Medaka Sea is one of the most popular models of fish species for Ecotoxicology and environmental research and study of proteomics is a useful tool for understanding the medaka molecular response after exposure to different environmental stresses. High-quality protein sample preparation is the key to producing high-quality two-dimensional gel electrophoresis (2-DE) results for proteomics analysis.
In recent years, the extraction of protein-based Trizol has gained popularity because of its performance promise in producing high-quality 2-DE and comfort method.Three Trizol-based approach (Trizol method, the method and the method of Trizol Trizol aliquots with commercial cleaning kit) is used to extract protein from marine samples medaka and 2-DE profiles produced. 2-DE profile quality and effectiveness of extraction methods were evaluated.
For comparison, two common protein extraction method (method of lysis buffer and trichloroacetic acid (TCA) / acetone precipitation extraction) are also applied in parallel to Trizol based approaches.Any three Trizol-based approach produces high-quality 2-DE profiles of marine medaka compared with both methods of lysis buffer and TCA / acetone precipitation extraction.
In addition, the Trizol method with clean-up kit produced the best commercial 2-DE profile in terms of the clarity of the background, the number of spots and resolution proteins.Trizol based approach offered a better choice than traditional protein extraction method for 2-DE analysis of medaka sea. A modified version of the method Trizol with commercial clean-up kit was shown to produce the best 2-DE profile.

A method of combining blue native polyacrylamide gel electrophoresis liquid chromatography-tandem mass spectrometry for the detection of circulating immune complexes between the monoclonal antibody therapy and anti-drug antibodies in animals.
Monoclonal antibody therapy can potentially induce unwanted immune response, so that the production of anti-drug antibodies (ADAS). Binding of fennel drug and subsequent formation of immune complexes (ICs) can trigger a variety of responses, depending on the size, concentration, and a subclass of fennel. To better understand the impact of fennel in pharmacokinetics, pharmacodynamics and toxicology profile, bioanalytical methods developed to detect IC between a human monoclonal immunoglobulin G (IgG) and fennel in a biological sample.
Regarding the experimental procedure, in brief, human-specific antibodies and human antibodies bound IC in biological samples separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The target fraction is then cut from the gel, followed by in-gel trypsin digestion and subsequent liquid chromatography-tandem mass spectrometry (LC-MS / MS) to monitor the human peptide-specific IgG. This method is capable of detecting various types of human antibodies with a lower detection limit of 10 ug / mL in monkey serum. Performance IC assay to detect the expressed using spiked samples, and pre-incubated in monkey serum IC clearly detectable.
5 gel caster for 8 x 10cm or 10 x 10cm gels. |
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CSL6CAST | Scientific Laboratory Supplies | EACH | EUR 508.44 |
ES Horizontal Electrophoresis Set |
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LE1011 | GenDepot | Set | EUR 578.4 |
Dummy Plate 10 x 10cm |
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VS10DP | Scientific Laboratory Supplies | EACH | EUR 23.94 |
20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System |
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LE1000C10-10 | GenDepot | Ea | EUR 153.6 |
10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System |
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LE1000C10-01 | GenDepot | Ea | EUR 153.6 |
10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System |
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LE1000C10-02 | GenDepot | Ea | EUR 153.6 |
AXYGEN® HORIZONTAL GEL BOX, 10 CM |
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HGB-10 | CORNING | 1/pk | EUR 399.6 |
Description: Lab Equipment; Axygen Branded EQ |
Casting Dams, For Enduro 10cm Gel System |
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LE1000CD-02 | GenDepot | Ea | EUR 165.6 |
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs |
