Medaka Sea is one of the most popular models of fish species for Ecotoxicology and environmental research and study of proteomics is a useful tool for understanding the medaka molecular response after exposure to different environmental stresses. High-quality protein sample preparation is the key to producing high-quality two-dimensional gel electrophoresis (2-DE) results for proteomics analysis.
In recent years, the extraction of protein-based Trizol has gained popularity because of its performance promise in producing high-quality 2-DE and comfort method.Three Trizol-based approach (Trizol method, the method and the method of Trizol Trizol aliquots with commercial cleaning kit) is used to extract protein from marine samples medaka and 2-DE profiles produced. 2-DE profile quality and effectiveness of extraction methods were evaluated.
For comparison, two common protein extraction method (method of lysis buffer and trichloroacetic acid (TCA) / acetone precipitation extraction) are also applied in parallel to Trizol based approaches.Any three Trizol-based approach produces high-quality 2-DE profiles of marine medaka compared with both methods of lysis buffer and TCA / acetone precipitation extraction.
In addition, the Trizol method with clean-up kit produced the best commercial 2-DE profile in terms of the clarity of the background, the number of spots and resolution proteins.Trizol based approach offered a better choice than traditional protein extraction method for 2-DE analysis of medaka sea. A modified version of the method Trizol with commercial clean-up kit was shown to produce the best 2-DE profile.
A method of combining blue native polyacrylamide gel electrophoresis liquid chromatography-tandem mass spectrometry for the detection of circulating immune complexes between the monoclonal antibody therapy and anti-drug antibodies in animals.
Monoclonal antibody therapy can potentially induce unwanted immune response, so that the production of anti-drug antibodies (ADAS). Binding of fennel drug and subsequent formation of immune complexes (ICs) can trigger a variety of responses, depending on the size, concentration, and a subclass of fennel. To better understand the impact of fennel in pharmacokinetics, pharmacodynamics and toxicology profile, bioanalytical methods developed to detect IC between a human monoclonal immunoglobulin G (IgG) and fennel in a biological sample.
Regarding the experimental procedure, in brief, human-specific antibodies and human antibodies bound IC in biological samples separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The target fraction is then cut from the gel, followed by in-gel trypsin digestion and subsequent liquid chromatography-tandem mass spectrometry (LC-MS / MS) to monitor the human peptide-specific IgG. This method is capable of detecting various types of human antibodies with a lower detection limit of 10 ug / mL in monkey serum. Performance IC assay to detect the expressed using spiked samples, and pre-incubated in monkey serum IC clearly detectable.
ES Horizontal Electrophoresis Set |
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LE1011 | GenDepot | Set | EUR 578.4 |
Hi-Eco Mini Horizontal Electrophoresis K |
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LA851-1NO | EWC Diagnostics | 1 unit | EUR 205.56 |
Description: Hi-Eco Mini Horizontal Electrophoresis K |
ComboMiniTM Horizontal Electrophoresis System (110V) |
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CBMN-7 | Avans Biotechnology Corp | each | EUR 500 |
ComboMiniTM Horizontal Electrophoresis System (220V) |
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CBMN-8 | Avans Biotechnology Corp | each | EUR 530 |
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH |
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ELE2302 | Scientific Laboratory Supplies | EACH | EUR 1891.35 |
WSHT-miniDNA Mini Horizontal Electrophoresis Cell |
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HTES1008 | Shanghai WSHT Biotechnology | set | EUR 193.2 |
WSHT-liteDNA Universal Horizontal Electrophoresis Cell |
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HTES1007 | Shanghai WSHT Biotechnology | set | EUR 259 |
WSHT-midiDNA Multipurpose Horizontal Electrophoresis Cell |
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HTES1006 | Shanghai WSHT Biotechnology | set | EUR 278.6 |
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH |
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ELE2300 | Scientific Laboratory Supplies | EACH | EUR 1802.25 |
Electrophoresis Module for Eco-Mini, for 2 gels |
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AJ846-017-175 | Westburg | each | EUR 604.95 |
Electrophoresis Module for Eco-Maxi, for 2 gels |
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AJ846-017-475 | Westburg | each | EUR 997.35 |
ES Electrophoresis Casting Tray, 7cm X 14cm |
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LE1011-A1 | GenDepot | Ea | EUR 198 |
ES Electrophoresis Casting Tray, 7cm X 7cm |
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LE1011-A2 | GenDepot | Ea | EUR 181.2 |
Electrophoresis Buffer (10×) |
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E-BC-R331-100mL | Elabscience Biotech | 100mL | EUR 15 |
Electrophoresis Buffer (10×) |
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E-BC-R331-200mL | Elabscience Biotech | 200mL | EUR 20 |
Electrophoresis Buffer (10×) |
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E-BC-R331-500mL | Elabscience Biotech | 500mL | EUR 25 |
Electrophoresis Buffer (10×) |
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E-BC-R331-each | Elabscience Biotech | each | Ask for price |
5 x SDS Electrophoresis Buffer |
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GR103039 | Genorise Scientific | 1 L | EUR 107 |
