Various genetic-based approaches including mutant population screens, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. However, this genetic tool is not without a limitation attached. One alternative approach to studying meiosis plants, especially in polypoid such as Triticum Aestivum L. (wheat bread), is proteomic.

However, protein-based approaches using proteomics are rarely explained, with only two efforts to study the initial plant meiosis reported. Here, we report an inquiry of Meiosis wheat early bread using proteomics. Five protein spots that differed differently were identified using 2D gel electrophoresis (2DGE) in protein extract from four stages of Pound Meiosis and three genotypes (Chinese spring wild type, PH1B and PH2A wheat mutants). Tandem Mass Spectrometry (MS / MS / MS) Identification of peptides from these protein spots causes insulation and characterization of full length clones of wheat speckle type Puze proteins, proteins such as SF21 and HSP70, and the order of partial coding.

Hexose transporter. Significantly, the pale function of the protein POZ type Speckle and HSP70 is confirmed using the DNA binder test in vitro. Through the use of a 2DGE proteomic approach, we show that proteomic is a feasible alternative for genetic-based approaches when studying meiosis in wheat. More significant, we report a potential role for speckle type PUZ proteins and HSP70 in chromosome pairs during the early stages of meiosis on wheat bread.

Assessment of genotoxic effects of pendiumphalin in Chinese hamsters over cells with a single cell electrophoresis test (comet)

PendiMethalin (N-ethylpropopyl) -3.4-dimethyl-2,6-dinitrobenzenine) is a dinitroaniline herbicide compound that selectively controls weeds. This is cell division and growth inhibitors. It goes down the plant in a short time after Seedling. This is a soil and water pollutan because of the widespread use of formulations in Turkey and throughout the world. PendiMethalin is produced and imported by Turkey. PendiMethalin is a slightly toxic compound that is classified in class 3 toxicity by the United States Environmental Protection Agency (USPA). Although classified as Group C (human maybe carcinogens) compound by USEPA, there are a number of studies of their genotoxic effects. The purpose of this study was to evaluate vitro genotoxic effects from various methalized concentrations in the top Chinese hamsters (CHO) cells with a single cell gel electrophoresis test (comet).

Materials and Methods: Cells incubated with a concentration of pendinalin 1, 10, 100, 100, 1000 and 10000 μm for 30 minutes at 37 ° C and DNA damage compared to Cho cells that are not treated with pendiMethaline. 50 μm hydrogen peroxide is used as a positive control.

Results: There are no significant cytotoxic effects observed in the concentration range studied. DNA damage in Cho cells increased significantly in the concentration of pendiDalinalin 1, 100, 1000 and 10000 μm, a significant decrease was observed in the concentration of 10 μm pendiumphalization.

The application of capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE) for the separation of human insulin, lispro insulin and their degradation products.

The method of two capillar electrophoresis (CE) has been developed for the separation of cars and mass variants of human insulin and recombinous analogues of Lispro. Because the capillar zone (CZE) and capillar gel electrophoresis (CGE) are based on different separation principles, they can be used to detect different insulin impurities and analogues. The application of CZE allows the separation of compounds with different M / Z ratios, therefore CZE is a suitable method for the separation of Deamidation Insulin products. After optimization, this method is validated according to the requirements of ICH. The CGE method is used for separation of higher molecular weight transformation products. Experimental data shows that CZE and CGE are simple, fast and strong methods that can be used as routine analysis for the quality control of insulin formulation

.

Production of high-quality two-dimensional gel electrophoresis profiles for MEDAKA Marine samples using trizol-based protein extraction approaches.

Medaka Marines is one of the most popular species of fish species for ecotoxicology and environmental research and proteomic studies is a tool that is useful for understanding Medaka molecular responses on exposure with environmental stressors. The preparation of high-quality protein samples is the key to producing high-quality two-dimensional electrophoresis gel results (2-DE) for proteomic analysis. In recent years, trizol-based protein extraction has gained popularity because of its promising performance in producing high-quality 2-de and convenience methods.