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ELE2302 | Scientific Laboratory Supplies | EACH | EUR 1520.76 |
Multi Sub Midi 10 x 10cm UV Tray |
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MS10UV10 | Scientific Laboratory Supplies | EACH | EUR 79.8 |
Vert Sub Mini 10 x 10cm Dual Caster |
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VS10DCAST | Scientific Laboratory Supplies | EACH | EUR 196.08 |
Replacement Rubber mats for 10 x 10cm caster |
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VS10DCASTM | Scientific Laboratory Supplies | EACH | EUR 50.16 |
Burnsoothe Burn Relief Dressing 10cm x 10cm |
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SAF1294 | Scientific Laboratory Supplies | PK10 | EUR 47.88 |
CoolTherm burn relief dressing 10cm x 10cm |
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SAF1330 | Scientific Laboratory Supplies | PK10 | EUR 164.16 |
99445-10 DCT 10 X 75MM |
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99445-10 | CORNING | 250/pk | EUR 75.6 |
Description: Disposable Culture Tubes; DCT's, CGW |
6 x 15ml horizontal rotisserie |
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R4040-HZ15 | Benchmark Scientific | 1 PC | EUR 127.76 |
6 x 50ml horizontal rotisserie |
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R4040-HZ50 | Benchmark Scientific | 1 PC | EUR 197.63 |
5 x SDS Electrophoresis Buffer |
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GR103039 | Genorise Scientific | 1 L | EUR 128.4 |
C18 3um 10cm x 3mm |
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CHR4804 | Scientific Laboratory Supplies | EACH | EUR 644.1 |
AQ 3um 10cm x 2.1mm |
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CHR4990 | Scientific Laboratory Supplies | EACH | EUR 664.62 |
AQ 5um 10cm x 4.6mm |
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CHR4992 | Scientific Laboratory Supplies | EACH | EUR 607.62 |
GC18 4um 10cm x 4.6mm |
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CHR5004 | Scientific Laboratory Supplies | EACH | EUR 579.12 |
Dummy Plate 20 x 10cm |
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VS10WDP | Scientific Laboratory Supplies | EACH | EUR 26.22 |
12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System |
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LE1000C10-03 | GenDepot | Ea | EUR 153.6 |
12 Well Comb ,1mm Thick For Enduro 10cm Gel System |
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LE1000C10-04 | GenDepot | Ea | EUR 153.6 |
12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System |
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LE1000C10-05 | GenDepot | Ea | EUR 153.6 |
16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System |
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LE1000C10-06 | GenDepot | Ea | EUR 153.6 |
16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System |
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LE1000C10-07 | GenDepot | Ea | EUR 153.6 |
16 Well Comb ,2mm Thick For Enduro 10cm Gel System |
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LE1000C10-08 | GenDepot | Ea | EUR 153.6 |
25 Well Comb ,1mm Thick For Enduro 10cm Gel System |
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LE1000C10-11 | GenDepot | Ea | EUR 153.6 |
8 Well Comb ,1mm Thick For Enduro 10cm Gel System |
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LE1000C10-12 | GenDepot | Ea | EUR 153.6 |
10 x 10cm Notched Glass Plates 2mm thick Pack of 2 |
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VS10NG | Scientific Laboratory Supplies | PK2 | EUR 38.76 |
Horizontal head, for 12 x 1.5ml |
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BCM1604 | Bio Basic | 1 pcs, 1 UNIT | EUR 200.68 |
Horizontal head, for 4 x 15ml |
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BCM1605 | Bio Basic | 1 pcs, 1 UNIT | EUR 200.68 |
Horizontal head, for 2 x 50ml |
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BCM1606 | Bio Basic | 1 pcs, 1 UNIT | EUR 200.68 |
Horizontal head for 12 x 1.5mL |
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MIX7018 | Scientific Laboratory Supplies | EACH | EUR 121.98 |
Horizontal head for 4 x 15mL |
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MIX7020 | Scientific Laboratory Supplies | EACH | EUR 121.98 |
