Medaka Sea is one of the most popular models of fish species for Ecotoxicology and environmental research and study of proteomics is a useful tool for understanding the medaka molecular response after exposure to different environmental stresses. High-quality protein sample preparation is the key to producing high-quality two-dimensional gel electrophoresis (2-DE) results for proteomics analysis.

In recent years, the extraction of protein-based Trizol has gained popularity because of its performance promise in producing high-quality 2-DE and comfort method.Three Trizol-based approach (Trizol method, the method and the method of Trizol Trizol aliquots with commercial cleaning kit) is used to extract protein from marine samples medaka and 2-DE profiles produced. 2-DE profile quality and effectiveness of extraction methods were evaluated.

For comparison, two common protein extraction method (method of lysis buffer and trichloroacetic acid (TCA) / acetone precipitation extraction) are also applied in parallel to Trizol based approaches.Any three Trizol-based approach produces high-quality 2-DE profiles of marine medaka compared with both methods of lysis buffer and TCA / acetone precipitation extraction.

In addition, the Trizol method with clean-up kit produced the best commercial 2-DE profile in terms of the clarity of the background, the number of spots and resolution proteins.Trizol based approach offered a better choice than traditional protein extraction method for 2-DE analysis of medaka sea. A modified version of the method Trizol with commercial clean-up kit was shown to produce the best 2-DE profile.

A method of combining blue native polyacrylamide gel electrophoresis liquid chromatography-tandem mass spectrometry for the detection of circulating immune complexes between the monoclonal antibody therapy and anti-drug antibodies in animals.

Monoclonal antibody therapy can potentially induce unwanted immune response, so that the production of anti-drug antibodies (ADAS). Binding of fennel drug and subsequent formation of immune complexes (ICs) can trigger a variety of responses, depending on the size, concentration, and a subclass of fennel. To better understand the impact of fennel in pharmacokinetics, pharmacodynamics and toxicology profile, bioanalytical methods developed to detect IC between a human monoclonal immunoglobulin G (IgG) and fennel in a biological sample.

Regarding the experimental procedure, in brief, human-specific antibodies and human antibodies bound IC in biological samples separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The target fraction is then cut from the gel, followed by in-gel trypsin digestion and subsequent liquid chromatography-tandem mass spectrometry (LC-MS / MS) to monitor the human peptide-specific IgG. This method is capable of detecting various types of human antibodies with a lower detection limit of 10 ug / mL in monkey serum. Performance IC assay to detect the expressed using spiked samples, and pre-incubated in monkey serum IC clearly detectable.

5 gel caster for 8 x 10cm or 10 x 10cm gels.
CSL6CAST	Scientific Laboratory Supplies	EACH	EUR 508.44

ES Horizontal Electrophoresis Set
LE1011	GenDepot	Set	EUR 578.4

Dummy Plate 10 x 10cm
VS10DP	Scientific Laboratory Supplies	EACH	EUR 23.94

20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System
LE1000C10-10	GenDepot	Ea	EUR 153.6

10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System
LE1000C10-01	GenDepot	Ea	EUR 153.6

10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System
LE1000C10-02	GenDepot	Ea	EUR 153.6

AXYGEN® HORIZONTAL GEL BOX, 10 CM
HGB-10	CORNING	1/pk	EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

Casting Dams, For Enduro 10cm Gel System
LE1000CD-02	GenDepot	Ea	EUR 165.6

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs
ELE2302	Scientific Laboratory Supplies	EACH	EUR 1520.76

Multi Sub Midi 10 x 10cm UV Tray
MS10UV10	Scientific Laboratory Supplies	EACH	EUR 79.8

Vert Sub Mini 10 x 10cm Dual Caster
VS10DCAST	Scientific Laboratory Supplies	EACH	EUR 196.08

Replacement Rubber mats for 10 x 10cm caster
VS10DCASTM	Scientific Laboratory Supplies	EACH	EUR 50.16

Burnsoothe Burn Relief Dressing 10cm x 10cm
SAF1294	Scientific Laboratory Supplies	PK10	EUR 47.88

CoolTherm burn relief dressing 10cm x 10cm
SAF1330	Scientific Laboratory Supplies	PK10	EUR 164.16

