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Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis.

Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis.
Posted by Troy

Multi-drug resistant (MDR) Acinetobacter baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify patterns of antibiotic resistance, biofilm formation and clonal relationship of clinical and environmental isolates of A. baumannii by Pulsed Field Gel Electrophoresis method. Forty-three clinical and environmental isolates 26 of MDR A. baumannii were collected and recognized by the API 20NE. antibiotic resistance isolates was assessed by disk diffusion method, and biofilm formation test was conducted using microtiter plate.

Pulsed Field Gel Electrophoresis (PFGE) was used to assess the genomic features of bacterial resistance levels isolates.The clinical and environmental isolates to antibiotics were 95-100%. Differences in levels of antibiotic resistance among clinical and environmental isolates were not statistically significant (p> 0.05). the ability of biofilm production revealed that 31 (44.9%), and 30 (43.5%) isolates had a strong biofilm producer activity and moderate, respectively.

PFGE typing exhibited eight different clusters (A, B, C, D, E, F, G and H) with two significant clusters including A and G with 21 (30.4%) and 16 (23.2%) members each, comprising to 53.6% of all isolates. There is no relationship between biofilm formation and antibiotic resistance pattern with pulsotypes.The PFGE results showed that there is a close link between environmental and clinical isolates of A. baumannii.

Cross-contamination is also very important that occur through daily clinical activities between environmental and clinical isolates. Therefore, in order to minimize contamination of clonal MDR A. baumannii isolates and clinical environment, it is necessary to use strict infection control strategy.

Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis.
Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis.

Size proper separation of silicon quantum dots of red-to-near-infrared-luminescent water soluble by gel electrophoresis.

Gel electrophoresis, which is the standard method for the separation and analysis of macromolecules such as DNA, RNA and protein, applied for the first time on a silicon (Si) quantum dots (QDs) for sizing. In Si QDs studied, boron (B) and phosphorus (P) simultaneously doped. Codoping induces a negative potential on the surface of Si QD and make it dispersible in water. Si QDs with different concentrations of B and P and grow at different temperatures (950 ° C-1200 ° C) was studied.

This indicates that a native polyacrylamide gel electrophoresis to separate the codoped Si QDs by size. Gel electrophoresis ability to paralyze the size of the QDs are separated in a solid matrix made a detailed analysis of the size-purified Si QDs possible. For example, photoluminescence (PL) studies of the dry gel Si QDs grown at 1100 ° C showed that the PL spectra of Si QD solution with a maximum of about 1.4 eV PL can be separated into more than 15 spectrum with a maximum PL change from 1.2 to 1 , 8 eV depending on the distance of migration.

It is found that the relationship between the PL peak energy and migration distance depends on temperature growth of Si QDs, and B and the concentration of P. For all samples with a different impurity concentration and grow at different temperatures, a clear trend was observed in the relationship between the full width at half the maximum (FWHM) and the PL peak energy spectrum in a wide energy range.

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
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electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
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electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

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EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
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EV3620-US ea
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EV1450-UK ea
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EV1450-US ea
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EV2310 ea
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EV2310-UK ea
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EV2310-US ea
EUR 686.4

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EV2650 ea
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EV2650-UK ea
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EV2650-US ea
EUR 686.4

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EV3020-US ea
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CometAssay Electrophoresis Starter Kit

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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
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ELE3400 EACH
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High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

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High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

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Acrylamide, suitable for electrophoresis

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Acrylamide, suitable for electrophoresis

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High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

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ES Electrophoresis Casting Tray, 7cm X 7cm

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Bovine Serum Albumin, 98% by electrophoresis

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Bovine Serum Albumin, 98% by electrophoresis

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Bovine Serum Albumin, 98% by electrophoresis

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ES Electrophoresis Casting Tray, 7cm X 14cm

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Electrophoresis Apparatus - 7x10cm (6-10-well)

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CometAssay Electrophoresis Starter Kit plus EU Power Supply

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4250-050-ESK-PS3 1 Kit
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CometAssay Electrophoresis Starter Kit plus SZ Power Supply

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Accuris myGel Mini Electrophoresis Syst w/ Transf

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Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
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Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
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Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
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10-well protein Vertical Electrophoresis Apparatus

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runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

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SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
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Description: Reagents-for-ssDNA-or-RNA-electrophoresis

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
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runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

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SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
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myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
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myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

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OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

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Agarose Gel Extraction Kit - Column Kit

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Agarose gel DNA extraction and purification kit

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Agarose Gel Loading Dye 6X Alkaline

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Agarose Gel DNA Extraction and Purification

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40120712-1 10x20 mL
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Cys-link agarose gel coupling buffer kit (5-10 peptides)

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40120331-1 25 ml
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Precast Agarose Gel TBE, 2%, 12 Well, EtBr Stained, 20 gels per Box

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Precast Agarose Gel TAE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

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GelRed Agarose LE

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GelRed® Agarose LE

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Agarose

20-abx186369
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The FWHM increases with increasing peak energy and it was almost two times greater than that observed for undoped Si QDs. Large PL FWHM of codoped Si QDs demonstrate that excitons then translated into codoped Si QDs for their droppings charged.

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