Horizontal head for 2 x 50mL |
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MIX7022 | Scientific Laboratory Supplies | EACH | EUR 121.98 |
4 x Phosphate/SDS Electrophoresis Buffer |
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GR103033 | Genorise Scientific | 1 L | EUR 139.2 |
SU C18 3.5u 10cm x 3.0mm |
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CHR4376 | Scientific Laboratory Supplies | EACH | EUR 687.35 |
C18 5um 10cm x 30mm id |
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CHR4826 | Scientific Laboratory Supplies | EACH | EUR 4822.2 |
C18 Polar 3u 10cm x 2.1mm |
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CHR4864 | Scientific Laboratory Supplies | EACH | EUR 643.61 |
MSP-C4 5um 10cm x 2.1mm |
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CHR5014 | Scientific Laboratory Supplies | EACH | EUR 593.62 |
MSP-C4 5um 10cm x 3.0mm |
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CHR5016 | Scientific Laboratory Supplies | EACH | EUR 593.62 |
MSP-C4 5um 10cm x 4.0mm |
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CHR5018 | Scientific Laboratory Supplies | EACH | EUR 593.62 |
MDC-C18 5um 10cm x 3.0mm |
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CHR5048 | Scientific Laboratory Supplies | EACH | EUR 612.36 |
MDC-C18 5um 10cm x 4.0mm |
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CHR5050 | Scientific Laboratory Supplies | EACH | EUR 612.36 |
MDC-C18 5um 10cm x 4.6mm |
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CHR5052 | Scientific Laboratory Supplies | EACH | EUR 612.36 |
Electrophoresis Apparatus - 7x10cm (6-10-well) |
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EDK611 | Bio Basic | 1 UNIT | EUR 383.11 |
Electrophoresis Apparatus - 7x14cm (6-10-well) |
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EDK613 | Bio Basic | 1 UNIT | EUR 487.51 |
10-well protein Vertical Electrophoresis Apparatus |
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EDK692 | Bio Basic | 1 UNIT | EUR 487.51 |
20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System |
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LE1000C10-09 | GenDepot | Ea | EUR 153.6 |
Kinesis Tubing PEEKsil 1/32 x 25um x 10cm |
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CHR2453 | Scientific Laboratory Supplies | PK2 | EUR 43.32 |
Kinesis TuBING PEEKsil 1/32 x 50um x 10cm |
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CHR2463 | Scientific Laboratory Supplies | PK2 | EUR 43.32 |
Kinesis Tubing PEEKsil 1/16 x 25um x 10cm |
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CHR2593 | Scientific Laboratory Supplies | PK5 | EUR 110.58 |
Kinesis Tubing PEEKsil 1/16 x 50um x 10cm |
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CHR2611 | Scientific Laboratory Supplies | PK5 | EUR 102.6 |
Kinesis Tub SS 1/32 x 205um x 10cm |
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CHR4823 | Scientific Laboratory Supplies | EACH | EUR 13.68 |
Kinesis Tub SS 1/32 x .015 x 10cm |
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CHR4841 | Scientific Laboratory Supplies | EACH | EUR 20.52 |
6 x 15mL horizontal rotisserie 1 ea |
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MIX7080 | Scientific Laboratory Supplies | EACH | EUR 76.38 |
6 x 50mL horizontal rotisserie 1 ea |
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MIX7082 | Scientific Laboratory Supplies | EACH | EUR 76.38 |
ES Electrophoresis Casting Tray, 7cm X 14cm |
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LE1011-A1 | GenDepot | Ea | EUR 198 |
ES Electrophoresis Casting Tray, 7cm X 7cm |
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LE1011-A2 | GenDepot | Ea | EUR 181.2 |
CoolTherm burn relief dressing 10cm x 40cm |
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SAF1332 | Scientific Laboratory Supplies | PK7 | EUR 152.76 |
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs |
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ELE2300 | Scientific Laboratory Supplies | EACH | EUR 1448.94 |
midiplus horizontal unit 15x7-10-15 cm |
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EHS1300-SYS | Consort | ea | EUR 496.8 |
Genorise® 10 x RNA Sample Loading Buffer |
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GR102010-10 | Genorise Scientific | 10 mL | EUR 177.6 |