99445-10 DCT 10 X 75MM
99445-10	CORNING	250/pk	EUR 75.6
Description: Disposable Culture Tubes; DCT's, CGW

6 x 15ml horizontal rotisserie
R4040-HZ15	Benchmark Scientific	1 PC	EUR 127.76

6 x 50ml horizontal rotisserie
R4040-HZ50	Benchmark Scientific	1 PC	EUR 197.63

5 x SDS Electrophoresis Buffer
GR103039	Genorise Scientific	1 L	EUR 128.4

C18 3um 10cm x 3mm
CHR4804	Scientific Laboratory Supplies	EACH	EUR 644.1

AQ 3um 10cm x 2.1mm
CHR4990	Scientific Laboratory Supplies	EACH	EUR 664.62

AQ 5um 10cm x 4.6mm
CHR4992	Scientific Laboratory Supplies	EACH	EUR 607.62

GC18 4um 10cm x 4.6mm
CHR5004	Scientific Laboratory Supplies	EACH	EUR 579.12

Dummy Plate 20 x 10cm
VS10WDP	Scientific Laboratory Supplies	EACH	EUR 26.22

12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System
LE1000C10-03	GenDepot	Ea	EUR 153.6

12 Well Comb ,1mm Thick For Enduro 10cm Gel System
LE1000C10-04	GenDepot	Ea	EUR 153.6

12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System
LE1000C10-05	GenDepot	Ea	EUR 153.6

16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System
LE1000C10-06	GenDepot	Ea	EUR 153.6

16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System
LE1000C10-07	GenDepot	Ea	EUR 153.6

16 Well Comb ,2mm Thick For Enduro 10cm Gel System
LE1000C10-08	GenDepot	Ea	EUR 153.6

25 Well Comb ,1mm Thick For Enduro 10cm Gel System
LE1000C10-11	GenDepot	Ea	EUR 153.6

8 Well Comb ,1mm Thick For Enduro 10cm Gel System
LE1000C10-12	GenDepot	Ea	EUR 153.6

10 x 10cm Notched Glass Plates 2mm thick Pack of 2
VS10NG	Scientific Laboratory Supplies	PK2	EUR 38.76

Horizontal head, for 12 x 1.5ml
BCM1604	Bio Basic	1 pcs, 1 UNIT	EUR 200.68

Horizontal head, for 4 x 15ml
BCM1605	Bio Basic	1 pcs, 1 UNIT	EUR 200.68

Horizontal head, for 2 x 50ml
BCM1606	Bio Basic	1 pcs, 1 UNIT	EUR 200.68

Horizontal head for 12 x 1.5mL
MIX7018	Scientific Laboratory Supplies	EACH	EUR 121.98

Horizontal head for 4 x 15mL
MIX7020	Scientific Laboratory Supplies	EACH	EUR 121.98

Horizontal head for 2 x 50mL
MIX7022	Scientific Laboratory Supplies	EACH	EUR 121.98

4 x Phosphate/SDS Electrophoresis Buffer
GR103033	Genorise Scientific	1 L	EUR 139.2

SU C18 3.5u 10cm x 3.0mm
CHR4376	Scientific Laboratory Supplies	EACH	EUR 687.35

C18 5um 10cm x 30mm id
CHR4826	Scientific Laboratory Supplies	EACH	EUR 4822.2

C18 Polar 3u 10cm x 2.1mm
CHR4864	Scientific Laboratory Supplies	EACH	EUR 643.61

MSP-C4 5um 10cm x 2.1mm
CHR5014	Scientific Laboratory Supplies	EACH	EUR 593.62

MSP-C4 5um 10cm x 3.0mm
CHR5016	Scientific Laboratory Supplies	EACH	EUR 593.62

MSP-C4 5um 10cm x 4.0mm
CHR5018	Scientific Laboratory Supplies	EACH	EUR 593.62

MDC-C18 5um 10cm x 3.0mm
CHR5048	Scientific Laboratory Supplies	EACH	EUR 612.36

MDC-C18 5um 10cm x 4.0mm
CHR5050	Scientific Laboratory Supplies	EACH	EUR 612.36

MDC-C18 5um 10cm x 4.6mm
CHR5052	Scientific Laboratory Supplies	EACH	EUR 612.36

Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611	Bio Basic	1 UNIT	EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613	Bio Basic	1 UNIT	EUR 487.51

