Medaka Sea is one of the most popular models of fish species for Ecotoxicology and environmental research and study of proteomics is a useful tool for understanding the medaka molecular response after exposure to different environmental stresses. High-quality protein sample preparation is the key to producing high-quality two-dimensional gel electrophoresis (2-DE) results for proteomics analysis.
In recent years, the extraction of protein-based Trizol has gained popularity because of its performance promise in producing high-quality 2-DE and comfort method .Three Trizol-based approach (Trizol method, the method and the method of Trizol Trizol aliquots with commercial cleaning kit) is used to extract protein from marine samples medaka and 2-DE profiles produced. 2-DE profile quality and effectiveness of extraction methods were evaluated.
For comparison, two common protein extraction method (method of lysis buffer and trichloroacetic acid (TCA) / acetone precipitation extraction) are also applied in parallel to Trizol based approaches.Any three Trizol-based approach produces high-quality 2-DE profiles of marine medaka compared with both methods of lysis buffer and TCA / acetone precipitation extraction.
In addition, the Trizol method with clean-up kit produced the best commercial 2-DE profile in terms of the clarity of the background, the number of spots and resolution proteins.Trizol based approach offered a better choice than traditional protein extraction method for 2-DE analysis of medaka sea. A modified version of the method Trizol with commercial clean-up kit was shown to produce the best 2-DE profile.
Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches.
A method of combining blue native polyacrylamide gel electrophoresis liquid chromatography-tandem mass spectrometry for the detection of circulating immune complexes between the monoclonal antibody therapy and anti-drug antibodies in animals.
Monoclonal antibody therapy can potentially induce unwanted immune response, so that the production of anti-drug antibodies (ADAS). Binding of fennel drug and subsequent formation of immune complexes (ICs) can trigger a variety of responses, depending on the size, concentration, and a subclass of fennel. To better understand the impact of fennel in pharmacokinetics, pharmacodynamics and toxicology profile, bioanalytical methods developed to detect IC between a human monoclonal immunoglobulin G (IgG) and fennel in a biological sample.
Regarding the experimental procedure, in brief, human-specific antibodies and human antibodies bound IC in biological samples separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The target fraction is then cut from the gel, followed by in-gel trypsin digestion and subsequent liquid chromatography-tandem mass spectrometry (LC-MS / MS) to monitor the human peptide-specific IgG. This method is capable of detecting various types of human antibodies with a lower detection limit of 10 ug / mL in monkey serum. Performance IC assay to detect the expressed using spiked samples, and pre-incubated in monkey serum IC clearly detectable.
ES Horizontal Electrophoresis Set
LE1011
GenDepot
Set
EUR 410
Hi-Eco Mini Horizontal Electrophoresis K
LA851-1NO
EWC Diagnostics
1 unit
EUR 205.56
Description: Hi-Eco Mini Horizontal Electrophoresis K
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH
ELE2302
Scientific Laboratory Supplies
EACH
EUR 1891.35
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH
ELE2300
Scientific Laboratory Supplies
EACH
EUR 1802.25
Electrophoresis Module for Eco-Mini, for 2 gels
AJ846-017-175
Westburg
each
EUR 604.95
Electrophoresis Module for Eco-Maxi, for 2 gels
AJ846-017-475
Westburg
each
EUR 997.35
ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1
