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A method combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem-mass spectrometry to detect circulating immune complexes between therapeutic monoclonal antibodies and anti-drug antibodies in animals.

A method combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem-mass spectrometry to detect circulating immune complexes between therapeutic monoclonal antibodies and anti-drug antibodies in animals.
Posted by Troy

Therapeutic monoclonal antibodies have the potential to cause an unwanted immune response, resulting in anti-drug antibody production (fennel). Fennel binding to drugs and subsequent formation of the immune complex (ICS) can trigger various responses, depending on the size, concentration, and subclass of Agas. To better understand the impact of Agas on pharmacokinetics, pharmacodynamics, and toxicological profiles, the bioanalysis method was developed to detect ICS between the Immunoglobulin G (IGG) of humans and Agas in biological samples.

Regarding experimental procedures, briefly, the specific ICS of human antibodies and human antibodies that are not bound in biological samples are separated through electrophoresis of original blue polyacrylamide gel (BN-PA-Page). The target fraction is then cut from the gel, followed by the digestion of Trypsin in the gel and liquid chromatography then the spectrometry of the spectrometry (LC-MS / MS) to monitor human specific peptides. This method is able to detect various types of human antibodies with lower detection limits than 10 μg / ml in serum monkeys. The performance of testing for ICS detection is shown using barbed samples, and pre-incubation ICs in the monkey serum are clearly detected.

Taken together, these findings indicate that our method allows semi quantitative analysis to estimate the ratio of human antibodies including IC compared to total antibodies. This method was successfully applied to the study in Vivo using mice, and data helped explain unexpectedly cleaning antibodies due to large IC formation. The combination of ICS separation by BN-PAGE and Human IgG specific peptide detection by LC-MS / MS is a common bioanalytical approach that is useful for Detection of ICS in animals.

The characterization of the eubacterial community by denying the gradient gel electrophoresis (DGGE) and the next generation sequencing (NGS) in the biotrickling deculfurization filter using progressive changes in nitrate and nitrite as the final electron acceptor.

Anoxic biotrickling filters (BTFS) represent the technology with high H2S elimination capacity and removal efficiency which is widely studied for biogas desulfurization. Three changes in the final electron acceptor are made using nitrate and nitrite during the operating period of 520 days. BTF’s anoxic stability and performance is maintained when a significant disruption is applied to a system involving progressive changes in nitrate to nitrite and vice versa.

Here the impact of changes in electron acceptors in the microbial community are characterized by denaturation of electrophoresis (DGGE) gradient gel and the next generation (NGS) sequencing. Both platforms revealed that the community underwent changes during perturbations but was tough because the transfer capacity did not significantly change. Proteobacteria and bacteroides are the main phylum and sulfurimonas and Tiobacillus which are the oxidizing genera-oxidizing-oxidizing (NR-SOB) involved in the biodesulfurization process

Monitoring the plasmid vaccine topology with capillary gel electrophoresis.

Plasmid DNA has been widely used in vaccination as well as in cells and gen therapy. It exists in some isoforms including supercoiled, scelled or open circular and linear forms. Regulatory institutions recommend having more than 80% of supercoiled isoforms for mass release plasmid products; Thus must be analyzed accordingly. The traditional analysis method for Plasmid DNA is Gel Gel Electrophoresis. However, because manual sample loading, visualization, and analysis of manual data, it has limitations in obtaining consistent quantitative results.

A method combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem-mass spectrometry to detect circulating immune complexes between therapeutic monoclonal antibodies and anti-drug antibodies in animals.

In this brief communication, we introduce a fast, sensitive, and powerful plasmid analysis method using capillary electrophoresis with fluorescence detection of laserinduced (CE-LIF). CE-LIF analysis of the isoform Supercoiled and open circle partners are finished in 20 minutes with excellent sensitivity by using the dye binding the same neon flow. The advantage of this method is indicated by the purity analysis of two large plasmids (7 KB and 10 KB). Sample loading, separation, and completely automatic data analysis display repetition of improved tests and ease of quantization of gel electrophoresis gel agarose degradation.

Molecular typing acinetobacter baumannii which resistant drugs from clinical and environmental specimens in three Iranian hospitals with pulsed field gel electrophoresis.

