A recent study found that the method of gel electrophoresis (age) produces two different big ribbons that match the isoenzyme hepatic and bone Alp (Alp2 and Alp3, Respec-tive) in the serum of cattle treated with protease and neuraminidase (PN- Treatment), although there are concerns that Alp Isoenzyme intestine (Alp5) often overlaps with Alp3 on serum humans treated with neuraminidase. Because the Alp5 is separated from Alp3 in the serum of cattle treated with Protease only (P-Treatment), we use modified methods using Sera Bovine treated with PN and PN to measure the activity of three ISOenzyme Alp in 53 cattle Lacta-Ting Holstein: 24 primipara and 29 multipara.
After electrophoresis, 51 of 53 samples (96.2%) experienced P treatment that produced different fractions that fit alp5, as did serum control. All Sera treated by PN produces a sure Alp2 faction. The Alp3 faction is calculated as the rest after excluding Alp2 and Alp5. The total activities of ALP (T-ALP) and Alp3 in primipar beef are higher than those in the Multipara cow (P ⟨0,001) at the beginning to the top [10-110 days in milk (dim)] and mid (111-220) dim) lactation. In multi-parous cattle, alp3 activity in late lactation (221-477 dim) is significantly higher than it is on initial lactation to the top. Thus, the modified age method described here is capable of distinguishing three fractions of ISOEENZIM ALP in serum cattle lacting. The age pattern of ISOENZIM ALP Circu-Lating will contribute to understanding the status of physiological bone metabolism in breastfeeding cattle.
Increased quantification of aggregate covalent protein therapy with non-reducer capillary gel electrophoresis using sodium hexadecyl sulfate (CE-SHS)
Capillary gel electrophoresis (CGE) uses sodium dodecyl sulfate (CGE-SDS or CE-SDS) is generally used in the biotechnology industry to assess complex therapy purity during optimization of the manufacturing process and also for testing commercial releases and stability. However, for MAB-1 and MAB-2 therapy proteins, non-reduction (NR) produce higher than the expected% aggregate which significantly reduces its purity relative to the purity reported by other complementary methods, such as the size chromatography (seconds).
Furthermore, the dependence of strong protein burden on aggregate levels is observed that prevents reasonable assessment of true purity values. The solution is to complete the SDS with a relatively hydrophobic sodium hexadecyl sulfate detergent (SHS) in a sifting gel buffer matrix that almost eliminates the dependence of the protein load and reduces the% aggregate value to the expected level when compared to the SEC. Analytical Ultracentrugation (AUC) is used to help confirm the accuracy of the SEC results. This work underlines how to use detergents in addition to SDS in the CGE application can be valuable in the commercial biology room and provide an example of how the SEC can be used to confirm the accuracy of CGE data.
Low-Abundance Lisozyme Purification in Egg White Through Free Flow Electrophoresis with Gel Filtration Chromatography
As an effective separation tool, free flow electrophoresis has not been used for abundant low protein purification in a complex sample matrix. Here, the lysozyme in the complex egg white matrix is chosen as a protein model to demonstrate low content peptide purification through the FFE coupled with gel (GFC) chromatography. Raw lisozyme in eggs when first separated through free flow zone electrophoresis (FFZE). After that, the fraction with lysozyme activity is condensed through lyophilization. After that, the condensed fraction is more sanctified through the Sephadex G50 GFC. In all experiments, special poly (acid acramide-co-acrylic) (P (AM-CO-AA)) electrophoresis and mass spectrometry are used for identification of lysozymes.

FFZE conditions are optimized as follows: 130 μL / min the sample flow rate, 4.9 ml / min background buffer 20 mm pH 5.5 tris-acetate acid, 350 V, and 14 ° C and 2 ml / ml protein content. rough sample. It was found that pure lysozyme had 80% purity and high activity compared to the rough sample only with 1.4% content and undetectable activities. Recovery in the first and second separative steps is 65% and 82%, and the total recovery of around 53.3%. Low recovery reasons can be induced by Lisozyme diffusion out of gel (AM-CO-AA) gel and eliminate ovalbumin abundant eggs. All of these results indicated by FFE can be used as an alternative tool for purification of the target of solute with low abundance.
The n-glycan structure of the target biomarker cancer is marked with two-dimensional gel electrophoresis and mass spectrometry
Glycoprotein is an important biomarker for cancer, while most of the glycoprotomic biomarkers suffer from low sensitivity and specificity because of the structure of glycan that is not neglected. AZGP1 is a potential biomarker for the diagnosis of lung cancer salivary, which is used as a glycoprotein model in this study for the development of the method. We initially analyzed Saliva n-glycoprote using literary affinity chromatography and more than 300 n-glycoproteins were identified, including AZGP1. 7 AZGP1 gel spots are completed with two-dimensional gel electrophoresis gel and subsequently confirmed by two-dimensional Western blots and mass spectrometry.