Nitrocellulose Membrane, 0.25 µm, 9 cm x 10cm |
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AR0135-02 | BosterBio | 20 sheets/Pack | EUR 115.2 |
Nitrocellulose Membrane, 0.45 µm, 9 cm x 10cm |
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AR0135-04 | BosterBio | 20 sheets/Pack | EUR 115.2 |
Multi Sub Choice 15 x 10cm UV Tray |
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MS15UV10 | Scientific Laboratory Supplies | EACH | EUR 93.48 |
Multi Sub Maxi 20 x 10cm UV Tray |
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MS20UV10 | Scientific Laboratory Supplies | EACH | EUR 124.26 |
Multi Sub Mini 7 x 10cm UV Tray |
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MS7UV10 | Scientific Laboratory Supplies | EACH | EUR 72.96 |
LU C18 (2) 3um 10cm x 3mm id |
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CHR3964 | Scientific Laboratory Supplies | EACH | EUR 643.61 |
Horizontal head |
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BV1000-H15 | Benchmark Scientific | 1 PC | EUR 161.26 |
Horizontal head |
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BV1000-H150 | Benchmark Scientific | 1 PC | EUR 161.26 |
Horizontal head |
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BV1000-H500 | Benchmark Scientific | 1 PC | EUR 2813.28 |
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well) |
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EDK614 | Bio Basic | 1 UNIT | EUR 520.92 |
Daunomycin (10.10), DNA Aptamer, Biotinylated |
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AD-113-B | Alpha Diagnostics | Custom | Ask for price |
Daunomycin (10.10), DNA Aptamer, unlabeled |
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AD-113-U | Alpha Diagnostics | Custom | Ask for price |
Technical Buffer pH 10.10 250ml |
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51350010 | Scientific Laboratory Supplies | EACH | EUR 25.99 |
MAGic Clamp Tube Rack 4 x 15mL tubes horizontal |
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MIX7174 | Scientific Laboratory Supplies | EACH | EUR 133.38 |
MAGic Clamp Tube Rack 2 x 50mL tubes horizontal |
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MIX7176 | Scientific Laboratory Supplies | EACH | EUR 133.38 |
Kinesis Tubing SS 1/16od x 0.02id - 10cm Length |
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CHR4789 | Scientific Laboratory Supplies | EACH | EUR 5.7 |
Kinesis Tubing SS 1/32 x 0.018 - 10cm Length |
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CHR4849 | Scientific Laboratory Supplies | EACH | EUR 20.52 |
Kinesis Tubing SS 1/16od x 0.03id - 10cm Length |
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CHR4855 | Scientific Laboratory Supplies | EACH | EUR 7.98 |
Kinesis Tubing SS 1/16 x .040 - 10cm Length |
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CHR4887 | Scientific Laboratory Supplies | EACH | EUR 10.26 |
Kinesis Tubing SS 1/16 x .046 - 10cm Length |
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CHR4901 | Scientific Laboratory Supplies | EACH | EUR 11.4 |
AXYGEN® GEL TRAY 10 X 10 CM FOR USE WITH 10 CM GEL BOX, UV TRANSPARENT |
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HGB10-10-GT | CORNING | 1/pk | EUR 88.8 |
Description: Lab Equipment; Axygen Branded EQ |
ES 8/10 Double Gel Comb For ES Electrophoresis |
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LE1011-A3 | GenDepot | Ea | EUR 148.8 |
GELS Polyclonal Antibody |
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ES11007-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against GELS from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
GELS Polyclonal Antibody |
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ES11007-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against GELS from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
GELS Polyclonal Antibody |
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ABP58629-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of GELS from Human, Mouse, Rat. This GELS antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GELS protein |
GELS Polyclonal Antibody |
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ABP58629-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of GELS from Human, Mouse, Rat. This GELS antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GELS protein |