Agarose Gel Electrophoresis Kit |
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MB570-10PR | EWC Diagnostics | 1 unit | EUR 38.47 |
Description: Agarose Gel Electrophoresis Kit |
ES 8/10 Double Gel Comb For ES Electrophoresis |
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LE1011-A3 | GenDepot | Ea | EUR 148.8 |
4 x Phosphate/SDS Electrophoresis Buffer |
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GR103033 | Genorise Scientific | 1 L | EUR 116 |
electrophoresis ps 1500v, 300ma, 150w |
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EV2230 | Consort | ea | EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
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EV2230-UK | Consort | ea | EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
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EV2230-US | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
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EV2320 | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
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EV2320-UK | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
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EV2320-US | Consort | ea | EUR 898.8 |
electrophoresis ps 1200v, 500ma, 300w |
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EV3150 | Consort | ea | EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
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EV3150-UK | Consort | ea | EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
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EV3150-US | Consort | ea | EUR 960 |
electrophoresis ps 3000v, 300ma, 300w |
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EV3330 | Consort | ea | EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
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EV3330-UK | Consort | ea | EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
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EV3330-US | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
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EV3620 | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
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EV3620-UK | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
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EV3620-US | Consort | ea | EUR 1456.8 |
Electrophoresis Buffer (10x) |
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MBS2557002-100mL | MyBiosource | 100mL | EUR 85 |
Electrophoresis Buffer (10x) |
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MBS2557002-200mL | MyBiosource | 200mL | EUR 90 |
Electrophoresis Buffer (10x) |
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MBS2557002-500mL | MyBiosource | 500mL | EUR 95 |
Electrophoresis Buffer (10x) |
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MBS2557002-5x500mL | MyBiosource | 5x500mL | EUR 380 |
electrophoresis ps 400v, 500ma, 50w |
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EV1450 | Consort | ea | EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
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EV1450-UK | Consort | ea | EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
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EV1450-US | Consort | ea | EUR 574.8 |
electrophoresis ps 300v, 1000ma, 150w |
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EV2310 | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
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EV2310-UK | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
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EV2310-US | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EV2650 | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EV2650-UK | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EV2650-US | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 2000ma, 300w |
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EV3020 | Consort | ea | EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
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EV3020-UK | Consort | ea | EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
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EV3020-US | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
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EV3610 | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
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EV3610-UK | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
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EV3610-US | Consort | ea | EUR 960 |
TEMED, electrophoresis grade |
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RM10358-100G | EWC Diagnostics | 1 unit | EUR 301.18 |
Description: TEMED, electrophoresis grade |
Agarose Gel Electrophoresis Kit (Teaching) |
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66602 | Sisco Laboratories | 15 expt. Kit | EUR 44.05 |
Description: Part E |
Electrophoresis Power Supply |
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LA690-1NO | EWC Diagnostics | 1 unit | EUR 432.84 |
Description: Electrophoresis Power Supply |
electrophoresis power supply |
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EV245 | Consort | ea | EUR 574.8 |
ES 6 Tooth Gel Comb For ES Electrophoresis |
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LE1011-A4 | GenDepot | Ea | EUR 148.8 |
HiPer® Agarose Gel Electrophoresis Teach |
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HTBM001-10PR | EWC Diagnostics | 1 unit | EUR 29.56 |
Description: HiPer® Agarose Gel Electrophoresis Teach |
Electrophoresis Apparatus - 7x10cm (6-10-well) |
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EDK611 | Bio Basic | 1 UNIT | EUR 383.11 |