10-well protein Vertical Electrophoresis Apparatus
EDK692	Bio Basic	1 UNIT	EUR 487.51

20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System
LE1000C10-09	GenDepot	Ea	EUR 153.6

Kinesis Tubing PEEKsil 1/32 x 25um x 10cm
CHR2453	Scientific Laboratory Supplies	PK2	EUR 43.32

Kinesis TuBING PEEKsil 1/32 x 50um x 10cm
CHR2463	Scientific Laboratory Supplies	PK2	EUR 43.32

Kinesis Tubing PEEKsil 1/16 x 25um x 10cm
CHR2593	Scientific Laboratory Supplies	PK5	EUR 110.58

Kinesis Tubing PEEKsil 1/16 x 50um x 10cm
CHR2611	Scientific Laboratory Supplies	PK5	EUR 102.6

Kinesis Tub SS 1/32 x 205um x 10cm
CHR4823	Scientific Laboratory Supplies	EACH	EUR 13.68

Kinesis Tub SS 1/32 x .015 x 10cm
CHR4841	Scientific Laboratory Supplies	EACH	EUR 20.52

6 x 15mL horizontal rotisserie 1 ea
MIX7080	Scientific Laboratory Supplies	EACH	EUR 76.38

6 x 50mL horizontal rotisserie 1 ea
MIX7082	Scientific Laboratory Supplies	EACH	EUR 76.38

ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1	GenDepot	Ea	EUR 198

ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2	GenDepot	Ea	EUR 181.2

CoolTherm burn relief dressing 10cm x 40cm
SAF1332	Scientific Laboratory Supplies	PK7	EUR 152.76

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs
ELE2300	Scientific Laboratory Supplies	EACH	EUR 1448.94

midiplus horizontal unit 15x7-10-15 cm
EHS1300-SYS	Consort	ea	EUR 496.8

Genorise® 10 x RNA Sample Loading Buffer
GR102010-10	Genorise Scientific	10 mL	EUR 177.6

Nitrocellulose Membrane, 0.25 µm, 9 cm x 10cm
AR0135-02	BosterBio	20 sheets/Pack	EUR 115.2

Nitrocellulose Membrane, 0.45 µm, 9 cm x 10cm
AR0135-04	BosterBio	20 sheets/Pack	EUR 115.2

Multi Sub Choice 15 x 10cm UV Tray
MS15UV10	Scientific Laboratory Supplies	EACH	EUR 93.48

Multi Sub Maxi 20 x 10cm UV Tray
MS20UV10	Scientific Laboratory Supplies	EACH	EUR 124.26

Multi Sub Mini 7 x 10cm UV Tray
MS7UV10	Scientific Laboratory Supplies	EACH	EUR 72.96

LU C18 (2) 3um 10cm x 3mm id
CHR3964	Scientific Laboratory Supplies	EACH	EUR 643.61

Horizontal head
BV1000-H15	Benchmark Scientific	1 PC	EUR 161.26

Horizontal head
BV1000-H150	Benchmark Scientific	1 PC	EUR 161.26

Horizontal head
BV1000-H500	Benchmark Scientific	1 PC	EUR 2813.28

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)
EDK614	Bio Basic	1 UNIT	EUR 520.92

Daunomycin (10.10), DNA Aptamer, Biotinylated
AD-113-B	Alpha Diagnostics	Custom	Ask for price

Daunomycin (10.10), DNA Aptamer, unlabeled
AD-113-U	Alpha Diagnostics	Custom	Ask for price

Technical Buffer pH 10.10 250ml
51350010	Scientific Laboratory Supplies	EACH	EUR 25.99

MAGic Clamp Tube Rack 4 x 15mL tubes horizontal
MIX7174	Scientific Laboratory Supplies	EACH	EUR 133.38

MAGic Clamp Tube Rack 2 x 50mL tubes horizontal
MIX7176	Scientific Laboratory Supplies	EACH	EUR 133.38

Kinesis Tubing SS 1/16od x 0.02id - 10cm Length
CHR4789	Scientific Laboratory Supplies	EACH	EUR 5.7