GenDepot
Ea
EUR 100
ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2
GenDepot
Ea
EUR 90
Agarose Gel Electrophoresis Kit
MB570-10PR
EWC Diagnostics
1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit
ES 8/10 Double Gel Comb For ES Electrophoresis
LE1011-A3
GenDepot
Ea
EUR 70
electrophoresis ps 1500v, 300ma, 150w
EV2230
Consort
ea
EUR 898.8
electrophoresis ps 1500v, 300ma, 150w
EV2230-UK
Consort
ea
EUR 898.8
electrophoresis ps 1500v, 300ma, 150w
EV2230-US
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320-UK
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320-US
Consort
ea
EUR 898.8
ELECTROPHORESIS PS 1200V, 500MA, 300W
EV3150
Consort
ea
EUR 1060
ELECTROPHORESIS PS 1200V, 500MA, 300W
EV3150-UK
Consort
ea
EUR 1060
ELECTROPHORESIS PS 3000V, 200MA, 300W
EV3330
Consort
ea
EUR 1600
ELECTROPHORESIS PS 3000V, 200MA, 300W
EV3330-UK
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W
EV3620
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W
EV3620-UK
Consort
ea
EUR 1600
Electrophoresis Buffer (10x)
MBS2557002-100mL
MyBiosource
100mL
EUR 85
Electrophoresis Buffer (10x)
MBS2557002-200mL
MyBiosource
200mL
EUR 90
Electrophoresis Buffer (10x)
MBS2557002-500mL
MyBiosource
500mL
EUR 95
Electrophoresis Buffer (10x)
MBS2557002-5x500mL
MyBiosource
5x500mL
EUR 380
electrophoresis ps 400v, 500ma, 50w
EV1450
Consort
ea
EUR 574.8
electrophoresis ps 400v, 500ma, 50w
EV1450-UK
Consort
ea
EUR 574.8
electrophoresis ps 400v, 500ma, 50w
EV1450-US
Consort
ea
EUR 574.8
ELECTROPHORESIS PS 300V, 1000MA, 150W
EV2310
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 1000MA, 150W
EV2310-UK
Consort
ea
EUR 750
ELECTROPHORESIS PS 600V, 500MA, 150W
EV2650
Consort
ea
EUR 750
ELECTROPHORESIS PS 600V, 500MA, 150W
EV2650-UK
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 2000MA, 300W
EV3020
Consort
ea
EUR 1060
ELECTROPHORESIS PS 300V, 2000MA, 300W
EV3020-UK
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W
EV3610
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W
EV3610-UK
Consort
ea
EUR 1060
TEMED, electrophoresis grade
RM10358-100G
EWC Diagnostics
1 unit
EUR 301.18
Description: TEMED, electrophoresis grade
Agarose Gel Electrophoresis Kit (Teaching)
66602
Sisco Laboratories
15 expt. Kit
EUR 44.05
Description: Part E
electrophoresis power supply
EV245
Consort
ea
EUR 574.8
Electrophoresis Power Supply
LA690-1NO
EWC Diagnostics
1 unit
EUR 432.84
Description: Electrophoresis Power Supply
ES 6 Tooth Gel Comb For ES Electrophoresis
LE1011-A4
GenDepot
Ea
EUR 70
HiPer® Agarose Gel Electrophoresis Teach
HTBM001-10PR
EWC Diagnostics
1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach
Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611
Bio Basic
1 UNIT
EUR 383.11
Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613
Bio Basic
1 UNIT
EUR 487.51
Glycine for electrophoresis, 99.5%
52574
Sisco Laboratories
100 Gms
EUR 9.58
Description: Part A
ELECTROPHORESIS PS 1200V, 500MA, 300W 120 VAC
EV3150-US
Consort
ea
EUR 1060
ELECTROPHORESIS PS 3000V, 200MA, 300W 120 VAC
EV3330-US
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W 120 VAC
EV3620-US
Consort
ea
EUR 1600
Agarose for DNA electrophoresis
16-0027-500
Medicago
500 g
EUR 410.4
10X MOPS Electrophoresis Buffer
ML050-500ML
EWC Diagnostics
1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer
ELECTROPHORESIS PS 600V, 500MA, 150W 120VAC
EV2650-US
Consort
ea
EUR 750
CometAssay Electrophoresis System
4250-050-ES
Biotechne
1 Kit
EUR 3540.96
ELECTROPHORESIS PS 300V, 1000MA, 150W 120 VAC
EV2310-US
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 2000MA, 300W 120 VAC
EV3020-US
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W 120 VAC
EV3610-US
Consort
ea
EUR 1060
SDS-PAGE Electrophoresis Buffer
MBS355536-1L
MyBiosource
1L
