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Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Posted by Troy

Various genetic-based approaches including mutant population screens, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. However, this genetic tool is not without a limitation attached. One alternative approach to studying meiosis plants, especially in polypoid such as Triticum Aestivum L. (wheat bread), is proteomic.

However, protein-based approaches using proteomics are rarely explained, with only two efforts to study the initial plant meiosis reported. Here, we report an inquiry of Meiosis wheat early bread using proteomics. Five protein spots that differed differently were identified using 2D gel electrophoresis (2DGE) in protein extract from four stages of Pound Meiosis and three genotypes (Chinese spring wild type, PH1B and PH2A wheat mutants). Tandem Mass Spectrometry (MS / MS / MS) Identification of peptides from these protein spots causes insulation and characterization of full length clones of wheat speckle type Puze proteins, proteins such as SF21 and HSP70, and the order of partial coding.

Hexose transporter. Significantly, the pale function of the protein POZ type Speckle and HSP70 is confirmed using the DNA binder test in vitro. Through the use of a 2DGE proteomic approach, we show that proteomic is a feasible alternative for genetic-based approaches when studying meiosis in wheat. More significant, we report a potential role for speckle type PUZ proteins and HSP70 in chromosome pairs during the early stages of meiosis on wheat bread.

Assessment of genotoxic effects of pendiumphalin in Chinese hamsters over cells with a single cell electrophoresis test (comet)

PendiMethalin (N-ethylpropopyl) -3.4-dimethyl-2,6-dinitrobenzenine) is a dinitroaniline herbicide compound that selectively controls weeds. This is cell division and growth inhibitors. It goes down the plant in a short time after Seedling. This is a soil and water pollutan because of the widespread use of formulations in Turkey and throughout the world. PendiMethalin is produced and imported by Turkey. PendiMethalin is a slightly toxic compound that is classified in class 3 toxicity by the United States Environmental Protection Agency (USPA). Although classified as Group C (human maybe carcinogens) compound by USEPA, there are a number of studies of their genotoxic effects. The purpose of this study was to evaluate vitro genotoxic effects from various methalized concentrations in the top Chinese hamsters (CHO) cells with a single cell gel electrophoresis test (comet).

Materials and Methods: Cells incubated with a concentration of pendinalin 1, 10, 100, 100, 1000 and 10000 μm for 30 minutes at 37 ° C and DNA damage compared to Cho cells that are not treated with pendiMethaline. 50 μm hydrogen peroxide is used as a positive control.

Results: There are no significant cytotoxic effects observed in the concentration range studied. DNA damage in Cho cells increased significantly in the concentration of pendiDalinalin 1, 100, 1000 and 10000 μm, a significant decrease was observed in the concentration of 10 μm pendiumphalization.

The application of capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE) for the separation of human insulin, lispro insulin and their degradation products.

The method of two capillar electrophoresis (CE) has been developed for the separation of cars and mass variants of human insulin and recombinous analogues of Lispro. Because the capillar zone (CZE) and capillar gel electrophoresis (CGE) are based on different separation principles, they can be used to detect different insulin impurities and analogues. The application of CZE allows the separation of compounds with different M / Z ratios, therefore CZE is a suitable method for the separation of Deamidation Insulin products. After optimization, this method is validated according to the requirements of ICH. The CGE method is used for separation of higher molecular weight transformation products. Experimental data shows that CZE and CGE are simple, fast and strong methods that can be used as routine analysis for the quality control of insulin formulation

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics.

Production of high-quality two-dimensional gel electrophoresis profiles for MEDAKA Marine samples using trizol-based protein extraction approaches.

Medaka Marines is one of the most popular species of fish species for ecotoxicology and environmental research and proteomic studies is a tool that is useful for understanding Medaka molecular responses on exposure with environmental stressors. The preparation of high-quality protein samples is the key to producing high-quality two-dimensional electrophoresis gel results (2-DE) for proteomic analysis. In recent years, trizol-based protein extraction has gained popularity because of its promising performance in producing high-quality 2-de and convenience methods.