Three Trizol-Based Approaches (Trizol Methods, Trizol Alikuot Methods and Trizol Methods with Commercial Cleaning Kits) are used to extract proteins from Medaka Marine samples and 2-de profiles produced. 2-de profile quality and effectiveness of extraction methods evaluated. In comparison, two common protein extraction methods (methods of buffer lysis and extraction of trichodic acid / acetone precipitation) are also applied in parallel with a trizol-based approach. Each trizol-based approach produces high-quality 2-de high-quality Marine Profile Medaka compared to the two methods of Buffer Lisis and TCA / Aceton rainfall extraction.

mini system 7x8 cm
EHS3100-SYS	Consort	ea	EUR 437

mini vertical system 10x10 cm
EVS1100-SYS	Consort	ea	EUR 606

mini vertical system 20x10 cm
EVS1200-SYS	Consort	ea	EUR 859

CYFRA211 (Keratin 21-1) ELISA test
23	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CYFRA211 (Keratin 21-1)

electrophoresis power supply
EV245	Consort	ea	EUR 479

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit
MEA384Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 3645.03
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit
MEA384Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 387.83
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit
MEA384Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 511.18
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit
MEA384Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2000.31
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit
4-MEA384Mu	Cloud-Clone	EUR 3696.00 EUR 1951.00 EUR 512.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Interleukin 23 (IL23) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit
MEA384Ra-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5523.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit
MEA384Ra-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 542.52
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit
MEA384Ra-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 732.17
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit
MEA384Ra-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2994.77
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit
4-MEA384Ra	Cloud-Clone	EUR 5574.00 EUR 2945.00 EUR 733.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Rat Interleukin 23 (IL23) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Mini Samples Interleukin 23 ELISA Kit (IL23)
RK02941	Abclonal	96 Tests	EUR 521

Common diseases of the cardiovascular system, 23 cases (2mm)
CVD231	Pantomics	1	EUR 190
Description: Cardiovascular disease tissue array, non-overlapping with CVD481, 23 cases of normal, reactive and neoplastic conditions of the heart and blood vessels. Designed for blood vessel or endothelial cell related studies.

6X6 well Electrophoresis Apparatus
EDK626	Bio Basic	1 UNIT	EUR 625.5

Starch (Smithies), for electrophoresis
GE6857-1KG	Glentham Life Sciences	1 kg	EUR 309

Starch (Smithies), for electrophoresis
GE6857-500G	Glentham Life Sciences	500 g	EUR 181

Acrylamide, suitable for electrophoresis
GK5651-100G	Glentham Life Sciences	100 g	EUR 54

ES Horizontal Electrophoresis Set
LE1011	GenDepot	Set	EUR 482

Agarose for DNA electrophoresis
16-0027-500	Medicago	500 g	EUR 342

CometAssay Electrophoresis Starter Kit
4250-050-ESK	Biotechne	1 Kit	EUR 3124.4

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit
MEA746Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5242.01
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit
MEA746Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 519.34
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit
MEA746Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 699.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit
MEA746Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2845.77
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit
4-MEA746Mu	Cloud-Clone	EUR 5293.00 EUR 2796.00 EUR 700.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) in samples from n/a with no significant corss-reactivity with analogues from other species.

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380
ACT-CBMN-7	ACTGene		EUR 744

electrophoresis ps 400v, 500ma, 50w
EV1450	Consort	ea	EUR 479

electrophoresis ps 400v, 500ma, 50w
EV1450-UK	Consort	ea	EUR 479

electrophoresis ps 400v, 500ma, 50w
EV1450-US	Consort	ea	EUR 479

electrophoresis ps 1500v, 300ma, 150w
EV2230	Consort	ea	EUR 749

electrophoresis ps 1500v, 300ma, 150w
EV2230-UK	Consort	ea	EUR 749

electrophoresis ps 1500v, 300ma, 150w
EV2230-US	Consort	ea	EUR 749

electrophoresis ps 300v, 1000ma, 150w
EV2310	Consort	ea	EUR 572

electrophoresis ps 300v, 1000ma, 150w
EV2310-UK	Consort	ea	EUR 572

electrophoresis ps 300v, 1000ma, 150w
EV2310-US	Consort	ea	EUR 572

electrophoresis ps 3000v, 150ma, 150w
EV2320	Consort	ea	EUR 749

electrophoresis ps 3000v, 150ma, 150w
EV2320-UK	Consort	ea	EUR 749

electrophoresis ps 3000v, 150ma, 150w
EV2320-US	Consort	ea	EUR 749

electrophoresis power supply uk plug
EV245-UK	Consort	ea	EUR 479

electrophoresis power supply 120 vac
EV245-US	Consort	ea	EUR 479

electrophoresis ps 600v, 500ma, 150w
EV2650	Consort	ea	EUR 572

electrophoresis ps 600v, 500ma, 150w
EV2650-UK	Consort	ea	EUR 572

electrophoresis ps 600v, 500ma, 150w
EV2650-US	Consort	ea	EUR 572

electrophoresis ps 300v, 2000ma, 300w
EV3020	Consort	ea	EUR 800

electrophoresis ps 300v, 2000ma, 300w
EV3020-UK	Consort	ea	EUR 800

electrophoresis ps 300v, 2000ma, 300w
EV3020-US	Consort	ea	EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150	Consort	ea	EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150-UK	Consort	ea	EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150-US	Consort	ea	EUR 800