Multi-Medicine Resistant (MDR) Acinetobacter Baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and clinical relations of clinical and environmental isolates A. Baumannii with the electrophoresis method of field gel pulsed. Forty-three clinical and 26 environmental isolates from MDR A. Baumannii was collected and recognized through 20ne fire. Antibiotic resistance of isolates is assessed by disk diffusion method, and biofilm formation tests are carried out by the microtiter plate method. Field gel electrophoresis (PFGE) is used to assess the genome features of bacterial isolates.

The resistance level of clinical and environmental isolates to antibiotics comes from 95 to 100%. The difference between antibiotic resistance between clinical and environmental isolates is not statistically significant (p> 0.05). The ability of biofilm production reveals that 31 (44.9%), and 30 (43.5%) isolates have strong and moderate biofilm producers activities. Typing PFGGes shows eight different clusters (A, B, C, D, E, F, G, and H) with two significant clusters including A and G with 21 (30.4%) and 16 (23.2%) each -Masing, which consists of 53.6% of all isolates. There is no relationship between biofilm formation and antibiotic resistance patterns with pulsotypes of PFGE.

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
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Agarose gel DNA extraction and purification kit

K1441-100 each
EUR 510

Agarose Gel DNA Extraction and Purification

EP10010 100 Tests
EUR 356.4

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

Cys-link agarose gel coupling buffer kit (5-10 peptides)

110400-CB 1 kit
EUR 270

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

Gel-FAST? Gel Staining/Destaining Kit

K901-40 each
EUR 210

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

electrophoresis power supply

EV245 ea
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miniONE Electrophoresis System

ELE3400 EACH
EUR 624.72

Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins)

110100-CG 5 ml
EUR 343.2

Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins)

110200-NG 5 ml
EUR 343.2

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies

EC11-G 1 ml
EUR 343.2

Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies

KLH11-G 1 ml
EUR 270

Agarose

abx082006-100g 100 g
EUR 292.8

Agarose

abx082007-100g 100 g
EUR 309.6

Agarose

abx082008-100g 100 g
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Agarose

20-abx098147
  • EUR 260.40
  • EUR 126.00
  • 100 g
  • 1 g

Agarose

20-abx186369
  • EUR 610.80
  • EUR 360.00
  • 100 g
  • 25 g

Agarose

G060-1 100 g
EUR 211.2

Agarose

G060-2 500 g
EUR 442.8

Agarose

Z5050007 500 g
EUR 738
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Agarose

Z5050013 100 g
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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 222

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1904.4

Gel-Bright LED Gel Illuminator

E90003 1EA
EUR 927.6
Description: Minimum order quantity: 1 unit of 1EA

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

ES Horizontal Electrophoresis Set

LE1011 Set
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Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
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Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
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Starch (Smithies), for electrophoresis

GE6857-500G 500 g
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Chiller for Electrophoresis systems

CSLCHILLER EACH
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myGel Mini Electrophoresis System

E1101 1 each
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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies

KLH11-G-5 5 ml
EUR 634.8

Precast Agarose Gel TAE, 2%, 12 Well, EtBr Stained, 20 gels per Box

TAE12-2PER-EB 1BOX, 20UNIT
EUR 280.8

Precast Agarose Gel TAE, 2%, 17 Well, EtBr Stained, 20 gels per Box

TAE17-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TAE, 2%, 48 Well, EtBr Stained, 10 gels per Box

TAE48-2PER-EB 1BOX, 10UNIT
EUR 331.44

Precast Agarose Gel TBE, 2%, 12 Well, EtBr Stained, 20 gels per Box

TBE12-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 17 Well, EtBr Stained, 20 gels per Box

TBE17-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 48 Well, EtBr Stained, 10 gels per Box

TBE48-2PER-EB 1BOX, 10UNIT
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Quick Gel Extraction Kit

20-abx098085
  • EUR 343.20
  • EUR 243.60
  • 200 rxns
  • 50 rxns

Gel DNA Recovery Kit

GP05 5 preps Ask for price

Gel DNA Recovery Kit

GP100 100 preps
EUR 156

Gel DNA Recovery Kit

GP200 200 preps
EUR 226.8

Gel DNA Recovery Kit

GP50 50 preps
EUR 112.8

AmbiClean Kit (PCR & Gel)

GV05 5 preps Ask for price

AmbiClean Kit (PCR & Gel)

GV100 100 preps
EUR 153.6

AmbiClean Kit (PCR & Gel)