Three Trizol-Based Approaches (Trizol Methods, Trizol Alikuot Methods and Trizol Methods with Commercial Cleaning Kits) are used to extract proteins from Medaka Marine samples and 2-de profiles produced. 2-de profile quality and effectiveness of extraction methods evaluated. In comparison, two common protein extraction methods (methods of buffer lysis and extraction of trichodic acid / acetone precipitation) are also applied in parallel with a trizol-based approach. Each trizol-based approach produces high-quality 2-de high-quality Marine Profile Medaka compared to the two methods of Buffer Lisis and TCA / Aceton rainfall extraction.

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)
E1101-SK 1 each
EUR 712.8

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)
E1101-SK-E 1 PC
EUR 742.5

Accuris myGel Mini Electrophoresis Syst w/ Transf
ELE1111 EACH
EUR 854.4

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules
VS10WCES EACH
EUR 1434.85

Chiller for Electrophoresis systems
CSLCHILLER EACH
EUR 6793.2

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380
ACT-CBMN-7 each
EUR 892.8

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator
E1201 1 each
EUR 1001.7

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input
E1201-E 1 each
EUR 1058.9

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs
ELE2300 EACH
EUR 1602

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs
ELE2302 EACH
EUR 1681.2

electrophoresis ps 1500v, 300ma, 150w
EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w
EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w
EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w
EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w
EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w
EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w
EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w
EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w
EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w
EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w
EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w
EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w
EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w
EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w
EV3620-US ea
EUR 1456.8

electrophoresis ps 400v, 500ma, 50w
EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w
EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w
EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w
EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w
EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w
EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w
EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w
EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w
EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w
EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w
EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w
EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w
EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w
EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w
EV3610-US ea
EUR 960

electrophoresis power supply
EV245 ea
EUR 574.8

Agarose for DNA electrophoresis
16-0027-500 500 g
EUR 410.4

5 x SDS Electrophoresis Buffer
GR103039 1 L
EUR 107

ES Horizontal Electrophoresis Set
LE1011 Set
EUR 578.4

6X6 well Electrophoresis Apparatus
EDK626 1 UNIT
EUR 750.6

electrophoresis power supply 120 vac
EV245-US ea
EUR 574.8

Starch (Smithies), for electrophoresis
GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis
GE6857-500G 500 g
EUR 217.2

electrophoresis power supply uk plug
EV245-UK ea
EUR 574.8

CometAssay Electrophoresis Starter Kit
4250-050-ESK 1 Kit
EUR 3749.28

Acrylamide, suitable for electrophoresis
GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis
GK5651-100 100
EUR 23.8

ES 6 Tooth Gel Comb For ES Electrophoresis
LE1011-A4 Ea
EUR 148.8

ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2 Ea
EUR 181.2

4 x Phosphate/SDS Electrophoresis Buffer
GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis
GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis
GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis
GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis
GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis
GE3699-500 500
EUR 506.2

ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1 Ea
EUR 198

ES 8/10 Double Gel Comb For ES Electrophoresis
LE1011-A3 Ea
EUR 148.8

Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613 1 UNIT
EUR 487.51

CometAssay Single Cell Gel Electrophoresis Assay
4250-050-K 50 tests
EUR 513.6

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels
LE1001 Set
EUR 651.6

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels
LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels
LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels
LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels
LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels
LE1006 Set
EUR 1170

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)
EDK614 1 UNIT
EUR 520.92

10-well protein Vertical Electrophoresis Apparatus
EDK692 1 UNIT
EUR 487.51

CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K 1 kit
EUR 684.96

CometAssay Electrophoresis Starter Kit plus EU Power Supply
4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply
4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply
4250-050-ESK-PS4 1 Kit
EUR 4597.68

Electro blot Mini system 10x10cm
EBM10 EACH
EUR 970.8

Complete OmniPAGE Mini system with electroblotting module
CVS10CBS EACH
EUR 928.28

mini system 7x8 cm
EHS3100-SYS ea
EUR 524.4

mini system 8x10 cm
EHS1050-SYS ea
EUR 422.4

Glass tubes 24/150mm for Electrothermal STEM Integrity 10 system
HEA5636 PK10
EUR 298.8