GELS Polyclonal Antibody |
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ABP58629-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of GELS from Human, Mouse, Rat. This GELS antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GELS protein |
Anti-GELS antibody |
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STJ192165 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to GELS |
20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System |
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LE1000C15-10 | GenDepot | Ea | EUR 157.2 |
50 Well Comb ,2mm Thick For Enduro 20cm Gel System |
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LE1000C20-10 | GenDepot | Ea | EUR 165.6 |
Gel Tray 10 X 10 Cm |
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LE1000GT-03 | GenDepot | Ea | EUR 170.4 |
ENDURO™ PowerStack |
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LE0304S | GenDepot | Ea | EUR 724.8 |
Horizontal MicroLoops E |
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M8-L18SP-15H | MiTeGen | 20 MOUNTS | EUR 112 |
Description: Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods |
Horizontal MicroLoops E |
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M8-L18SP-50H | MiTeGen | 20 MOUNTS | EUR 112 |
Description: Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods |
Daunomycin (10.10), DNA Aptamer, FITC labeled |
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AD-113-F | Alpha Diagnostics | Custom | Ask for price |
electrophoresis power supply |
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EV245 | Consort | ea | EUR 574.8 |
CometAssay Electrophoresis System |
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4250-050-ES | Biotechne | 1 Kit | EUR 3540.96 |
miniONE Electrophoresis System |
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ELE3400 | Scientific Laboratory Supplies | EACH | EUR 624.72 |
MAGic Clamp Tube Rack 12 x 1.5/2.0mL tubes horizontal |
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MIX7172 | Scientific Laboratory Supplies | EACH | EUR 133.38 |
TBE Bufer, 10 x |
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GR103009 | Genorise Scientific | 1 L | EUR 99.6 |
TBST Bufer, 10 x |
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GR103010 | Genorise Scientific | 1 L | EUR 128.4 |
TE Bufer, 10 x |
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GR103012 | Genorise Scientific | 500 mL | EUR 190.8 |
Silicon Septa x 10 |
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WAT4826 | Scientific Laboratory Supplies | PK10 | EUR 144.78 |
MultiBox for Western blotting, 4 compartments, 38 x 71 x 28mm each (11/2 x 2 13/16 x 11/8 in.), for various gels including half-mini protein gels |
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B1204 | MTC Bio | 6/pack | EUR 119.14 |
Description: Western Blot Boxes |
Kinesis Tubing SS 1/32 x 250um (0.010) - 10cm Length |
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CHR4831 | Scientific Laboratory Supplies | EACH | EUR 21.66 |
Kinesis Tubing SS 1/16 x 180um(.007in) - 10cm Length |
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CHR4863 | Scientific Laboratory Supplies | EACH | EUR 6.84 |
Kinesis Tubing SS 1/16 x 125um(.005in) - 10cm Length |
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CHR4915 | Scientific Laboratory Supplies | EACH | EUR 6.84 |
9 x 10cm Notched Glass Plates with 0.75mm Bonded Spacers |
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VS10NGS075 | Scientific Laboratory Supplies | EACH | EUR 53.58 |
9 x 10cm Plain Glass Plates with 0.75mm Bonded Spacers |
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VS10PGS075 | Scientific Laboratory Supplies | EACH | EUR 35.34 |
19 x 10cm Notched Glass Plates with 0.75mm Bonded Spacers |
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VS10WNGS075 | Scientific Laboratory Supplies | EACH | EUR 125.4 |
19 x 10cm Notched Glass Plates with 1mm Bonded Spacers |
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VS10WNGS1 | Scientific Laboratory Supplies | EACH | EUR 132.24 |
Accuris Gel Casting Set for 5x6cm Gels (18/10 Teeth) |
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ELE1114 | Scientific Laboratory Supplies | EACH | EUR 86.64 |
10 Well Comb ,1mm Thick For Enduro 15cm Gel System |