The glycan isomer structure of AZGP1 at these points systematically characterizes both the composition level and at the level of structure. Our results revealed 10 glycan compositions for AZGP1 saliva, including the glycans fucosylated core on ASN128 and Glyylated Glycans at ASN109 and ASN112. We then compared the glycan structure of AZGP1 saliva from the lung cancer group and the control group.
electrophoresis ps 1500v, 300ma, 150w |
EV2230 |
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ea |
EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230-UK |
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EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
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EUR 898.8 |
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EUR 898.8 |
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EUR 898.8 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150 |
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EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150-UK |
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EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
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EUR 960 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330 |
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EUR 1456.8 |
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EUR 1456.8 |
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EUR 1456.8 |
electrophoresis ps 400v, 500ma, 50w |
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EUR 574.8 |
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EUR 574.8 |
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electrophoresis ps 300v, 1000ma, 150w |
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EUR 686.4 |
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EUR 686.4 |
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EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EUR 686.4 |
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EUR 686.4 |
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EUR 686.4 |
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EUR 960 |
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EUR 960 |
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EUR 960 |
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EV3610 |
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EUR 960 |
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EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610-US |
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ea |
EUR 960 |
electrophoresis power supply |
EV245 |
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ea |
EUR 574.8 |
CometAssay Electrophoresis System |
4250-050-ES |
Biotechne |
1 Kit |
EUR 3540.96 |
myGel Mini Electrophoresis System |
BM0286 |
GenDepot |
System |
EUR 741.6 |
ES Horizontal Electrophoresis Set |
LE1011 |
GenDepot |
Set |
EUR 578.4 |
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380 |
ACT-CBMN-7 |
ACTGene |
each |
EUR 892.8 |
6X6 well Electrophoresis Apparatus |
EDK626 |
Bio Basic |
1 UNIT |
EUR 750.6 |
|
electrophoresis power supply 120 vac |
EV245-US |
Consort |
ea |
EUR 574.8 |
electrophoresis power supply uk plug |
EV245-UK |
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ea |
EUR 574.8 |
ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels |
LE1001 |
GenDepot |
Set |
EUR 651.6 |
CometAssay Electrophoresis Starter Kit |
4250-050-ESK |
Biotechne |
1 Kit |
EUR 3749.28 |
ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels |
LE1002 |
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Set |
EUR 699.6 |
ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels |
LE1003 |
GenDepot |
Set |
EUR 586.8 |
ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels |
LE1004 |
GenDepot |
Set |
EUR 886.8 |
ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels |
LE1005 |
GenDepot |
Set |
EUR 1081.2 |
ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels |
LE1006 |
GenDepot |
Set |
EUR 1170 |
ES Electrophoresis Casting Tray, 7cm X 7cm |
LE1011-A2 |
GenDepot |
Ea |
EUR 181.2 |
ES Electrophoresis Casting Tray, 7cm X 14cm |
LE1011-A1 |
GenDepot |
Ea |
EUR 198 |
Electrophoresis Apparatus - 7x10cm (6-10-well) |
EDK611 |
Bio Basic |
1 UNIT |
EUR 383.11 |
|
Electrophoresis Apparatus - 7x14cm (6-10-well) |
EDK613 |
Bio Basic |
1 UNIT |
EUR 487.51 |
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Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well) |
EDK614 |
Bio Basic |
1 UNIT |
EUR 520.92 |
|
10-well protein Vertical Electrophoresis Apparatus |
EDK692 |
Bio Basic |
1 UNIT |
EUR 487.51 |
|
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs |
ELE2300 |
Scientific Laboratory Supplies |
EACH |
EUR 1602 |
myGel Mini Electrophoresis System 230V (includes transformer) |
BM0291 |
GenDepot |
System |
EUR 789.6 |
myGel Mini Electrophoresis System 230V (includes transformer) |
E1101-E |
Benchmark Scientific |
1 PC |
EUR 652.5 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
4250-050-ESK-PS2 |
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1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
4250-050-ESK-PS3 |
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1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