Stirrer bars 10/6mm for Electrothermal STEM Integrity 10 system
HEA5638 PK10
EUR 60

Stirrer bars 12/4.5mm for Electrothermal STEM Integrity 10 system
HEA5640 PK10
EUR 60

Thermocouple probe for Electrothermal STEM Integrity 10 system
HEA5644 PK6
EUR 1006.8

Integrity software for Electrothermal STEM Integrity 10 system
HEA5646 EACH
EUR 4102.8

Multi-Temp10 Module for Electrothermal STEM Integrity 10 system
HEA5642 EACH
EUR 2816.4

mini vertical system 10x10 cm
EVS1100-SYS ea
EUR 727.2

mini vertical system 20x10 cm
EVS1200-SYS ea
EUR 1030.8

Non-intrusive IR sensor for Electrothermal STEM Integrity 10 system
HEA5648 EACH
EUR 1869.6

Intrusive IR probe in Hastelloy for Electrothermal STEM Integrity 10 system
HEA5652 EACH
EUR 4159.86

PTFE cap with valve and sensor inlet for Electrothermal STEM Integrity 10 system
HEA5634 PK10
EUR 2377.2

SmartSEC™ Mini EV Isolation System
SSEC100A-1 10 rxn
EUR 391

Intrusive IT probe in stainless steel for Electrothermal STEM Integrity 10 system
HEA5650 EACH
EUR 4986

IsoPure™ Mini, Automated Purification System, 115V
AP1016 1 each
EUR 10447.5

IsoPure™ Mini, Automated Purification System, 230V
AP1016-E 1 each
EUR 10447.5

Multi Sub Mini Negative Electrode
MS7NE EACH
EUR 114

Multi Sub Mini Positive Electrode
MS7PE EACH
EUR 114

MiniVac Waste Aspiration System
ARG1464 EACH
EUR 326.4

KNF Laboport SH 820 G Vacuum pump system 20L/min
PUM1180 EACH
EUR 3033.6

KNF Laboport SR 820 G Vacuum pump system 20L/min
PUM1182 EACH
EUR 2617.2

KNF Laboport SH 840 G Vacuum pump system 34L/min
PUM1184 EACH
EUR 3427.2

KNF Laboport SR 840 G Vacuum pump system 34L/min
PUM1186 EACH
EUR 3025.2

Welch MXPC 303 Z Laboratory Vacuum System 33L/min 5mbar
PUM1210 EACH
EUR 2976

Welch MXPC 603 T Laboratory Vacuum System 65L/min 2mbar
PUM1212 EACH
EUR 4490.4

New system
VS10WPGS2 EACH
EUR 94.8

Common diseases of the cardiovascular system, 23 cases (2mm)
CVD231 1
EUR 228
Description: Cardiovascular disease tissue array, non-overlapping with CVD481, 23 cases of normal, reactive and neoplastic conditions of the heart and blood vessels. Designed for blood vessel or endothelial cell related studies.

SEALING MAT, FOR 1.0 ML 96 DEEP WELL PLATES, ROUND WELLS, AND AXYGEN MINI TUBE SYSTEM, SILICONE, NATURAL, NONSTERILE, BULK
AM-750UL-RD 10/pk
EUR 196.8
Description: Sealing Products; Sealing mats - Axygen

ProtoBlock System
NAT1004 1KIT
EUR 93.6

SS grid electropolished for size 30
INC8202 EACH
EUR 133.2

Megaprime Dna Labelling System Dctp 30Rxn
RPN1606 EACH
EUR 846

Megaprime Dna Labelling System Dctp 60Rxn
RPN1607 EACH
EUR 1126.8

SS grid electropolished for sizes 260/400
CHA4052 EACH
EUR 224.4

CCD cooling system
3600100 1unit
EUR 5896.8

SS grid electropolished for HPP750
CHA4054 EACH
EUR 318

Nalgene System 100 Cryobox
CRY8305 PK10
EUR 211.2

AEC coloring system
CT356 10-plate
EUR 169.2

Watershed System Kit
M-WS180320-1 1 KIT
EUR 9296
Description: Watershed System Kit