GV200 200 preps
EUR 226.8

AmbiClean Kit (PCR & Gel)

GV50 50 preps
EUR 109.2

Agarose, Sepro

A0224-010 100g
EUR 262.8

Agarose, Sepro

A0224-020 200g
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Agarose, Sepro

A0224-050 500g
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Agarose L.M.P

20-abx082386
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  • 10 g
  • 5 g

Agarose M

AB0013 50g
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Agarose LE

A-1270-100 100 g
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Agarose LE

A-1270-500 500 g
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A-1280-100 100 g
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Agarose GA

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Agarose GA

A-1290-500 500 g
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Agarose LM

A-1300-25 25 g
EUR 123.6

Agarose Tablets

BA50201 100pcs
EUR 130.8
Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose A

D0012 50g
EUR 96.54

Agarose StandardRoomTemperature

FYA002-100G 100 g Ask for price

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Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Gel Tray

2394250 4unit
EUR 324
Description: I ncl udi ng pl at es

Gel Tray

2398194 2unit
EUR 424.8

TC Gel

CP048-005 500 g
EUR 278.4

TC Gel

CP048-010 1 Kg
EUR 416.4

Gel Cutter

KS071012-11 12
EUR 106.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Gel Filter

KS071012-13 12
EUR 138
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Rabbit Anti-E. Coli Proteins IgG-Agarose affinity gel for removing E. coli proteins

EC12-AS 1 ml
EUR 634.8

Precast Agarose Gel TAE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box

TAE12-2PER-SF 1BOX, 20UNIT
EUR 343.44

Precast Agarose Gel TAE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box

TAE17-2PER-SF 1BOX, 20UNIT
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Precast Agarose Gel TAE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

TAE48-2PER-SF 1BOX, 10UNIT
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Precast Agarose Gel TBE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box

TBE12-2PER-SF 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box

TBE17-2PER-SF 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

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Column Packing Kit

PACK-KIT 1pack
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
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electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
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electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
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electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
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electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
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electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

electrophoresis power supply 120 vac

EV245-US ea
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electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
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electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
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electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
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electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
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electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
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electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
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electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
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EV3620 ea
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electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
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electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
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5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 128.4

PCR Mycoplasma Detection Kit

M034-Kit Kit
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AE-6111 Agarose EP Kit

2322178 1unit
EUR 1035.6
Description: 1 el ect r ophor esi s chamber i ncl udi ng. safet y l i dwi t h power l eads, a 12 x 16 cm gel t r ay wi t h 2 dams, 1 comb hol der and 1 comb for 1 mm, 26 &

Immunoprecipitation Kit: Protein A-Agarose

BS688 20Rxn, 20prep
EUR 242.7

SDS-PAGE Gel Preparation Kit

abx090646-1Kit 1 Kit
EUR 243.6

Gel Extraction Kit (100 preps)

9K-006-0001 100preps
EUR 283.42

Gel Extraction Kit (200 preps)

9K-006-0002 200preps, 200prep
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ISOLATE II PCR & Gel Kit

BIO-52058 10 preps Ask for price

ISOLATE II PCR & Gel Kit

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ISOLATE II PCR & Gel Kit

BIO-52060 250 preps Ask for price

SDS-PAGE Gel Preparation Kit

AR0138 1kit (Enough for 30-50 pieces of gel.)
EUR 115.2

GEL Purification Mini Kit (50prep)

FAGPK-001 50 preps
EUR 144

GEL Purification Mini Kit (200prep)

FAGPK-001-1 200 preps
EUR 202.8

GEL Purification Mini Kit (300prep)

FAGPK-001-2 300 preps
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MicroElute GEL Purification Kit (50prep)

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MicroElute GEL Purification Kit (200prep)

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Zenoquick Gel DNA Recovery Kit

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Zenoquick Gel DNA Recovery Kit

Z3001-200 4x50 preps
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Gel Filtration LMW Calibration Kit

28403841 EACH
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Gel Filtration HMW Calibration Kit

28403842 EACH
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FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1448.94

The results showed that there was a close relationship between environmental and clinical isolates A. Baumannii. Cross contamination is also very important that occurs through daily clinical activities between environmental and clinical isolates. Therefore, to reduce Klonal Contamination MDR A. Baumannii environment and clinical isolates, it is necessary to use strict infection control strategies.

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