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Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Posted by Troy

Various genetic-based approaches including mutant population screens, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. However, this genetic tool is not without a limitation attached. One alternative approach to studying meiosis plants, especially in polypoid such as Triticum Aestivum L. (wheat bread), is proteomic.

However, protein-based approaches using proteomics are rarely explained, with only two efforts to study the initial plant meiosis reported. Here, we report an inquiry of Meiosis wheat early bread using proteomics. Five protein spots that differed differently were identified using 2D gel electrophoresis (2DGE) in protein extract from four stages of Pound Meiosis and three genotypes (Chinese spring wild type, PH1B and PH2A wheat mutants). Tandem Mass Spectrometry (MS / MS / MS) Identification of peptides from these protein spots causes insulation and characterization of full length clones of wheat speckle type Puze proteins, proteins such as SF21 and HSP70, and the order of partial coding.

Hexose transporter. Significantly, the pale function of the protein POZ type Speckle and HSP70 is confirmed using the DNA binder test in vitro. Through the use of a 2DGE proteomic approach, we show that proteomic is a feasible alternative for genetic-based approaches when studying meiosis in wheat. More significant, we report a potential role for speckle type PUZ proteins and HSP70 in chromosome pairs during the early stages of meiosis on wheat bread.

Assessment of genotoxic effects of pendiumphalin in Chinese hamsters over cells with a single cell electrophoresis test (comet)

PendiMethalin (N-ethylpropopyl) -3.4-dimethyl-2,6-dinitrobenzenine) is a dinitroaniline herbicide compound that selectively controls weeds. This is cell division and growth inhibitors. It goes down the plant in a short time after Seedling. This is a soil and water pollutan because of the widespread use of formulations in Turkey and throughout the world. PendiMethalin is produced and imported by Turkey. PendiMethalin is a slightly toxic compound that is classified in class 3 toxicity by the United States Environmental Protection Agency (USPA). Although classified as Group C (human maybe carcinogens) compound by USEPA, there are a number of studies of their genotoxic effects. The purpose of this study was to evaluate vitro genotoxic effects from various methalized concentrations in the top Chinese hamsters (CHO) cells with a single cell gel electrophoresis test (comet).

Materials and Methods: Cells incubated with a concentration of pendinalin 1, 10, 100, 100, 1000 and 10000 μm for 30 minutes at 37 ° C and DNA damage compared to Cho cells that are not treated with pendiMethaline. 50 μm hydrogen peroxide is used as a positive control.

Results: There are no significant cytotoxic effects observed in the concentration range studied. DNA damage in Cho cells increased significantly in the concentration of pendiDalinalin 1, 100, 1000 and 10000 μm, a significant decrease was observed in the concentration of 10 μm pendiumphalization.

The application of capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE) for the separation of human insulin, lispro insulin and their degradation products.

The method of two capillar electrophoresis (CE) has been developed for the separation of cars and mass variants of human insulin and recombinous analogues of Lispro. Because the capillar zone (CZE) and capillar gel electrophoresis (CGE) are based on different separation principles, they can be used to detect different insulin impurities and analogues. The application of CZE allows the separation of compounds with different M / Z ratios, therefore CZE is a suitable method for the separation of Deamidation Insulin products. After optimization, this method is validated according to the requirements of ICH. The CGE method is used for separation of higher molecular weight transformation products. Experimental data shows that CZE and CGE are simple, fast and strong methods that can be used as routine analysis for the quality control of insulin formulation

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics.

Production of high-quality two-dimensional gel electrophoresis profiles for MEDAKA Marine samples using trizol-based protein extraction approaches.

Medaka Marines is one of the most popular species of fish species for ecotoxicology and environmental research and proteomic studies is a tool that is useful for understanding Medaka molecular responses on exposure with environmental stressors. The preparation of high-quality protein samples is the key to producing high-quality two-dimensional electrophoresis gel results (2-DE) for proteomic analysis. In recent years, trizol-based protein extraction has gained popularity because of its promising performance in producing high-quality 2-de and convenience methods.

Three Trizol-Based Approaches (Trizol Methods, Trizol Alikuot Methods and Trizol Methods with Commercial Cleaning Kits) are used to extract proteins from Medaka Marine samples and 2-de profiles produced. 2-de profile quality and effectiveness of extraction methods evaluated. In comparison, two common protein extraction methods (methods of buffer lysis and extraction of trichodic acid / acetone precipitation) are also applied in parallel with a trizol-based approach. Each trizol-based approach produces high-quality 2-de high-quality Marine Profile Medaka compared to the two methods of Buffer Lisis and TCA / Aceton rainfall extraction.