EUR 165
SDS-PAGE Electrophoresis Buffer
MBS355536-5x1L
MyBiosource
5x1L
EUR 480
SDS-PAGE Electrophoresis Buffer
MBS355542-10x1L
MyBiosource
10x1L
EUR 250
6X6 well Electrophoresis Apparatus
EDK626
Bio Basic
1 UNIT
EUR 750.6
CometAssay Single Cell Gel Electrophoresis Assay
4250-050-K
Biotechne
50 tests
EUR 513.6
electrophoresis power supply 120 vac
EV245-US
Consort
ea
EUR 574.8
10-well protein Vertical Electrophoresis Apparatus
EDK692
Bio Basic
1 UNIT
EUR 487.51
Aminoacetic acid, ?For electrophoresis
GRM2233-100G
EWC Diagnostics
1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis
Aminoacetic acid, ?For electrophoresis
GRM2233-500G
EWC Diagnostics
1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis
electrophoresis power supply uk plug
EV245-UK
Consort
ea
EUR 574.8
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)
EDK614
Bio Basic
1 UNIT
EUR 520.92
myGel Mini Electrophoresis System, 115V
BM0286
GenDepot
System
EUR 560
CometAssay Electrophoresis Starter Kit
4250-050-ESK
Biotechne
1 Kit
EUR 3749.28
Gel Electrophoresis System with Time Control, 220-240 V
Q-EP-2114
ABM
1
EUR 1180
Agarose, medium EEO ?For electrophoresis
RM6249-100G
EWC Diagnostics
1 unit
EUR 119.06
Description: Agarose, medium EEO ?For electrophoresis
Agarose, medium EEO ?For electrophoresis
RM6249-10G
EWC Diagnostics
1 unit
EUR 19.63
Description: Agarose, medium EEO ?For electrophoresis
Agarose, medium EEO ?For electrophoresis
RM6249-1KG
EWC Diagnostics
1 unit
EUR 1089.43
Description: Agarose, medium EEO ?For electrophoresis
Agarose, medium EEO ?For electrophoresis
RM6249-25G
EWC Diagnostics
1 unit
EUR 36.87
Description: Agarose, medium EEO ?For electrophoresis
Alkaline Hemoglobin Electrophoresis Kit
MBP001-112RUNS
EWC Diagnostics
1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Hemoglobin Electrophoresis Kit
MBP001-224RUNS
EWC Diagnostics
1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Hemoglobin Electrophoresis Kit
MBP008-224RUNS
EWC Diagnostics
1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Paper Electrophoresis Kit (Cell
MBP009-20RUNS
EWC Diagnostics
1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell
WEE VERT PROTEIN ELECTROPHORESIS SYSTEM
LA1070-1NO
EWC Diagnostics
1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM
Electrophoresis Power Supply (2 termina
LA842-1NO
EWC Diagnostics
1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina
Cellulose Acetate Electrophoresis System
LA1027-1NO
EWC Diagnostics
1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-1
Bio-WORLD
15 mL
EUR 21.21
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-2
Bio-WORLD
25 mL
EUR 28.49
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-3
Bio-WORLD
50 mL
EUR 43.44
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-4
Bio-WORLD
100 mL
EUR 77.25
20X DoubleQuick DNA Electrophoresis Buffer
73530
Sisco Laboratories
250 ml
EUR 35.58
Description: Part E
20X DoubleQuick DNA Electrophoresis Buffer
73530-1
Sisco Laboratories
500 ml
EUR 64.4
Description: Part E
Gel Electrophoresis System with Muticaster, 100-120 V Input
Q-EP-2034
ABM
1
EUR 1715
Gel Electrophoresis System with Muticaster, 220-240 V Input
Q-EP-2056
ABM
1
EUR 2125
Gel Electrophoresis System with Time Control, 100-120 V Input
Q-EP-2100
ABM
1
EUR 1385
CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K
Biotechne
1 kit
EUR 684.96
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-005mL
MyBiosource
0.05mL
EUR 195
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-01mL
MyBiosource
0.1mL
EUR 255
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-5x005mL
MyBiosource
5x0.05mL
EUR 820
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-5x01mL
MyBiosource
5x0.1mL
EUR 1100
Ammonium Persulphate (APS) for electrophoresis, 99%
28575
Sisco Laboratories