Lower cooling system
WAT4796 EACH
EUR 1310.82

Campact Vacuum System
AC701 EACH
EUR 2665.2

Disposable Bag System
H20CBS50 EACH
EUR 174

Electroporation Cuvettes 1mm Long
FBR-201 PK50
EUR 134.4

Electroporation Cuvettes 2mm Long
FBR-202 PK50
EUR 132

Electroporation Cuvettes 4mm Long
FBR-204 PK50
EUR 134.4

Electroporation Cuvettes 1mm Short
FBR-101 PK50
EUR 132

Electroporation Cuvettes 2mm Short
FBR-102 PK50
EUR 130.8

Fast Mutagenesis System
20-abx098073
  • EUR 610.80
  • EUR 710.40
  • 10 rxns
  • 20 rxns

Cometslides Rack System
4252-040-02 1 Each
EUR 256.56

data acquisition system
D230 ea
EUR 2044.8

Extractor System Option
DRY1128 EACH
EUR 594.05

Propane Transfer System
M-CEM-37015 1 UNIT
EUR 688
Description: Propane Transfer System

MBP electroporator cuvettes 2 mm gap size - blue
DD38190 PK50
EUR 300

Heidolph clamping system
509-63100-00 EACH
EUR 169.73

Scienceware protective lab-labeling system tape
Z115509-1EA EACH
EUR 57.36

Ez C02 System Sachets
260679 PK20
EUR 157.98

Dual Spot PLUS System
MIC2516 EACH
EUR 1183.2

AFS-EDI SYSTEM CART
ZAFSCART2 EACH
EUR 1065.37

Digestive system (esophagus
GI1441 each
EUR 306
Description: Digestive system (esophagus, stomach, colon, rectum, liver, pancreas) cancer with matched adjacent normal tissue array, including TNM, pathology grade and clinical stage, 72 cases/144 cores, replaced by GI1441a

Digestive system (esophagus
GI701a each
EUR 222
Description: Digestive system (esophagus, stomach, colon, rectum, liver, pancreas) cancer with matched adjacent normal tissue array, including TNM, pathology grade and clinical stage, 35 cases/70 cores, replacing GI701