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 727.06

Cellulose Acetate Electrophoresis System

LA1027-1NO 1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System

WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

LA1070-1NO 1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 757.35

ComboMiniTM Horizontal Electrophoresis System (110V)

CBMN-7 each
EUR 500

ComboMiniTM Horizontal Electrophoresis System (220V)

CBMN-8 each
EUR 530

Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH

ELE1111 EACH
EUR 961.2

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH

VS10WCES EACH
EUR 1614.21

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

Chiller for Electrophoresis systems - EACH

CSLCHILLER EACH
EUR 7642.35

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

Electrophoresis baffle for Life mini tank

HTDB03 pcs
EUR 5.6

Hi-Eco Mini Horizontal Electrophoresis K

LA851-1NO 1 unit
EUR 205.56
Description: Hi-Eco Mini Horizontal Electrophoresis K

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

WSHT-miniPRO4 Mini Vertical Electrophoresis Cell

HTES1009 set
EUR 795.2

WSHT-miniPRO2 Mini Vertical Electrophoresis Cell

HTES1010 set
EUR 516.6

WSHT-easyPRO4 Mini Vertical Electrophoresis Cell

HTES1011 set
EUR 837.2

WSHT-easyPRO2 Mini Vertical Electrophoresis Cell

HTES1012 set
EUR 544.6

WSHT-miniDNA Mini Horizontal Electrophoresis Cell

HTES1008 set
EUR 193.2

Electrophoresis baffle for Bio-Rad Mini-PROTEAN

HTDB01 pcs
EUR 2.1

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell

HTES2001-P set
EUR 459.2

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH

ELE2300 EACH
EUR 1802.25

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH

ELE2302 EACH
EUR 1891.35

Electrophoresis Module for Eco-Mini, for 2 gels

AJ846-017-175 each
EUR 604.95

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster)

HTES2001 set
EUR 322

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

ELECTROPHORESIS PS 1200V, 500MA, 300W

EV3150 ea
EUR 1060

ELECTROPHORESIS PS 1200V, 500MA, 300W

EV3150-UK ea
EUR 1060

ELECTROPHORESIS PS 3000V, 200MA, 300W

EV3330 ea
EUR 1600

ELECTROPHORESIS PS 3000V, 200MA, 300W

EV3330-UK ea
EUR 1600

ELECTROPHORESIS PS 6000V, 100MA, 300W

EV3620 ea
EUR 1600

ELECTROPHORESIS PS 6000V, 100MA, 300W

EV3620-UK ea
EUR 1600

Electrophoresis Buffer (10×)

E-BC-R331-100mL 100mL
EUR 15

Electrophoresis Buffer (10×)

E-BC-R331-200mL 200mL
EUR 20

Electrophoresis Buffer (10×)

E-BC-R331-500mL 500mL
EUR 25

Electrophoresis Buffer (10×)

E-BC-R331-each each Ask for price

Electrophoresis Buffer (10x)

MBS2557002-100mL 100mL
EUR 85

Electrophoresis Buffer (10x)

MBS2557002-200mL 200mL
EUR 90

Electrophoresis Buffer (10x)

MBS2557002-500mL 500mL
EUR 95

Electrophoresis Buffer (10x)

