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Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Posted by Troy

Various genetic-based approaches including mutant population screens, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. However, this genetic tool is not without a limitation attached. One alternative approach to studying meiosis plants, especially in polypoid such as Triticum Aestivum L. (wheat bread), is proteomic.

However, protein-based approaches using proteomics are rarely explained, with only two efforts to study the initial plant meiosis reported. Here, we report an inquiry of Meiosis wheat early bread using proteomics. Five protein spots that differed differently were identified using 2D gel electrophoresis (2DGE) in protein extract from four stages of Pound Meiosis and three genotypes (Chinese spring wild type, PH1B and PH2A wheat mutants). Tandem Mass Spectrometry (MS / MS / MS) Identification of peptides from these protein spots causes insulation and characterization of full length clones of wheat speckle type Puze proteins, proteins such as SF21 and HSP70, and the order of partial coding.

Hexose transporter. Significantly, the pale function of the protein POZ type Speckle and HSP70 is confirmed using the DNA binder test in vitro. Through the use of a 2DGE proteomic approach, we show that proteomic is a feasible alternative for genetic-based approaches when studying meiosis in wheat. More significant, we report a potential role for speckle type PUZ proteins and HSP70 in chromosome pairs during the early stages of meiosis on wheat bread.

Assessment of genotoxic effects of pendiumphalin in Chinese hamsters over cells with a single cell electrophoresis test (comet)

PendiMethalin (N-ethylpropopyl) -3.4-dimethyl-2,6-dinitrobenzenine) is a dinitroaniline herbicide compound that selectively controls weeds. This is cell division and growth inhibitors. It goes down the plant in a short time after Seedling. This is a soil and water pollutan because of the widespread use of formulations in Turkey and throughout the world. PendiMethalin is produced and imported by Turkey. PendiMethalin is a slightly toxic compound that is classified in class 3 toxicity by the United States Environmental Protection Agency (USPA). Although classified as Group C (human maybe carcinogens) compound by USEPA, there are a number of studies of their genotoxic effects. The purpose of this study was to evaluate vitro genotoxic effects from various methalized concentrations in the top Chinese hamsters (CHO) cells with a single cell gel electrophoresis test (comet).

Materials and Methods: Cells incubated with a concentration of pendinalin 1, 10, 100, 100, 1000 and 10000 μm for 30 minutes at 37 ° C and DNA damage compared to Cho cells that are not treated with pendiMethaline. 50 μm hydrogen peroxide is used as a positive control.

Results: There are no significant cytotoxic effects observed in the concentration range studied. DNA damage in Cho cells increased significantly in the concentration of pendiDalinalin 1, 100, 1000 and 10000 μm, a significant decrease was observed in the concentration of 10 μm pendiumphalization.

The application of capillary zone electrophoresis (CZE) and capillary gel electrophoresis (CGE) for the separation of human insulin, lispro insulin and their degradation products.

The method of two capillar electrophoresis (CE) has been developed for the separation of cars and mass variants of human insulin and recombinous analogues of Lispro. Because the capillar zone (CZE) and capillar gel electrophoresis (CGE) are based on different separation principles, they can be used to detect different insulin impurities and analogues. The application of CZE allows the separation of compounds with different M / Z ratios, therefore CZE is a suitable method for the separation of Deamidation Insulin products. After optimization, this method is validated according to the requirements of ICH. The CGE method is used for separation of higher molecular weight transformation products. Experimental data shows that CZE and CGE are simple, fast and strong methods that can be used as routine analysis for the quality control of insulin formulation

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics.

Production of high-quality two-dimensional gel electrophoresis profiles for MEDAKA Marine samples using trizol-based protein extraction approaches.

Medaka Marines is one of the most popular species of fish species for ecotoxicology and environmental research and proteomic studies is a tool that is useful for understanding Medaka molecular responses on exposure with environmental stressors. The preparation of high-quality protein samples is the key to producing high-quality two-dimensional electrophoresis gel results (2-DE) for proteomic analysis. In recent years, trizol-based protein extraction has gained popularity because of its promising performance in producing high-quality 2-de and convenience methods.

Three Trizol-Based Approaches (Trizol Methods, Trizol Alikuot Methods and Trizol Methods with Commercial Cleaning Kits) are used to extract proteins from Medaka Marine samples and 2-de profiles produced. 2-de profile quality and effectiveness of extraction methods evaluated. In comparison, two common protein extraction methods (methods of buffer lysis and extraction of trichodic acid / acetone precipitation) are also applied in parallel with a trizol-based approach. Each trizol-based approach produces high-quality 2-de high-quality Marine Profile Medaka compared to the two methods of Buffer Lisis and TCA / Aceton rainfall extraction.

mini system 7x8 cm

EHS3100-SYS ea
EUR 437

mini vertical system 10x10 cm

EVS1100-SYS ea
EUR 606

mini vertical system 20x10 cm

EVS1200-SYS ea
EUR 859

CYFRA211 (Keratin 21-1) ELISA test

23 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CYFRA211 (Keratin 21-1)

electrophoresis power supply

EV245 ea
EUR 479

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit

MEA384Mu-10x96wellstestplate 10x96-wells test plate
EUR 3645.03
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit

