 115
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

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D0012 50g
EUR 80.45

Agarose StandardRoomTemperature

FYA002-100G 100 g Ask for price

Agarose StandardRoomTemperature

FYA002-500G 500 g Ask for price

Agarose, Sepro

A0224-010 100g
EUR 219

Agarose, Sepro

A0224-020 200g
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Agarose, Sepro

A0224-050 500g
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Agarose Tablets

BA50201 100pcs
EUR 109
Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose M

AB0013 50g
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Agarose L.M.P

20-abx082386
  • EUR 272.00
  • EUR 217.00
  • 10 g
  • 5 g

Agarose LE

A-1270-100 100 g
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Agarose LE

A-1270-500 500 g
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Agarose HR

A-1280-100 100 g
EUR 183

Agarose HR

A-1280-500 500 g
EUR 685

Agarose GA

A-1290-100 100 g
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Agarose GA

A-1290-500 500 g
EUR 255

Agarose LM

A-1300-100 100 g
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Agarose LM

A-1300-25 25 g
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Precast Agarose Gel TAE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box

TAE12-2PER-SF 1BOX, 20UNIT
EUR 286.2

Precast Agarose Gel TAE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box

TAE17-2PER-SF 1BOX, 20UNIT
EUR 286.2

Precast Agarose Gel TAE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

TAE48-2PER-SF 1BOX, 10UNIT
EUR 366.68

Precast Agarose Gel TBE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box

TBE12-2PER-SF 1BOX, 20UNIT
EUR 286.2

Precast Agarose Gel TBE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box

TBE17-2PER-SF 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

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Rabbit Anti-E. Coli Proteins IgG-Agarose affinity gel for removing E. coli proteins

EC12-AS 1 ml
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electrophoresis ps 400v, 500ma, 50w

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electrophoresis ps 400v, 500ma, 50w

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electrophoresis ps 400v, 500ma, 50w

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electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
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electrophoresis ps 1500v, 300ma, 150w

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electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
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electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
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electrophoresis ps 300v, 1000ma, 150w

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electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
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electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
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electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
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electrophoresis power supply uk plug

EV245-UK ea
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electrophoresis power supply 120 vac

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electrophoresis ps 600v, 500ma, 150w

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electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
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electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
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electrophoresis ps 300v, 2000ma, 300w

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electrophoresis ps 300v, 2000ma, 300w

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electrophoresis ps 300v, 2000ma, 300w

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electrophoresis ps 1200v, 500ma, 300w

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electrophoresis ps 1200v, 500ma, 300w

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electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
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electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
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electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
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electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
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electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
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electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
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electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
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5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

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P-1007 100 Samples
EUR 270.4
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FAGPK-001 50 preps
EUR 120

GEL Purification Mini Kit (200prep)

FAGPK-001-1 200 preps
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FAGPK-001-2 300 preps
EUR 189

MicroElute GEL Purification Kit (50prep)

FAMGK-001 50 preps
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FAMGK-001-1 200 preps
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ISOLATE II PCR & Gel Kit

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9K-006-0001 100preps
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9K-006-0002 200preps, 200prep
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SDS-PAGE Gel Preparation Kit

AR0138 1kit (Enough for 30-50 pieces of gel.)
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SDS-PAGE Gel Preparation Kit

abx090646-1Kit 1 Kit
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Zenoquick Gel DNA Recovery Kit

Z3001-050 50 preps
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Zenoquick Gel DNA Recovery Kit

Z3001-200 4x50 preps
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Immunoprecipitation Kit: Protein A-Agarose

BS688 20Rxn, 20prep
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AE-6111 Agarose EP Kit

2322178 1unit
EUR 863
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PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

ViSafe RED Gel

S430 500 µl
EUR 146

ViSafe RED Gel

S430L 5x500 µl
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S435 500 µl
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ViSafe Green Gel

S435L 5x500 µl
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Ketanserin (Vulketan Gel)

E1KS2232 50mg
EUR 434

Aluminium Phosphate Gel

VAdv-Ly0001 250 mL
EUR 1386
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Aluminium Hydroxide Gel

VAdv-Ly0003 250 mL
EUR 1386
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Gel tray L

2322112 5unit
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Gel tray S

2322117 5unit
EUR 257
Description: 2pcs/1set

The results showed that there was a close relationship between environmental and clinical isolates A. Baumannii. Cross contamination is also very important that occurs through daily clinical activities between environmental and clinical isolates. Therefore, to reduce Klonal Contamination MDR A. Baumannii environment and clinical isolates, it is necessary to use strict infection control strategies.

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