MBS2557002-5x500mL 5x500mL
EUR 380

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

ELECTROPHORESIS PS 300V, 1000MA, 150W

EV2310 ea
EUR 750

ELECTROPHORESIS PS 300V, 1000MA, 150W

EV2310-UK ea
EUR 750

ELECTROPHORESIS PS 600V, 500MA, 150W

EV2650 ea
EUR 750

ELECTROPHORESIS PS 600V, 500MA, 150W

EV2650-UK ea
EUR 750

ELECTROPHORESIS PS 300V, 2000MA, 300W

EV3020 ea
EUR 1060

ELECTROPHORESIS PS 300V, 2000MA, 300W

EV3020-UK ea
EUR 1060

ELECTROPHORESIS PS 600V, 1000MA, 300W

EV3610 ea
EUR 1060

ELECTROPHORESIS PS 600V, 1000MA, 300W

EV3610-UK ea
EUR 1060

TEMED, electrophoresis grade

RM10358-100G 1 unit
EUR 301.18
Description: TEMED, electrophoresis grade

electrophoresis power supply

EV245 ea
EUR 574.8

Electrophoresis Power Supply

LA690-1NO 1 unit
EUR 432.84
Description: Electrophoresis Power Supply

Glycine for electrophoresis, 99.5%

52574 100 Gms
EUR 9.58
Description: Part A

ELECTROPHORESIS PS 1200V, 500MA, 300W 120 VAC

EV3150-US ea
EUR 1060

ELECTROPHORESIS PS 3000V, 200MA, 300W 120 VAC

EV3330-US ea
EUR 1600

ELECTROPHORESIS PS 6000V, 100MA, 300W 120 VAC

EV3620-US ea
EUR 1600

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

Agarose Gel Electrophoresis Kit

MB570-10PR 1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit

10X MOPS Electrophoresis Buffer

ML050-500ML 1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer

ELECTROPHORESIS PS 600V, 500MA, 150W 120VAC

EV2650-US ea
EUR 750

ELECTROPHORESIS PS 300V, 1000MA, 150W 120 VAC

EV2310-US ea
EUR 750

ELECTROPHORESIS PS 300V, 2000MA, 300W 120 VAC

EV3020-US ea
EUR 1060

ELECTROPHORESIS PS 600V, 1000MA, 300W 120 VAC

EV3610-US ea
EUR 1060

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 410

SDS-PAGE Electrophoresis Buffer

MBS355536-1L 1L
EUR 165

SDS-PAGE Electrophoresis Buffer

MBS355536-5x1L 5x1L
EUR 480

SDS-PAGE Electrophoresis Buffer

MBS355542-10x1L 10x1L
EUR 250

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Starch (Smithies); for electrophoresis

GE6857 100g
EUR 253.58

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

Aminoacetic acid, ?For electrophoresis

GRM2233-100G 1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis

Aminoacetic acid, ?For electrophoresis

GRM2233-500G 1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

Agarose, medium EEO ?For electrophoresis

RM6249-100G 1 unit
EUR 119.06
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-10G 1 unit
EUR 19.63
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-1KG 1 unit
EUR 1089.43
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-25G 1 unit
EUR 36.87
Description: Agarose, medium EEO ?For electrophoresis

Acrylamide, suitable for electrophoresis

GK5651 100g
EUR 21.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Alkaline Hemoglobin Electrophoresis Kit

MBP001-112RUNS 1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP001-224RUNS 1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP008-224RUNS 1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Paper Electrophoresis Kit (Cell

MBP009-20RUNS 1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell

Electrophoresis Power Supply (2 termina

LA842-1NO 1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina

Agarose Gel Electrophoresis Kit (Teaching)

66602 15 expt. Kit
EUR 44.05
Description: Part E

WSHT-Basic Electrophoresis Power Supply

HTES1001 set
EUR 557.2

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 90

20X DoubleQuick DNA Electrophoresis Buffer

73530 250 ml
EUR 35.58
Description: Part E

20X DoubleQuick DNA Electrophoresis Buffer

73530-1 500 ml
EUR 64.4
Description: Part E

Bovine Serum Albumin, 98% by electrophoresis

GE3699 10g
EUR 463.47

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

HiPer® Agarose Gel Electrophoresis Teach

HTBM001-10PR 1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 70

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 100

WSHT-Universal Electrophoresis Power Supply

HTES1003 set
EUR 784

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 70

Ammonium Persulphate (APS) for electrophoresis, 99%

28575 25 Gms
EUR 1.51
Description: Part A

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Electrophoresis baffle for Life Technology Novex

HTDB02 pcs
EUR 2.8

Electrophoresis Module for Eco-Maxi, for 2 gels

AJ846-017-475 each
EUR 997.35

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 91.56

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 173.27

WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell

HTES1013 set
EUR 602

WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell

HTES1014 set
EUR 448

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 450

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 470

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 410

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 610

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 710

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 750

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 21.21

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 28.49

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 43.44

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 77.25

WSHT-Multi-Functional Electrophoresis Power Supply

HTES1004 set
EUR 896

WSHT-liteDNA Universal Horizontal Electrophoresis Cell

HTES1007 set
EUR 259

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

WSHT-midiDNA Multipurpose Horizontal Electrophoresis Cell

HTES1006 set
EUR 278.6

High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa)

MKPZ010-200mL 200μL
EUR 72

SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)

E37F007-100-uL 100 μL
EUR 227
Description: Reagents for ssDNA or RNA electrophoresis

SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)

E37F007-50-uL 50 μL
EUR 133
Description: Reagents for ssDNA or RNA electrophoresis

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-005mL 0.05mL
EUR 195

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-01mL 0.1mL
EUR 255

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-5x005mL 5x0.05mL
EUR 820

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-5x01mL 5x0.1mL
EUR 1100

SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)

E37F001-100-uL 100 μL
EUR 227
Description: Reagents for dsDNA electrophoresis

SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)

E37F001-500-uL 500 μL
EUR 983
Description: Reagents for dsDNA electrophoresis

SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)