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LE1000C15-01 | GenDepot | Ea | EUR 157.2 |
10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System |
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LE1000C15-02 | GenDepot | Ea | EUR 157.2 |
10 Well Comb , 1mm Thick For Enduro 7cm Gel System |
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LE1000C7-01 | GenDepot | Ea | EUR 148.8 |
10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System |
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LE1000C7-02 | GenDepot | Ea | EUR 148.8 |
Horizontal outer dims 415h x 140w x 560d with 11 apertures of approx 37mm |
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AH17911 | Scientific Laboratory Supplies | EACH | EUR 149.34 |
Horizontal outer dims 415h x 140w x 560d with 6 apertures of approx 67mm |
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AH1796UT | Scientific Laboratory Supplies | EACH | EUR 168.72 |
X-NeuNAc |
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A3944-10 | ApexBio | 10 mg | EUR 157.2 |
Description: Km: 0.89 mM for neuraminidase X-Neu5Ac is a new substrate for chromogenic assay of neuraminidase activity in bacterial expression systems. |
Tenacissoside X |
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N2110-10 | ApexBio | 10 mg | EUR 686.4 |
Description: Extracted from Marsdenia tenacissima (Roxb.) Wight et Arn.;Store the product in sealed, cool and dry condition |
Platform for 10 x 10 mL tubes |
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MIX1256 | Scientific Laboratory Supplies | EACH | EUR 47.88 |
Digital Hotplate, 10 x 10 inch, 115V |
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H3710-H | Benchmark Scientific | 1 each | EUR 545.3 |
Digital Hotplate, 10 x 10 inch, 230V |
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H3710-H-E | Benchmark Scientific | 1 each | EUR 545.3 |
Magnetic Stirrer, 10 x 10 inch, 115V |
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H3710-S | Benchmark Scientific | 1 each | EUR 545.3 |
Magnetic Stirrer, 10 x 10 inch, 230V |
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H3710-SE | Benchmark Scientific | 1 each | EUR 545.3 |
DIGE gel 3 gels |
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28937451 | Scientific Laboratory Supplies | EACH | EUR 486.78 |
1730 10 SNAP-SEAL 10 OZ |
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1730-10 | CORNING | 100/pk | EUR 96 |
Description: Disposable Plastic; Plastic Containers |
9 x 10cm Plain Glass Plates 2mm thick Pack of 2 |
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VS10PG | Scientific Laboratory Supplies | EACH | EUR 28.5 |
GSA 10 |
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B5767-10 | ApexBio | 10 mg | EUR 274.8 |
SC-10 |
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B6299-10 | ApexBio | 10 mg | EUR 255.6 |
MRT 10 |
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B7685-10 | ApexBio | 10 mg | EUR 285.6 |
Kodak X-Ray Film 8 x 10 inch |
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B503 | ABM | 100 Sheets | EUR 242.4 |
Horizontal 50mL Tube Holder |
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MIX5088 | Scientific Laboratory Supplies | EACH | EUR 144.78 |
Pipette Tray PVC Horizontal |
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PIP5500 | Scientific Laboratory Supplies | EACH | EUR 38.76 |
Midi Horizontal Gel Unit |
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ELE8120 | Scientific Laboratory Supplies | EACH | EUR 630.42 |
Horizontal orientator 384 wells |
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DD53423 | Scientific Laboratory Supplies | PK2 | EUR 63.84 |
10 x Cell Lysis Buffer |
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GR103003x10 | Genorise Scientific | 200 mL | EUR 141.6 |
10 x PBS, pH 7.30 |
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GR103004x10 | Genorise Scientific | 1 L | EUR 100.8 |
10 x Universal Blocking Buffer |
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GR103005 | Genorise Scientific | 200 mL | EUR 154.8 |
10 x ELISA Wash Buffer |
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GR103014 | Genorise Scientific | 1 L | EUR 117.6 |
10 x ELISA Reagent Diluent |
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GR103028 | Genorise Scientific | 100 mL | EUR 92.4 |
Hypercassette Neutral 10 X 12In |