Kinesis Tubing SS 1/32 x 0.018 - 10cm Length
CHR4849	Scientific Laboratory Supplies	EACH	EUR 20.52

Kinesis Tubing SS 1/16od x 0.03id - 10cm Length
CHR4855	Scientific Laboratory Supplies	EACH	EUR 7.98

Kinesis Tubing SS 1/16 x .040 - 10cm Length
CHR4887	Scientific Laboratory Supplies	EACH	EUR 10.26

Kinesis Tubing SS 1/16 x .046 - 10cm Length
CHR4901	Scientific Laboratory Supplies	EACH	EUR 11.4

AXYGEN® GEL TRAY 10 X 10 CM FOR USE WITH 10 CM GEL BOX, UV TRANSPARENT
HGB10-10-GT	CORNING	1/pk	EUR 88.8
Description: Lab Equipment; Axygen Branded EQ

ES 8/10 Double Gel Comb For ES Electrophoresis
LE1011-A3	GenDepot	Ea	EUR 148.8

GELS Polyclonal Antibody
ES11007-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against GELS from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

GELS Polyclonal Antibody
ES11007-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against GELS from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

GELS Polyclonal Antibody
ABP58629-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of GELS from Human, Mouse, Rat. This GELS antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GELS protein

GELS Polyclonal Antibody
ABP58629-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of GELS from Human, Mouse, Rat. This GELS antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GELS protein

GELS Polyclonal Antibody
ABP58629-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of GELS from Human, Mouse, Rat. This GELS antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GELS protein

Anti-GELS antibody
STJ192165	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to GELS

20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System
LE1000C15-10	GenDepot	Ea	EUR 157.2

50 Well Comb ,2mm Thick For Enduro 20cm Gel System
LE1000C20-10	GenDepot	Ea	EUR 165.6

Gel Tray 10 X 10 Cm
LE1000GT-03	GenDepot	Ea	EUR 170.4

ENDURO™ PowerStack
LE0304S	GenDepot	Ea	EUR 724.8

Horizontal MicroLoops E
M8-L18SP-15H	MiTeGen	20 MOUNTS	EUR 112
Description: Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

Horizontal MicroLoops E
M8-L18SP-50H	MiTeGen	20 MOUNTS	EUR 112
Description: Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

Daunomycin (10.10), DNA Aptamer, FITC labeled
AD-113-F	Alpha Diagnostics	Custom	Ask for price

electrophoresis power supply
EV245	Consort	ea	EUR 574.8

CometAssay Electrophoresis System
4250-050-ES	Biotechne	1 Kit	EUR 3540.96

miniONE Electrophoresis System
ELE3400	Scientific Laboratory Supplies	EACH	EUR 624.72

MAGic Clamp Tube Rack 12 x 1.5/2.0mL tubes horizontal
MIX7172	Scientific Laboratory Supplies	EACH	EUR 133.38

TBE Bufer, 10 x
GR103009	Genorise Scientific	1 L	EUR 99.6

TBST Bufer, 10 x
GR103010	Genorise Scientific	1 L	EUR 128.4

TE Bufer, 10 x
GR103012	Genorise Scientific	500 mL	EUR 190.8

Silicon Septa x 10
WAT4826	Scientific Laboratory Supplies	PK10	EUR 144.78

MultiBox for Western blotting, 4 compartments, 38 x 71 x 28mm each (11/2 x 2 13/16 x 11/8 in.), for various gels including half-mini protein gels
B1204	MTC Bio	6/pack	EUR 119.14
Description: Western Blot Boxes

Kinesis Tubing SS 1/32 x 250um (0.010) - 10cm Length
CHR4831	Scientific Laboratory Supplies	EACH	EUR 21.66

Kinesis Tubing SS 1/16 x 180um(.007in) - 10cm Length
CHR4863	Scientific Laboratory Supplies	EACH	EUR 6.84

Kinesis Tubing SS 1/16 x 125um(.005in) - 10cm Length
CHR4915	Scientific Laboratory Supplies	EACH	EUR 6.84

9 x 10cm Notched Glass Plates with 0.75mm Bonded Spacers
VS10NGS075	Scientific Laboratory Supplies	EACH	EUR 53.58