electrophoresis ps 3000v, 300ma, 300w
EV3330	Consort	ea	EUR 1214

electrophoresis ps 3000v, 300ma, 300w
EV3330-UK	Consort	ea	EUR 1214

electrophoresis ps 3000v, 300ma, 300w
EV3330-US	Consort	ea	EUR 1214

electrophoresis ps 600v, 1000ma, 300w
EV3610	Consort	ea	EUR 800

electrophoresis ps 600v, 1000ma, 300w
EV3610-UK	Consort	ea	EUR 800

electrophoresis ps 600v, 1000ma, 300w
EV3610-US	Consort	ea	EUR 800

electrophoresis ps 6000v, 150ma, 300w
EV3620	Consort	ea	EUR 1214

electrophoresis ps 6000v, 150ma, 300w
EV3620-UK	Consort	ea	EUR 1214

electrophoresis ps 6000v, 150ma, 300w
EV3620-US	Consort	ea	EUR 1214

Anti-Dimethyl Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone#RM181
M06819-23	BosterBio	100ug	EUR 468
Description: Anti-Dimethyl Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone#RM181 tested in WB, ELISA, Multiplex, ChIP, IHC, reactive to All Vertebrates

IL-12, Interleukin-12, human
RC212-23	Bio Basic	2ug	EUR 104.38

Endostatin, human
RC214-23	Bio Basic	20ug	EUR 104.38

Betacellulin, human
RC218-23	Bio Basic	5ug	EUR 104.38

S100B, monkey
RC230-23	Bio Basic	2ug	EUR 104.38

Betacellulin, murine (mouse)
RC238-23	Bio Basic	5ug	EUR 104.38

CCL12, murine (mouse)
RC335-23	Bio Basic	5ug	EUR 101.33

UBC12, Ubiquitin Conjugating Enzyme 12 (His6-tagged), human
RC612-23	Bio Basic	50ug	EUR 169.63

Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611	Bio Basic	1 UNIT	EUR 319.26

Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613	Bio Basic	1 UNIT	EUR 406.26

10-well protein Vertical Electrophoresis Apparatus
EDK692	Bio Basic	1 UNIT	EUR 406.26

CometAssay Single Cell Gel Electrophoresis Assay
4250-050-K	Biotechne	50 tests	EUR 428

ProtoBlock System
NAT1004	National Diagnostics	1KIT	EUR 102

Interleukin-23/IL-23
E21-S31	EnoGene	10ug	EUR 343

mini-ANP
5-01547	CHI Scientific	4 x 1mg	Ask for price

Mini Centrifuge
CTFG	BioAssay Systems	1 unit	EUR 89
Description: Small Centrifuge used for preparation of fish and wine samples for use with the QuantiQuikâ„¢ Histamine Quick Test Strips .

Mini BlotBoy
B3D1008-E	Benchmark Scientific	1 PC	EUR 437.43

Roto-Mini
R2020-E	Benchmark Scientific	1 PC	EUR 391.18

Roto-Mini
R2024-E	Benchmark Scientific	1 PC	EUR 499.13

ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1	GenDepot	Ea	EUR 165

ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2	GenDepot	Ea	EUR 151

CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K	Biotechne	1 kit	EUR 570.8

Claudin 23 (Claudin 23) Antibody
abx231737-100ug	Abbexa	100 ug	EUR 481

data acquisition system
D230	Consort	ea	EUR 1704

photo documentation system
VLBPCX4-20M	Consort	ea	EUR 6352

photo documentation system
VLBPCX4-20MX	Consort	ea	EUR 6774

photo documentation system
VLDP-HOOD	Consort	ea	EUR 5084

photo documentation system
VLDP17-26M	Consort	ea	EUR 8126

photo documentation system
VLDP17-26MX	Consort	ea	EUR 8464

Fast Mutagenesis System
20-abx098073	Abbexa	EUR 509.00 EUR 592.00	10 rxns 20 rxns

CCD cooling system
3600100	Atto	1unit	EUR 4914

Cometslides Rack System
4252-040-02	Biotechne	1 Each	EUR 213.8

In addition, the Trizol method with a commercial cleaning kit produces the best 2-daut profiles in terms of background clarity, the number of spots and resolution of the Proteins-based approach. Trizol offers better choices than traditional protein extraction methods for 2-de analysis. Medaka Marines. The modified version of the Trizol method with a commercial cleaning kit is proven to produce the best 2-de profile.