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Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches.

Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches.
Posted by Troy

Medaka Sea is one of the most popular models of fish species for Ecotoxicology and environmental research and study of proteomics is a useful tool for understanding the medaka molecular response after exposure to different environmental stresses. High-quality protein sample preparation is the key to producing high-quality two-dimensional gel electrophoresis (2-DE) results for proteomics analysis.

In recent years, the extraction of protein-based Trizol has gained popularity because of its performance promise in producing high-quality 2-DE and comfort method.Three Trizol-based approach (Trizol method, the method and the method of Trizol Trizol aliquots with commercial cleaning kit) is used to extract protein from marine samples medaka and 2-DE profiles produced. 2-DE profile quality and effectiveness of extraction methods were evaluated.

For comparison, two common protein extraction method (method of lysis buffer and trichloroacetic acid (TCA) / acetone precipitation extraction) are also applied in parallel to Trizol based approaches.Any three Trizol-based approach produces high-quality 2-DE profiles of marine medaka compared with both methods of lysis buffer and TCA / acetone precipitation extraction.

In addition, the Trizol method with clean-up kit produced the best commercial 2-DE profile in terms of the clarity of the background, the number of spots and resolution proteins.Trizol based approach offered a better choice than traditional protein extraction method for 2-DE analysis of medaka sea. A modified version of the method Trizol with commercial clean-up kit was shown to produce the best 2-DE profile.

Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches.
Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches.

A method of combining blue native polyacrylamide gel electrophoresis liquid chromatography-tandem mass spectrometry for the detection of circulating immune complexes between the monoclonal antibody therapy and anti-drug antibodies in animals.

Monoclonal antibody therapy can potentially induce unwanted immune response, so that the production of anti-drug antibodies (ADAS). Binding of fennel drug and subsequent formation of immune complexes (ICs) can trigger a variety of responses, depending on the size, concentration, and a subclass of fennel. To better understand the impact of fennel in pharmacokinetics, pharmacodynamics and toxicology profile, bioanalytical methods developed to detect IC between a human monoclonal immunoglobulin G (IgG) and fennel in a biological sample.

Regarding the experimental procedure, in brief, human-specific antibodies and human antibodies bound IC in biological samples separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The target fraction is then cut from the gel, followed by in-gel trypsin digestion and subsequent liquid chromatography-tandem mass spectrometry (LC-MS / MS) to monitor the human peptide-specific IgG. This method is capable of detecting various types of human antibodies with a lower detection limit of 10 ug / mL in monkey serum. Performance IC assay to detect the expressed using spiked samples, and pre-incubated in monkey serum IC clearly detectable.

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1602

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis power supply

EV245 ea
EUR 574.8

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

miniONE Electrophoresis System

ELE3400 EACH
EUR 691.2

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

myGel Mini Electrophoresis System

E1101 1 each
EUR 623.9

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6793.2

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 854.4

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 712.8

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1434.85

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

Axygen Horizontal Gel Box 20Cm

HGB-20 EACH
EUR 900

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 12 x 1.5ml

BV1000-H15 1 PC
EUR 108.5

Horizontal head for 12 x 1.5mL

MIX7018 EACH
EUR 146.4

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 4 x 15ml

BV1000-H150 1 PC
EUR 108.5

Horizontal head, for 2 x 50ml

BV1000-H500 1 PC
EUR 108.5

Horizontal head for 4 x 15mL

MIX7020 EACH
EUR 146.4

Horizontal head for 2 x 50mL

MIX7022 EACH
EUR 146.4

Midi Horizontal Gel Unit

ELE8120 EACH
EUR 630.42

6 x 15mL horizontal rotisserie 1 ea

MIX7080 EACH
EUR 92.4

6 x 50mL horizontal rotisserie 1 ea

MIX7082 EACH
EUR 92.4

6 x 15ml horizontal rotisserie, 1 ea

R4040-HZ15 1 PC
EUR 68

6 x 50ml horizontal rotisserie, 1 ea

R4040-HZ50 1 PC
EUR 68

midiplus horizontal unit 15x7-10-15 cm

EHS1300-SYS ea
EUR 496.8

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 583.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 343.2
Description: Lab Equipment; Axygen Branded EQ