Electrophoresis Apparatus - 7x14cm (6-10-well) |
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EDK613 | Bio Basic | 1 UNIT | EUR 487.51 |
Glycine for electrophoresis, 99.5% |
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52574 | Sisco Laboratories | 100 Gms | EUR 9.58 |
Description: Part A |
Agarose for DNA electrophoresis |
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16-0027-500 | Medicago | 500 g | EUR 410.4 |
10X MOPS Electrophoresis Buffer |
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ML050-500ML | EWC Diagnostics | 1 unit | EUR 32.7 |
Description: 10X MOPS Electrophoresis Buffer |
CometAssay Electrophoresis System |
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4250-050-ES | Biotechne | 1 Kit | EUR 3540.96 |
myGel Mini Electrophoresis System |
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E1101 | Benchmark Scientific | 1 each | EUR 636.38 |
myGel Mini Electrophoresis System |
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BM0286 | GenDepot | System | EUR 741.6 |
SDS-PAGE Electrophoresis Buffer |
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MBS355536-1L | MyBiosource | 1L | EUR 165 |
SDS-PAGE Electrophoresis Buffer |
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MBS355536-5x1L | MyBiosource | 5x1L | EUR 480 |
SDS-PAGE Electrophoresis Buffer |
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MBS355542-10x1L | MyBiosource | 10x1L | EUR 250 |
6X6 well Electrophoresis Apparatus |
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EDK626 | Bio Basic | 1 UNIT | EUR 750.6 |
miniONE Electrophoresis System - EACH |
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ELE3400 | Scientific Laboratory Supplies | EACH | EUR 777.6 |
Starch (Smithies); for electrophoresis |
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GE6857 | Glentham Life Sciences | 100g | EUR 253.58 |
Starch (Smithies), for electrophoresis |
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GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 370.8 |
Starch (Smithies), for electrophoresis |
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GE6857-500G | Glentham Life Sciences | 500 g | EUR 217.2 |
CometAssay Single Cell Gel Electrophoresis Assay |
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4250-050-K | Biotechne | 50 tests | EUR 513.6 |
electrophoresis power supply 120 vac |
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EV245-US | Consort | ea | EUR 574.8 |
10-well protein Vertical Electrophoresis Apparatus |
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EDK692 | Bio Basic | 1 UNIT | EUR 487.51 |
Aminoacetic acid, ?For electrophoresis |
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GRM2233-100G | EWC Diagnostics | 1 unit | EUR 32.26 |
Description: Aminoacetic acid, ?For electrophoresis |
Aminoacetic acid, ?For electrophoresis |
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GRM2233-500G | EWC Diagnostics | 1 unit | EUR 144.96 |
Description: Aminoacetic acid, ?For electrophoresis |
electrophoresis power supply uk plug |
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EV245-UK | Consort | ea | EUR 574.8 |
WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell |
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HTES1013 | Shanghai WSHT Biotechnology | set | EUR 602 |
WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell |
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HTES1014 | Shanghai WSHT Biotechnology | set | EUR 448 |
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well) |
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EDK614 | Bio Basic | 1 UNIT | EUR 520.92 |
CometAssay Electrophoresis Starter Kit |
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4250-050-ESK | Biotechne | 1 Kit | EUR 3749.28 |
Gel Electrophoresis System with Time Control, 220-240 V |
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Q-EP-2114 | ABM | 1 | EUR 1180 |
Agarose, medium EEO ?For electrophoresis |
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RM6249-100G | EWC Diagnostics | 1 unit | EUR 119.06 |
Description: Agarose, medium EEO ?For electrophoresis |
Agarose, medium EEO ?For electrophoresis |
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RM6249-10G | EWC Diagnostics | 1 unit | EUR 19.63 |
Description: Agarose, medium EEO ?For electrophoresis |
Agarose, medium EEO ?For electrophoresis |
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RM6249-1KG | EWC Diagnostics | 1 unit | EUR 1089.43 |
Description: Agarose, medium EEO ?For electrophoresis |
Agarose, medium EEO ?For electrophoresis |
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RM6249-25G | EWC Diagnostics | 1 unit | EUR 36.87 |
Description: Agarose, medium EEO ?For electrophoresis |
Alkaline Hemoglobin Electrophoresis Kit |
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MBP001-112RUNS | EWC Diagnostics | 1 unit | EUR 118.87 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Hemoglobin Electrophoresis Kit |
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MBP001-224RUNS | EWC Diagnostics | 1 unit | EUR 214 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Hemoglobin Electrophoresis Kit |
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MBP008-224RUNS | EWC Diagnostics | 1 unit | EUR 208.42 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Acrylamide, suitable for electrophoresis |
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GK5651 | Glentham Life Sciences | 100g | EUR 21.8 |
Acrylamide, suitable for electrophoresis |
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GK5651-100 | Glentham Life Sciences | 100 | EUR 23.8 |
Acrylamide, suitable for electrophoresis |
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GK5651-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Alkaline Paper Electrophoresis Kit (Cell |