photo documentation system
VLBPCX4-20M ea
EUR 7622.4

photo documentation system
VLBPCX4-20MX ea
EUR 8128.8

photo documentation system
VLDP-HOOD ea
EUR 6100.8

photo documentation system
VLDP17-26M ea
EUR 9751.2

photo documentation system
VLDP17-26MX ea
EUR 10156.8

Syngene GBox F3 System
ELE7580 EACH
EUR 9476.4

data acquisition system 120 vac
D230-US ea
EUR 2044.8

Ez Campy System Sachets
260680 PK20
EUR 75.6

modular system 10x10 cm
EVS1100-MULTI ea
EUR 1436.4

modular system 20x10 cm
EVS1200-MULTI ea
EUR 1741.2

modular system 20x20 cm
EVS1300-MULTI ea
EUR 2349.6

96-Well Vacuum System
SD5011 1 UNIT
EUR 388.86

Elevator for Osmomat auto with cooling system
WAT4798 EACH
EUR 1693.36

Ez Anaerobe Pouch System
260683 PK20
EUR 147.53

vertical system 10x10 cm
EVS3100-SYS ea
EUR 930

vertical system 20x10 cm
EVS3200-SYS ea
EUR 1741.2

vertical system 20x20 cm
EVS3300-SYS ea
EUR 2044.8

KNF SC920G Vacuum System
PUM5198 EACH
EUR 5254.8

PiranhaTM Electroporation-ready mRNA (10µg)
PTPD500A-1 10ug
EUR 308

BCIP/NBT Substrate System
GR103022 2 x 25 mL
EUR 119

Connector 5m System-POD
ZFC0NN5SQ EACH
EUR 181.48

sequencing system 20x50 cm
ESEQ1200-SYS ea
EUR 1842

Foot Pedal Complete System
FLY1234 EACH
EUR 1056.23

Syngene InGenius 3 System
ELE7540 EACH
EUR 4290

Vials Cryogenic 1 Ml System 100
5000-1012 PK500
EUR 644.4

SYSTEM STAND, AFS-3/3D
ZAF3STAND EACH
EUR 720.69

BD600 BOD System for 6
WAT2030 EACH
EUR 2805.6

BD606 BOD System for 12
WAT2032 EACH
EUR 4584

Slidefile Storage System 100 Positions
M700-100W-WHITE 1 Case(s)
EUR 230.4

Plant Electroporation Buffer for Transfection
GR103051 1 L
EUR 119

Digestive system tissue array
DGS2081 each
EUR 474
Description: Digestive system tissue array, including TNM, stage and grade, 104 cases/208 cores

EpiSonic 2000 Sonication System
EQC-2000-1 1 unit
EUR 20590.9

EasyDip Slide Staining System
M900-12AS-ASSORTED 1 Case(s)
EUR 211.2

HRP Floresce Substrate System
GR103054 Each
EUR 83

Syngene DigiGenius System DG1
ELE7500 EACH
EUR 2697.6

Amersham Rediprime II DNA Labeling System 30 reactions
RPN1633 EACH
EUR 740.4

Gel Doc System EtBr Filter
ELE2500 EACH
EUR 7088.4

VeriClean Triple Bucket System
CLE9830 EACH
EUR 1866

GeneGnome XRQ 16 bit system
ELE2116 EACH
EUR 10857.6

Axygen Gel Documentation System
GD-1000 EACH
EUR 5947.38

AP Fluorescent Substrate System
GR103053 Each
EUR 83

RIOS(TM) 120 SYSTEM 230V 50/60 HZ (LC)
ZR0L51120 EACH
EUR 20546.23

RIOS(TM) 120 SYSTEM 230V 50/60 HZ (HC)
ZR0L52120 EACH
EUR 22808.83

Nugenius Gel Docu System LED
ELE0028 EACH
EUR 7538.4

Safevac Vacuum Aspiration System
SLS4950 EACH
EUR 2056.32

maxi vertical system 20x20 cm
EVS1300-SYS ea
EUR 1365.6

Nugenius Gel Docu System Plus
ELE0030 EACH
EUR 9004.8

Fast MultiSite Mutagenesis System
abx098861-10rxns 10 rxns
EUR 828

UreaGel 29:1 System 1L Kit
NAT1128 EACH
EUR 166.8

UreaGel 29:1 System 2.2L Kit
NAT1130 EACH
EUR 283.2

PiranhaTM Electroporation-ready RFP-tagged mRNA (10µg)
PTPD510A-1 10ug
EUR 339

PiranhaTM Electroporation-ready GFP-tagged mRNA (10µg)
PTPD520A-1 10ug
EUR 339

Episomal iPSC Reprogramming System
SC900A-1 5 reactions
EUR 1161.6

P-Vac Portable Vacuum System
ARG1608 EACH
EUR 1075.81

Kinesis Luertight System 1/16
CHR4559 EACH
EUR 25.2

RIOS(TM) 60 SYSTEM 230V 50/60 HZ (LC)
ZR0L51060 EACH
EUR 17612.54

RIOS(TM) 60 SYSTEM 230V 50/60 HZ (HC)
ZR0L52060 EACH
EUR 19531.78

In addition, the Trizol method with a commercial cleaning kit produces the best 2-daut profiles in terms of background clarity, the number of spots and resolution of the Proteins-based approach. Trizol offers better choices than traditional protein extraction methods for 2-de analysis. Medaka Marines. The modified version of the Trizol method with a commercial cleaning kit is proven to produce the best 2-de profile.

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