MAGic Clamp Tube Rack 4 x 15mL tubes horizontal

MIX7174 EACH
EUR 163.2

MAGic Clamp Tube Rack 2 x 50mL tubes horizontal

MIX7176 EACH
EUR 163.2

Pipette Tray PVC Horizontal

PIP5500 EACH
EUR 44.4

Horizontal 50mL Tube Holder

MIX5088 EACH
EUR 176.4

MAGic Clamp Tube Rack 12 x 1.5/2.0mL tubes horizontal

MIX7172 EACH
EUR 163.2

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 381.6

MAGic Clamp™ Tube Rack, 4 x 15ml tubes, horizontal

H1000-MR-T150H 1 PC
EUR 122

MAGic Clamp™ Tube Rack, 2 x 50ml tubes, horizontal

H1000-MR-T500H 1 PC
EUR 122

mini horizontal unit 7x10 cm

EHS1102-SYS ea
EUR 381.6

midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 422.4

mini horizontal unit 9x11 cm

EHS3200-SYS ea
EUR 595.2

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
EUR 930

wide horizontal unit 23.5x25 cm

EHS3610-SYS ea
EUR 1030.8

Horizontal orientator 384 wells

DD53423 PK2
EUR 78

midi horizontal unit 10x10 cm

EHS1202-SYS ea
EUR 422.4

maxi horizontal unit 20x10 cm

EHS1401-SYS ea
EUR 686.4

maxi horizontal unit 20x20 cm

EHS1402-SYS ea
EUR 727.2

maxi horizontal unit 20x25 cm

EHS1403-SYS ea
EUR 747.6

mini horizontal unit 12x14 cm

EHS3300-SYS ea
EUR 625.2

midi horizontal unit 15x15 cm

EHS3400-SYS ea
EUR 757.2

midi horizontal unit 15x25 cm

EHS3410-SYS ea
EUR 930

midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 980.4

MAGic Clamp™ Tube Rack, 12 x 1.5/2.0ml tubes, horizontal

H1000-MR-T15H 1 PC
EUR 122

HS 260 Basic Horizontal Shaker

3066602 EACH
EUR 3482.4

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 433.2

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
EUR 422.4

midiplus horizontal unit 15x10 cm

EHS1302-SYS ea
EUR 433.2

midiplus horizontal unit 15x15 cm

EHS1303-SYS ea
EUR 433.2

maxiplus horizontal unit 26x16 cm

EHS1501-SYS ea
EUR 939.6

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
EUR 939.6

maxiplus horizontal unit 26x32 cm

EHS1503-SYS ea
EUR 939.6

midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 453.6

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 727.2

Horizontal outer dims 415h x 140w x 560d with 11 apertures of approx 37mm

AH17911 EACH
EUR 184.8

Horizontal outer dims 415h x 140w x 560d with 6 apertures of approx 67mm

AH1796UT EACH
EUR 208.8

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS ea
EUR 1132.8

Horizontal Plastic Clip Microtube Holder

MIX5096 EACH
EUR 190.8

Tube Rack for 7x50ml QuEChERs, horizontal

BV1010-50HQ 1 PC
EUR 42.4

Tube Rack for 7x50mL QuEChERs horizontal

MIX7044 EACH
EUR 57.6

maxiplus horizontal unit 26x16-24-32cm

EHS1500-SYS ea
EUR 1132.8

Genie Horizontal Orbital Shaker Attachments

MIX5321 EACH
EUR 603.6

10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-01 Ea
EUR 153.6

10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-02 Ea
EUR 153.6

Casting Dams, For Enduro 10cm Gel System

LE1000CD-02 Ea
EUR 165.6

6 x 1.5/2.0ml tube holder, pack of 2 (Installed for vertical or horizontal mixing)

R4040-1520 1 PC
EUR 22.3

Horizontal shelf for freezer capacity for 16 boxes height 50 mm

DD37581 EACH
EUR 460.8

12 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-04 Ea
EUR 153.6

12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-05 Ea
EUR 153.6

16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-07 Ea
EUR 153.6

16 Well Comb ,2mm Thick For Enduro 10cm Gel System

LE1000C10-08 Ea
EUR 153.6

25 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-11 Ea
EUR 153.6

8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
EUR 153.6

12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-03 Ea
EUR 153.6

16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-06 Ea
EUR 153.6

Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

M8-L18SP-15H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

M8-L18SP-50H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-09 Ea
EUR 153.6

20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-10 Ea
EUR 153.6

5 gel caster for 8 x 10cm or 10 x 10cm gels.

CSL6CAST EACH
EUR 582.3

Horizontal MicroLoops E Assortment - 10 each of 15x150 and 50x500 micron aperture mounts; mounted on 18 mm rods

M8-L18SP-HA1 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E Assortment - 10 each of 15x150 and 50x500 micron aperture mounts; mounted on 18 mm rods

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

B-M8-HA1-B1 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

B-M8-HA1-B1A 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

B-M8-HA1-B3 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

B-M8-HA1-B3S 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

B-M8-HA1-B5 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
EUR 157.2

10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-02 Ea
EUR 157.2

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

B-M8-15H-B1 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

B-M8-15H-B1A 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

B-M8-15H-B3 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

B-M8-15H-B3S 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

B-M8-15H-B5 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

B-M8-50H-B1 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

B-M8-50H-B1A 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

B-M8-50H-B3 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

B-M8-50H-B3S 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

B-M8-50H-B5 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

Taken together, these findings indicate that our method allows semi-quantitative analysis to estimate the ratio of human antibodies compared with the IC included in the total antibody. This method was successfully applied to the in vivo studies using rats, and the data helps explain the unexpectedly rapid clearance of human antibodies for the formation of large IC. The combination of IC separation by BN-PAGE and detection of peptide-specific human IgG with LC-MS / MS bioanalytical approach is common, useful for detecting IC in animals.

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