|||
RPN11650 | Scientific Laboratory Supplies | EACH | EUR 367.08 |
4 X 10 CARBON CARTRIDGE |
|||
CDFC410F2 | Scientific Laboratory Supplies | EACH | EUR 295.26 |
Bellows pasteur pipette x 10 |
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WAT4772 | Scientific Laboratory Supplies | PK10 | EUR 144.78 |
CometAssay Electrophoresis Starter Kit |
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4250-050-ESK | Biotechne | 1 Kit | EUR 3749.28 |
Agarose for DNA electrophoresis |
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16-0027-500 | Medicago | 500 g | EUR 410.4 |
myGel Mini Electrophoresis System |
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BM0286 | GenDepot | System | EUR 741.6 |
Acrylamide, suitable for electrophoresis |
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GK5651-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Starch (Smithies), for electrophoresis |
|||
GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 370.8 |
Starch (Smithies), for electrophoresis |
|||
GE6857-500G | Glentham Life Sciences | 500 g | EUR 217.2 |
6X6 well Electrophoresis Apparatus |
|||
EDK626 | Bio Basic | 1 UNIT | EUR 750.6 |
10cm UV Gel Scoop |
|||
MS10UVS | Scientific Laboratory Supplies | EACH | EUR 51.3 |
Chiller for Electrophoresis systems |
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CSLCHILLER | Scientific Laboratory Supplies | EACH | EUR 6453.54 |
myGel Mini Electrophoresis System |
|||
E1101 | Benchmark Scientific | 1 each | EUR 623.9 |
Kinesis Adapt - 10-32M x 10-32F - PEEK |
|||
CHR4283 | Scientific Laboratory Supplies | EACH | EUR 27.36 |
AI-10-49 |
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A8694-10 | ApexBio | 10 mg | EUR 254.4 |
Description: AI-10-49 is a selective inhibitor of CBF? -SMMHC and RUNX1 interaction with a FRET IC50 value of 260nM. AI-10-49 restores RUNX1 transcriptional activity, displays favorable pharmacokinetics, and delays leukemia progression in mice. |
Keratin 10 antibody |
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10-2541 | Fitzgerald | 250 ug | EUR 590.4 |
Description: Mouse monoclonal Keratin 10 antibody |
MFZ 10-7 |
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B5595-10 | ApexBio | 10 mg | EUR 420 |
TC ASK 10 |
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B5728-10 | ApexBio | 10 mg | EUR 525.6 |
PSB 10 hydrochloride |
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B6923-10 | ApexBio | 10 mg | EUR 439.2 |
10-DEBC hydrochloride |
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B7105-10 | ApexBio | 10 mg | EUR 265.2 |
Description: 10-DEBC hydrochloride is a selective inhibitor of Akt (or termed PKB) [1], with an IC50 value of approximate 48 ?M [2].Akt is a type of serine/threonine kinase. It phosphorylates and inactivates components in the apoptotic machinery, including Caspase 9 and BAD. |
KinaSorb™ 10 |
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KE785-10 | Biotech Support Group | 10 preps | EUR 553.2 |
Empty Tubes (10) |
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2MLET-10 | BioAssay Systems | 10 | EUR 17 |
Description: Empty tubes used for preparation of urine samples for use with the Oxalate Quick Test Strips. Quantity: 10 empty 2mL tubes. |
Aluminum Test Tube Basket 10 x 6 x 6 |
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HS20341B | Scientific Laboratory Supplies | EACH | EUR 40.47 |
Finntip 10 0.2-10ul Pink 10 x 96 rack |
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PIP3644 | Scientific Laboratory Supplies | PK960 | EUR 111.72 |
Cytiva ALUMINA PLATES 10 X 8 CM 10 EA |
|||
GESE202N10 | Scientific Laboratory Supplies | PK10 | EUR 327.18 |
Aluminium Tray For 10 X 10 Wide Vials 28.5mm |
|||
FLY1320 | Scientific Laboratory Supplies | EACH | EUR 93.48 |
Taken together, these findings indicate that our method allows semi-quantitative analysis to estimate the ratio of human antibodies compared with the IC included in the total antibody. This method was successfully applied to the in vivo studies using rats, and the data helps explain the unexpectedly rapid clearance of human antibodies for the formation of large IC. The combination of IC separation by BN-PAGE and detection of peptide-specific human IgG with LC-MS / MS bioanalytical approach is common, useful for detecting IC in animals.