9 x 10cm Plain Glass Plates with 0.75mm Bonded Spacers
VS10PGS075	Scientific Laboratory Supplies	EACH	EUR 35.34

19 x 10cm Notched Glass Plates with 0.75mm Bonded Spacers
VS10WNGS075	Scientific Laboratory Supplies	EACH	EUR 125.4

19 x 10cm Notched Glass Plates with 1mm Bonded Spacers
VS10WNGS1	Scientific Laboratory Supplies	EACH	EUR 132.24

Accuris Gel Casting Set for 5x6cm Gels (18/10 Teeth)
ELE1114	Scientific Laboratory Supplies	EACH	EUR 86.64

10 Well Comb ,1mm Thick For Enduro 15cm Gel System
LE1000C15-01	GenDepot	Ea	EUR 157.2

10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System
LE1000C15-02	GenDepot	Ea	EUR 157.2

10 Well Comb , 1mm Thick For Enduro 7cm Gel System
LE1000C7-01	GenDepot	Ea	EUR 148.8

10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System
LE1000C7-02	GenDepot	Ea	EUR 148.8

Horizontal outer dims 415h x 140w x 560d with 11 apertures of approx 37mm
AH17911	Scientific Laboratory Supplies	EACH	EUR 149.34

Horizontal outer dims 415h x 140w x 560d with 6 apertures of approx 67mm
AH1796UT	Scientific Laboratory Supplies	EACH	EUR 168.72

X-NeuNAc
A3944-10	ApexBio	10 mg	EUR 157.2
Description: Km: 0.89 mM for neuraminidase X-Neu5Ac is a new substrate for chromogenic assay of neuraminidase activity in bacterial expression systems.

Tenacissoside X
N2110-10	ApexBio	10 mg	EUR 686.4
Description: Extracted from Marsdenia tenacissima (Roxb.) Wight et Arn.;Store the product in sealed, cool and dry condition

Platform for 10 x 10 mL tubes
MIX1256	Scientific Laboratory Supplies	EACH	EUR 47.88

Digital Hotplate, 10 x 10 inch, 115V
H3710-H	Benchmark Scientific	1 each	EUR 545.3

Digital Hotplate, 10 x 10 inch, 230V
H3710-H-E	Benchmark Scientific	1 each	EUR 545.3

Magnetic Stirrer, 10 x 10 inch, 115V
H3710-S	Benchmark Scientific	1 each	EUR 545.3

Magnetic Stirrer, 10 x 10 inch, 230V
H3710-SE	Benchmark Scientific	1 each	EUR 545.3

DIGE gel 3 gels
28937451	Scientific Laboratory Supplies	EACH	EUR 486.78

1730 10 SNAP-SEAL 10 OZ
1730-10	CORNING	100/pk	EUR 96
Description: Disposable Plastic; Plastic Containers

9 x 10cm Plain Glass Plates 2mm thick Pack of 2
VS10PG	Scientific Laboratory Supplies	EACH	EUR 28.5

GSA 10
B5767-10	ApexBio	10 mg	EUR 274.8

SC-10
B6299-10	ApexBio	10 mg	EUR 255.6

MRT 10
B7685-10	ApexBio	10 mg	EUR 285.6

Kodak X-Ray Film 8 x 10 inch
B503	ABM	100 Sheets	EUR 242.4

Horizontal 50mL Tube Holder
MIX5088	Scientific Laboratory Supplies	EACH	EUR 144.78

Pipette Tray PVC Horizontal
PIP5500	Scientific Laboratory Supplies	EACH	EUR 38.76

Midi Horizontal Gel Unit
ELE8120	Scientific Laboratory Supplies	EACH	EUR 630.42

Horizontal orientator 384 wells
DD53423	Scientific Laboratory Supplies	PK2	EUR 63.84

10 x Cell Lysis Buffer
GR103003x10	Genorise Scientific	200 mL	EUR 141.6

10 x PBS, pH 7.30
GR103004x10	Genorise Scientific	1 L	EUR 100.8

10 x Universal Blocking Buffer
GR103005	Genorise Scientific	200 mL	EUR 154.8

10 x ELISA Wash Buffer
GR103014	Genorise Scientific	1 L	EUR 117.6

10 x ELISA Reagent Diluent
GR103028	Genorise Scientific	100 mL	EUR 92.4

Hypercassette Neutral 10 X 12In
RPN11650	Scientific Laboratory Supplies	EACH	EUR 367.08