25 Gms
EUR 1.51
Description: Part A
Tris-HEPES-SDS Buffer 20X for Electrophoresis
20840057-1
Bio-WORLD
500 mL
EUR 91.56
Tris-HEPES-SDS Buffer 20X for Electrophoresis
20840057-2
Bio-WORLD
1 L
EUR 173.27
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380
ACT-CBMN-7
ACTGene
each
EUR 892.8
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-005mL
MyBiosource
0.05mL
EUR 180
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-01mL
MyBiosource
0.1mL
EUR 230
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-05mL
MyBiosource
0.5mL
EUR 695
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-5x005mL
MyBiosource
5x0.05mL
EUR 760
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-5x01mL
MyBiosource
5x0.1mL
EUR 980
SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F007-100-uL
EnoGene
100 μL
EUR 227
Description: Reagents for ssDNA or RNA electrophoresis
SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F007-50-uL
EnoGene
50 μL
EUR 133
Description: Reagents for ssDNA or RNA electrophoresis
myGel Mini Electrophoresis System 230V (includes transformer)
BM0291
GenDepot
System
EUR 580
myGel Mini Electrophoresis System 230V (includes transformer)
E1101-E
Benchmark Scientific
1 PC
EUR 665.55
CometAssay Electrophoresis Starter Kit plus EU Power Supply
4250-050-ESK-PS2
Biotechne
1 Kit
EUR 4597.68
CometAssay Electrophoresis Starter Kit plus UK Power Supply
4250-050-ESK-PS3
Biotechne
1 Kit
EUR 4597.68
CometAssay Electrophoresis Starter Kit plus SZ Power Supply
4250-050-ESK-PS4
Biotechne
1 Kit
EUR 4597.68
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-100-uL
EnoGene
100 μL
EUR 227
Description: Reagents for dsDNA electrophoresis
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-500-uL
EnoGene
500 μL
EUR 983
Description: Reagents for dsDNA electrophoresis
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-50uL
EnoGene
50 μL
EUR 133
Description: Reagents for dsDNA electrophoresis
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3231
EnoGene
50T
EUR 100
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3232
EnoGene
100T
EUR 150
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)
E1WP326
EnoGene
50T
EUR 100
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)
E1WP324
EnoGene
50T
EUR 60
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator
E1201
Benchmark Scientific
1 each
EUR 1001.7
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)
E1101-SK
Benchmark Scientific
1 each
EUR 727.06
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)
E1101-SK-E
Benchmark Scientific
1 PC
EUR 757.35
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input
E1201-E
Benchmark Scientific
1 each
EUR 1058.9
OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH
VS10WCES
Scientific Laboratory Supplies
EACH
EUR 1614.21
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)
E37F007
EnoGene
100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis
SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)
E37F001
EnoGene
100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis
AXYGEN® HORIZONTAL GEL BOX, 10 CM
HGB-10
CORNING
1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ
Horizontal head, for 12 x 1.5ml
BCM1604
Bio Basic
1 pcs, 1 UNIT
EUR 200.68
Taken together, these findings indicate that our method allows semi-quantitative analysis to estimate the ratio of human antibodies compared with the IC included in the total antibody. This method was successfully applied to the in vivo studies using rats, and the data helps explain the unexpectedly rapid clearance of human antibodies for the formation of large IC. The combination of IC separation by BN-PAGE and detection of peptide-specific human IgG with LC-MS / MS bioanalytical approach is common, useful for detecting IC in animals.