MEA384Mu-1x48wellstestplate 1x48-wells test plate
EUR 387.83
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit

MEA384Mu-1x96wellstestplate 1x96-wells test plate
EUR 511.18
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit

MEA384Mu-5x96wellstestplate 5x96-wells test plate
EUR 2000.31
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 23 (IL23) ELISA Kit

4-MEA384Mu
  • EUR 3696.00
  • EUR 1951.00
  • EUR 512.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Interleukin 23 (IL23) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit

MEA384Ra-10x96wellstestplate 10x96-wells test plate
EUR 5523.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit

MEA384Ra-1x48wellstestplate 1x48-wells test plate
EUR 542.52
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit

MEA384Ra-1x96wellstestplate 1x96-wells test plate
EUR 732.17
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit

MEA384Ra-5x96wellstestplate 5x96-wells test plate
EUR 2994.77
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Rat Interleukin 23 (IL23) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit

4-MEA384Ra
  • EUR 5574.00
  • EUR 2945.00
  • EUR 733.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Rat Interleukin 23 (IL23) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Mini Samples Interleukin 23 ELISA Kit (IL23)

RK02941 96 Tests
EUR 521

Common diseases of the cardiovascular system, 23 cases (2mm)

CVD231 1
EUR 190
Description: Cardiovascular disease tissue array, non-overlapping with CVD481, 23 cases of normal, reactive and neoplastic conditions of the heart and blood vessels. Designed for blood vessel or endothelial cell related studies.

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 625.5

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 309

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 181

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 54

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 482

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 342

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3124.4

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit

MEA746Mu-10x96wellstestplate 10x96-wells test plate
EUR 5242.01
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit

MEA746Mu-1x48wellstestplate 1x48-wells test plate
EUR 519.34
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit

MEA746Mu-1x96wellstestplate 1x96-wells test plate
EUR 699.06
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit

MEA746Mu-5x96wellstestplate 5x96-wells test plate
EUR 2845.77
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23).

Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) ELISA Kit

4-MEA746Mu
  • EUR 5293.00
  • EUR 2796.00
  • EUR 700.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Fibroblast Growth Factor 23 (FGF23) in samples from n/a with no significant corss-reactivity with analogues from other species.

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7
EUR 744

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 479

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 749

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 572

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 749

electrophoresis power supply uk plug

EV245-UK ea
EUR 479

electrophoresis power supply 120 vac

EV245-US ea
EUR 479

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 572

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 572

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 572

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 800

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1214

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 800

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1214

Anti-Dimethyl Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone#RM181

M06819-23 100ug
EUR 468
Description: Anti-Dimethyl Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone#RM181 tested in WB, ELISA, Multiplex, ChIP, IHC, reactive to All Vertebrates

IL-12, Interleukin-12, human

RC212-23 2ug
EUR 104.38

Endostatin, human

RC214-23 20ug
EUR 104.38

Betacellulin, human

RC218-23 5ug
EUR 104.38

S100B, monkey

RC230-23 2ug
EUR 104.38

Betacellulin, murine (mouse)

RC238-23 5ug
EUR 104.38

CCL12, murine (mouse)

RC335-23 5ug
EUR 101.33

UBC12, Ubiquitin Conjugating Enzyme 12 (His6-tagged), human

RC612-23 50ug
EUR 169.63

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 319.26

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 406.26

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 406.26

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 428

ProtoBlock System

NAT1004 1KIT
EUR 102

Interleukin-23/IL-23

E21-S31 10ug
EUR 343

mini-ANP

5-01547 4 x 1mg Ask for price

Mini Centrifuge

CTFG 1 unit
EUR 89
Description: Small Centrifuge used for preparation of fish and wine samples for use with the QuantiQuikâ„¢ Histamine Quick Test Strips .

Mini BlotBoy

B3D1008-E 1 PC
EUR 437.43

Roto-Mini

R2020-E 1 PC
EUR 391.18

Roto-Mini

R2024-E 1 PC
EUR 499.13

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 165

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 151

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 570.8

Claudin 23 (Claudin 23) Antibody

abx231737-100ug 100 ug
EUR 481

data acquisition system

D230 ea
EUR 1704

photo documentation system

VLBPCX4-20M ea
EUR 6352

photo documentation system

VLBPCX4-20MX ea
EUR 6774

photo documentation system

VLDP-HOOD ea
EUR 5084

photo documentation system

VLDP17-26M ea
EUR 8126

photo documentation system

VLDP17-26MX ea
EUR 8464

Fast Mutagenesis System

20-abx098073
  • EUR 509.00
  • EUR 592.00
  • 10 rxns
  • 20 rxns

CCD cooling system

3600100 1unit
EUR 4914

Cometslides Rack System

4252-040-02 1 Each
EUR 213.8

In addition, the Trizol method with a commercial cleaning kit produces the best 2-daut profiles in terms of background clarity, the number of spots and resolution of the Proteins-based approach. Trizol offers better choices than traditional protein extraction methods for 2-de analysis. Medaka Marines. The modified version of the Trizol method with a commercial cleaning kit is proven to produce the best 2-de profile.

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