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MBP009-20RUNS | EWC Diagnostics | 1 unit | EUR 59.3 |
Description: Alkaline Paper Electrophoresis Kit (Cell |
WEE VERT PROTEIN ELECTROPHORESIS SYSTEM |
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LA1070-1NO | EWC Diagnostics | 1 unit | EUR 1258.71 |
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM |
Electrophoresis Power Supply (2 termina |
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LA842-1NO | EWC Diagnostics | 1 unit | EUR 66.05 |
Description: Electrophoresis Power Supply (2 termina |
Cellulose Acetate Electrophoresis System |
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LA1027-1NO | EWC Diagnostics | 1 unit | EUR 534.37 |
Description: Cellulose Acetate Electrophoresis System |
Chiller for Electrophoresis systems - EACH |
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CSLCHILLER | Scientific Laboratory Supplies | EACH | EUR 7642.35 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-1 | Glycomatrix | 15 mL | EUR 17.3 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-2 | Glycomatrix | 25 mL | EUR 23.23 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-3 | Glycomatrix | 50 mL | EUR 35.42 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-4 | Glycomatrix | 100 mL | EUR 62.99 |
WSHT-Basic Electrophoresis Power Supply |
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HTES1001 | Shanghai WSHT Biotechnology | set | EUR 557.2 |
Electrophoresis baffle for Life mini tank |
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HTDB03 | Shanghai WSHT Biotechnology | pcs | EUR 5.6 |
20X DoubleQuick DNA Electrophoresis Buffer |
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73530 | Sisco Laboratories | 250 ml | EUR 35.58 |
Description: Part E |
20X DoubleQuick DNA Electrophoresis Buffer |
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73530-1 | Sisco Laboratories | 500 ml | EUR 64.4 |
Description: Part E |
Gel Electrophoresis System with Muticaster, 100-120 V Input |
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Q-EP-2034 | ABM | 1 | EUR 1715 |
Gel Electrophoresis System with Muticaster, 220-240 V Input |
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Q-EP-2056 | ABM | 1 | EUR 2125 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699 | Glentham Life Sciences | 10g | EUR 463.47 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-10 | Glentham Life Sciences | 10 | EUR 28.4 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-100 | Glentham Life Sciences | 100 | EUR 142.4 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-25 | Glentham Life Sciences | 25 | EUR 50.6 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-250 | Glentham Life Sciences | 250 | EUR 284.8 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-500 | Glentham Life Sciences | 500 | EUR 506.2 |
Gel Electrophoresis System with Time Control, 100-120 V Input |
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Q-EP-2100 | ABM | 1 | EUR 1385 |
CometAssay Single Cell Gel Electrophoresis Assay-Silver |
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4251-050-K | Biotechne | 1 kit | EUR 684.96 |
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579833-005mL | MyBiosource | 0.05mL | EUR 195 |
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579833-01mL | MyBiosource | 0.1mL | EUR 255 |
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579833-5x005mL | MyBiosource | 5x0.05mL | EUR 820 |
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579833-5x01mL | MyBiosource | 5x0.1mL | EUR 1100 |
WSHT-Universal Electrophoresis Power Supply |
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HTES1003 | Shanghai WSHT Biotechnology | set | EUR 784 |
Ammonium Persulphate (APS) for electrophoresis, 99% |
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28575 | Sisco Laboratories | 25 Gms | EUR 1.51 |
Description: Part A |
Electrophoresis baffle for Life Technology Novex |
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HTDB02 | Shanghai WSHT Biotechnology | pcs | EUR 2.8 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
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20840057-1 | Glycomatrix | 500 mL | EUR 74.66 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
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20840057-2 | Glycomatrix | 1 L | EUR 139.51 |
WSHT-miniPRO4 Mini Vertical Electrophoresis Cell |
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HTES1009 | Shanghai WSHT Biotechnology | set | EUR 795.2 |
WSHT-miniPRO2 Mini Vertical Electrophoresis Cell |
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HTES1010 | Shanghai WSHT Biotechnology | set | EUR 516.6 |
WSHT-easyPRO4 Mini Vertical Electrophoresis Cell |
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HTES1011 | Shanghai WSHT Biotechnology | set | EUR 837.2 |
WSHT-easyPRO2 Mini Vertical Electrophoresis Cell |
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HTES1012 | Shanghai WSHT Biotechnology | set | EUR 544.6 |
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380 |
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ACT-CBMN-7 | ACTGene | each | EUR 892.8 |
Electrophoresis baffle for Bio-Rad Mini-PROTEAN |
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HTDB01 | Shanghai WSHT Biotechnology | pcs | EUR 2.1 |
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579830-005mL | MyBiosource | 0.05mL | EUR 180 |
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579830-01mL | MyBiosource | 0.1mL | EUR 230 |
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579830-05mL | MyBiosource | 0.5mL | EUR 695 |
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579830-5x005mL | MyBiosource | 5x0.05mL | EUR 760 |