4250-050-ESK-PS4 |
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1 Kit |
EUR 4597.68 |
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs |
ELE2302 |
Scientific Laboratory Supplies |
EACH |
EUR 1681.2 |
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator |
E1201 |
Benchmark Scientific |
1 each |
EUR 1001.7 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100) |
E1101-SK |
Benchmark Scientific |
1 each |
EUR 712.8 |
OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules |
VS10WCES |
Scientific Laboratory Supplies |
EACH |
EUR 1434.85 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100) |
E1101-SK-E |
Benchmark Scientific |
1 PC |
EUR 742.5 |
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input |
E1201-E |
Benchmark Scientific |
1 each |
EUR 1058.9 |
Agarose, Low melting gel |
AB0015 |
Bio Basic |
10g |
EUR 96.54 |
|
Agarose Gel DNA Extraction and Purification |
EP10010 |
Neuromics |
100 Tests |
EUR 356.4 |
Agarose gel DNA extraction and purification kit |
K1441-100 |
Biovision |
each |
EUR 510 |
Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins) |
110200-NG |
Alpha Diagnostics |
5 ml |
EUR 343.2 |
Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins) |
110100-CG |
Alpha Diagnostics |
5 ml |
EUR 343.2 |
Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies |
KLH11-G |
Alpha Diagnostics |
1 ml |
EUR 270 |
Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies |
KLH11-G-5 |
Alpha Diagnostics |
5 ml |
EUR 634.8 |
Cys-link agarose gel coupling buffer kit (5-10 peptides) |
110400-CB |
Alpha Diagnostics |
1 kit |
EUR 270 |
GelRED(TM) for Agarose or Page Gels (10000X in water) |
S420 |
GeneOn |
0,5 ml |
EUR 175.2 |
GelRED(TM) for Agarose or Page Gels (10000X in water) |
S425 |
GeneOn |
5x0,5 ml |
EUR 699.6 |
GelRED(TM) for Agarose or Page Gels (10000X in water) |
S425L |
GeneOn |
15x0,5 ml |
Ask for price |
GelRed Agarose LE |
41029-50G |
Biotium |
50G |
EUR 384 |
Description: Minimum order quantity: 1 unit of 50G |
GelRed Agarose LE |
41029-5G |
Biotium |
5G |
EUR 124.8 |
Description: Minimum order quantity: 1 unit of 5G |
GelGreen Agarose LE |
41030-50G |
Biotium |
50G |
EUR 384 |
Description: Minimum order quantity: 1 unit of 50G |
GelGreen Agarose LE |
41030-5G |
Biotium |
5G |
EUR 124.8 |
Description: Minimum order quantity: 1 unit of 5G |
Precast Agarose Gel TBE, 2%, 12 Well, EtBr Stained, 20 gels per Box |
TBE12-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TBE, 2%, 17 Well, EtBr Stained, 20 gels per Box |
TBE17-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TBE, 2%, 48 Well, EtBr Stained, 10 gels per Box |
TBE48-2PER-EB |
Bio Basic |
1BOX, 10UNIT |
EUR 331.44 |
|
Precast Agarose Gel TAE, 2%, 12 Well, EtBr Stained, 20 gels per Box |
TAE12-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TAE, 2%, 17 Well, EtBr Stained, 20 gels per Box |
TAE17-2PER-EB |
Bio Basic |
1BOX, 20UNIT |
EUR 280.8 |
|
Precast Agarose Gel TAE, 2%, 48 Well, EtBr Stained, 10 gels per Box |
TAE48-2PER-EB |
Bio Basic |
1BOX, 10UNIT |
EUR 331.44 |
|
Agarose |
abx082006-100g |
Abbexa |
100 g |
EUR 292.8 |
|
Agarose |
abx082007-100g |
Abbexa |
100 g |
EUR 309.6 |
|
Agarose |
abx082008-100g |
Abbexa |
100 g |
EUR 276 |
|
Agarose |
G060-1 |
ABM |
100 g |
EUR 211.2 |
Agarose |
G060-2 |
ABM |
500 g |
EUR 442.8 |
DNA Loading (6X) Buffer for Agarose and Acrylamide Gels |
306-205 |
GeneOn |
5x1 ml |
EUR 56.4 |
Precast Agarose Gel TBE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box |
TBE12-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TBE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box |
TBE17-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TBE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box |
TBE48-2PER-SF |
Bio Basic |
1BOX, 10UNIT |
EUR 440.02 |
|
Precast Agarose Gel TAE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box |
TAE12-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TAE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box |
TAE17-2PER-SF |
Bio Basic |
1BOX, 20UNIT |
EUR 343.44 |
|
Precast Agarose Gel TAE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box |
TAE48-2PER-SF |
Bio Basic |
1BOX, 10UNIT |
EUR 440.02 |
|
Agarose LE |
A-1270-100 |
ABTBeads |
100 g |
EUR 139.2 |
Agarose LE |
A-1270-500 |
ABTBeads |
500 g |
EUR 349.2 |
Agarose HR |
A-1280-100 |
ABTBeads |
100 g |
EUR 219.6 |
Agarose HR |
A-1280-500 |
ABTBeads |
500 g |
EUR 822 |
Agarose GA |
A-1290-100 |
ABTBeads |
100 g |
EUR 123.6 |
Agarose GA |
A-1290-500 |
ABTBeads |
500 g |
EUR 306 |
Agarose LM |
A-1300-100 |
ABTBeads |
100 g |
EUR 230.4 |
Agarose LM |
A-1300-25 |
ABTBeads |
25 g |
EUR 123.6 |
Therefore, 14 and 7 potential glycan structures were successfully disclosed, respectively. In total, 15 glycan compositions and 22 potential glycan structures are identified and marked for AZGP1, including several different structures with the same composition. In particular, 5 potential glycan structures are identified as a unique signature of lung cancer. Our strategy developed accommodates the promise for comprehensive identification of the glycan structure on the target biomarker glycoprotein. The deep characterization of its glene structure will ultimately increase its sensitivity and specificity for cancer detection.