E37F001-50uL 50 μL
EUR 133
Description: Reagents for dsDNA electrophoresis

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-005mL 0.05mL
EUR 180

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-01mL 0.1mL
EUR 230

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-05mL 0.5mL
EUR 695

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-5x005mL 5x0.05mL
EUR 760

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-5x01mL 5x0.1mL
EUR 980

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

ElectrophoresisPowerSupply

SPW-6S set
EUR 349

ElectrophoresisPowerSupply(DualModule,Programmable)

SPW-DSE set
EUR 499

Electro blot Mini system 10x10cm - EACH

EBM10 EACH
EUR 1092.15

Complete OmniPAGE Mini system with electroblotting module - EACH

CVS10CBS EACH
EUR 1044.32

Electrophoretic Tissue Clearing Solution (12x1L)

LBC13001 each
EUR 741.2

VCV VERTICAL ELECTROPHORSIS

IB62000 SYSTEM
EUR 691.24

Buffer tank E for Eco-Mini (no lid, for electropho

AJ846-017-169 each
EUR 322.64

Recombinant Electrophorus electricus Hemoglobin subunit alpha (hba)

MBS1133026-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1065

Recombinant Electrophorus electricus Hemoglobin subunit alpha (hba)

MBS1133026-002mgEColi 0.02mg(E-Coli)
EUR 650

Recombinant Electrophorus electricus Hemoglobin subunit alpha (hba)

MBS1133026-002mgYeast 0.02mg(Yeast)
EUR 815

Recombinant Electrophorus electricus Hemoglobin subunit alpha (hba)

MBS1133026-01mgEColi 0.1mg(E-Coli)
EUR 755

Recombinant Electrophorus electricus Hemoglobin subunit alpha (hba)

MBS1133026-01mgYeast 0.1mg(Yeast)
EUR 955

Recombinant Electrophorus electricus Hemoglobin subunit beta (hbb)

MBS1121575-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1070

Recombinant Electrophorus electricus Hemoglobin subunit beta (hbb)

MBS1121575-002mgEColi 0.02mg(E-Coli)
EUR 655

Recombinant Electrophorus electricus Hemoglobin subunit beta (hbb)

MBS1121575-002mgYeast 0.02mg(Yeast)
EUR 820

Recombinant Electrophorus electricus Hemoglobin subunit beta (hbb)

MBS1121575-01mgEColi 0.1mg(E-Coli)
EUR 760

Recombinant Electrophorus electricus Hemoglobin subunit beta (hbb)

MBS1121575-01mgYeast 0.1mg(Yeast)
EUR 960

Recombinant Electrophorus electricus Sodium channel protein, partial

MBS1228867-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1130

Recombinant Electrophorus electricus Sodium channel protein, partial

MBS1228867-002mgEColi 0.02mg(E-Coli)
EUR 760

Recombinant Electrophorus electricus Sodium channel protein, partial

MBS1228867-002mgYeast 0.02mg(Yeast)
EUR 920

Recombinant Electrophorus electricus Sodium channel protein, partial

MBS1228867-01mgEColi 0.1mg(E-Coli)
EUR 915

Recombinant Electrophorus electricus Sodium channel protein, partial

MBS1228867-01mgYeast 0.1mg(Yeast)
EUR 1075

Recombinant Electrophorus electricus Glutamate dehydrogenase

MBS1255074-002mgBaculovirus 0.02mg(Baculovirus)
EUR 975

Recombinant Electrophorus electricus Glutamate dehydrogenase

MBS1255074-002mgEColi 0.02mg(E-Coli)
EUR 540

Recombinant Electrophorus electricus Glutamate dehydrogenase

MBS1255074-002mgYeast 0.02mg(Yeast)
EUR 730

Recombinant Electrophorus electricus Glutamate dehydrogenase

MBS1255074-01mgEColi 0.1mg(E-Coli)
EUR 635

Recombinant Electrophorus electricus Glutamate dehydrogenase

MBS1255074-01mgYeast 0.1mg(Yeast)
EUR 855

Recombinant Electrophorus electricus Acetylcholinesterase (ache), partial

MBS1164504-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1110

Recombinant Electrophorus electricus Acetylcholinesterase (ache), partial

MBS1164504-005mgEColi 0.05mg(E-Coli)
EUR 795

In addition, the Trizol method with a commercial cleaning kit produces the best 2-daut profiles in terms of background clarity, the number of spots and resolution of the Proteins-based approach. Trizol offers better choices than traditional protein extraction methods for 2-de analysis. Medaka Marines. The modified version of the Trizol method with a commercial cleaning kit is proven to produce the best 2-de profile.

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