4 X 10 CARBON CARTRIDGE
CDFC410F2	Scientific Laboratory Supplies	EACH	EUR 295.26

Bellows pasteur pipette x 10
WAT4772	Scientific Laboratory Supplies	PK10	EUR 144.78

CometAssay Electrophoresis Starter Kit
4250-050-ESK	Biotechne	1 Kit	EUR 3749.28

Agarose for DNA electrophoresis
16-0027-500	Medicago	500 g	EUR 410.4

myGel Mini Electrophoresis System
BM0286	GenDepot	System	EUR 741.6

Acrylamide, suitable for electrophoresis
GK5651-100G	Glentham Life Sciences	100 g	EUR 64.8

Starch (Smithies), for electrophoresis
GE6857-1KG	Glentham Life Sciences	1 kg	EUR 370.8

Starch (Smithies), for electrophoresis
GE6857-500G	Glentham Life Sciences	500 g	EUR 217.2

6X6 well Electrophoresis Apparatus
EDK626	Bio Basic	1 UNIT	EUR 750.6

10cm UV Gel Scoop
MS10UVS	Scientific Laboratory Supplies	EACH	EUR 51.3

Chiller for Electrophoresis systems
CSLCHILLER	Scientific Laboratory Supplies	EACH	EUR 6453.54

myGel Mini Electrophoresis System
E1101	Benchmark Scientific	1 each	EUR 623.9

Kinesis Adapt - 10-32M x 10-32F - PEEK
CHR4283	Scientific Laboratory Supplies	EACH	EUR 27.36

AI-10-49
A8694-10	ApexBio	10 mg	EUR 254.4
Description: AI-10-49 is a selective inhibitor of CBF? -SMMHC and RUNX1 interaction with a FRET IC50 value of 260nM. AI-10-49 restores RUNX1 transcriptional activity, displays favorable pharmacokinetics, and delays leukemia progression in mice.

Keratin 10 antibody
10-2541	Fitzgerald	250 ug	EUR 590.4
Description: Mouse monoclonal Keratin 10 antibody

MFZ 10-7
B5595-10	ApexBio	10 mg	EUR 420

TC ASK 10
B5728-10	ApexBio	10 mg	EUR 525.6

PSB 10 hydrochloride
B6923-10	ApexBio	10 mg	EUR 439.2

10-DEBC hydrochloride
B7105-10	ApexBio	10 mg	EUR 265.2
Description: 10-DEBC hydrochloride is a selective inhibitor of Akt (or termed PKB) [1], with an IC50 value of approximate 48 ?M [2].Akt is a type of serine/threonine kinase. It phosphorylates and inactivates components in the apoptotic machinery, including Caspase 9 and BAD.

KinaSorb™ 10
KE785-10	Biotech Support Group	10 preps	EUR 553.2

Empty Tubes (10)
2MLET-10	BioAssay Systems	10	EUR 17
Description: Empty tubes used for preparation of urine samples for use with the Oxalate Quick Test Strips. Quantity: 10 empty 2mL tubes.

Aluminum Test Tube Basket 10 x 6 x 6
HS20341B	Scientific Laboratory Supplies	EACH	EUR 40.47

Finntip 10 0.2-10ul Pink 10 x 96 rack
PIP3644	Scientific Laboratory Supplies	PK960	EUR 111.72

Cytiva ALUMINA PLATES 10 X 8 CM 10 EA
GESE202N10	Scientific Laboratory Supplies	PK10	EUR 327.18

Aluminium Tray For 10 X 10 Wide Vials 28.5mm
FLY1320	Scientific Laboratory Supplies	EACH	EUR 93.48

Taken together, these findings indicate that our method allows semi-quantitative analysis to estimate the ratio of human antibodies compared with the IC included in the total antibody. This method was successfully applied to the in vivo studies using rats, and the data helps explain the unexpectedly rapid clearance of human antibodies for the formation of large IC. The combination of IC separation by BN-PAGE and detection of peptide-specific human IgG with LC-MS / MS bioanalytical approach is common, useful for detecting IC in animals.