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade) |
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MBS8579830-5x01mL | MyBiosource | 5x0.1mL | EUR 980 |
Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH |
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ELE1111 | Scientific Laboratory Supplies | EACH | EUR 961.2 |
WSHT-Multi-Functional Electrophoresis Power Supply |
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HTES1004 | Shanghai WSHT Biotechnology | set | EUR 896 |
WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell |
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HTES2001-P | Shanghai WSHT Biotechnology | set | EUR 459.2 |
myGel Mini Electrophoresis System 230V (includes transformer) |
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E1101-E | Benchmark Scientific | 1 PC | EUR 665.55 |
myGel Mini Electrophoresis System 230V (includes transformer) |
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BM0291 | GenDepot | System | EUR 789.6 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
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4250-050-ESK-PS2 | Biotechne | 1 Kit | EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
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4250-050-ESK-PS3 | Biotechne | 1 Kit | EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
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4250-050-ESK-PS4 | Biotechne | 1 Kit | EUR 4597.68 |
WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster) |
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HTES2001 | Shanghai WSHT Biotechnology | set | EUR 322 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
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E1WP3231 | EnoGene | 50T | EUR 100 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
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E1WP3232 | EnoGene | 100T | EUR 150 |
High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa) |
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MKPZ010-200mL | Shanghai WSHT Biotechnology | 200μL | EUR 72 |
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system) |
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E1WP326 | EnoGene | 50T | EUR 100 |
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system) |
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E1WP324 | EnoGene | 50T | EUR 60 |
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator |
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E1201 | Benchmark Scientific | 1 each | EUR 1001.7 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100) |
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E1101-SK | Benchmark Scientific | 1 each | EUR 727.06 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100) |
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E1101-SK-E | Benchmark Scientific | 1 PC | EUR 757.35 |
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input |
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E1201-E | Benchmark Scientific | 1 each | EUR 1058.9 |
OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH |
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VS10WCES | Scientific Laboratory Supplies | EACH | EUR 1614.21 |
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade) |
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E37F007 | EnoGene | 100-μL | EUR 226.67 |
Description: Reagents-for-ssDNA-or-RNA-electrophoresis |
SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade) |
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E37F001 | EnoGene | 100-μL | EUR 226.67 |
Description: Reagents-for-dsDNA-electrophoresis |
Axygen Horizontal Gel Box 20Cm - EACH |
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HGB-20 | Scientific Laboratory Supplies | EACH | EUR 1012.5 |
AXYGEN® HORIZONTAL GEL BOX, 10 CM |
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HGB-10 | CORNING | 1/pk | EUR 399.6 |
Description: Lab Equipment; Axygen Branded EQ |
Horizontal head, for 12 x 1.5ml |
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BCM1604 | Bio Basic | 1 pcs, 1 UNIT | EUR 200.68 |
Horizontal head, for 12 x 1.5ml |
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BV1000-H15 | Benchmark Scientific | 1 PC | EUR 111.76 |
Horizontal head, for 4 x 15ml |
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BCM1605 | Bio Basic | 1 pcs, 1 UNIT | EUR 200.68 |
Horizontal head, for 2 x 50ml |
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BCM1606 | Bio Basic | 1 pcs, 1 UNIT | EUR 200.68 |
Horizontal head, for 4 x 15ml |
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BV1000-H150 | Benchmark Scientific | 1 PC | EUR 111.76 |
Horizontal head, for 2 x 50ml |
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BV1000-H500 | Benchmark Scientific | 1 PC | EUR 111.76 |
Midi Horizontal Gel Unit |
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ELE8120 | Scientific Laboratory Supplies | EACH | EUR 630.42 |
Horizontal head for 12 x 1.5mL - EACH |
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MIX7018 | Scientific Laboratory Supplies | EACH | EUR 164.7 |
Horizontal head for 4 x 15mL - EACH |
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MIX7020 | Scientific Laboratory Supplies | EACH | EUR 164.7 |
Taken together, these findings indicate that our method allows semi-quantitative analysis to estimate the ratio of human antibodies compared with the IC included in the total antibody. This method was successfully applied to the in vivo studies using rats, and the data helps explain the unexpectedly rapid clearance of human antibodies for the formation of large IC. The combination of IC separation by BN-PAGE and detection of peptide-specific human IgG with LC-MS / MS bioanalytical approach is common, useful for detecting IC in animals.