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Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Posted by Troy

glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous.

Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach. Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges.

Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of ​​acid gel. This study provides an effective approach for separating isomer weight protein glycosylation drug charges, and quantitative exploring the site-specific N-glycans dynamics along with the different isomers charge.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

PCR-Restriction Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Characterization of Listeria monocytogenes isolates of Ready-to-Eat Foods, Food Processing Environmental and Clinical Samples in South Africa.

Listeria monocytogenes is a ubiquitous, intracellular foodborne pathogen responsible for invasive listeriosis. The ability of L. monocytogenes causes the disease has some correlation with serotype specific group of offspring, making identification of the lineage is important for epidemiological analysis. Development of methods for connecting strain typing for outbreak of listeriosis L. monocytogenes will help minimize the spread of disease. The purpose of this study was to design a PCR-restriction fragment length polymorphism (RFLP) method for distinguishing between groups of lineages of L. monocytogenes.

PCR-amplified gene fragment hly for 12 serotypes of L. monocytogenes were sequenced, aligned, and analyzed by Bioedit program, and single nucleotide polymorphisms (SNPs) in the gene region identified. Because of the difficulty in obtaining serotype reference strain 4AB, these serotypes not included in this study. We tested the specificity and accuracy of PCR-RFLP method in the reference strains of L. monocytogenes and validated the method with 172 L. monocytogenes strains recovered from humans, food, environmental and food processing in 2000 to 2002 and from 2008 to 2010 from regions in Africa South.

PCR-RFLP analysis applied in this study L. monocytogenes serotype were placed into one of three groups based on different lineages and SNP sequences each lineage group. SNP preserved in areas where RFLP analysis can be applied to the difference between L. monocytogenes groups.This genealogical studies were conducted to evaluate the implementation of the original agarose gel electrophoresis reported previously for analysis of various monoclonal and polyclonal antibodies.

Agarose Gel Electrophoresis Kit

MB570-10PR 1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit

HiPer® Agarose Gel Electrophoresis Teach

HTBM001-10PR 1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 17.3

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 23.23

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 35.42

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 62.99

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell

HTES1013 set
EUR 602

WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell

HTES1014 set
EUR 448

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Aminoacetic acid, ?For electrophoresis

GRM2233-100G 1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis

Aminoacetic acid, ?For electrophoresis

GRM2233-500G 1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Chiller for Electrophoresis systems - EACH

CSLCHILLER EACH
EUR 7642.35

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

Electrophoresis baffle for Life mini tank

HTDB03 pcs
EUR 5.6

High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa)

MKPZ010-200mL 200μL
EUR 72

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

Electrophoresis Buffer (10×)

E-BC-R331-100mL 100mL
EUR 15

Electrophoresis Buffer (10×)

E-BC-R331-200mL 200mL
EUR 20

Electrophoresis Buffer (10×)

E-BC-R331-500mL 500mL
EUR 25

Electrophoresis Buffer (10×)

E-BC-R331-each each Ask for price

Electrophoresis baffle for Life Technology Novex

HTDB02 pcs
EUR 2.8

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 74.66

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 139.51

TEMED, electrophoresis grade

RM10358-100G 1 unit
EUR 301.18
Description: TEMED, electrophoresis grade

electrophoresis power supply

EV245 ea
EUR 574.8

Electrophoresis Power Supply

LA690-1NO 1 unit
EUR 432.84
Description: Electrophoresis Power Supply

Electrophoresis baffle for Bio-Rad Mini-PROTEAN

HTDB01 pcs
EUR 2.1

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

10X MOPS Electrophoresis Buffer

ML050-500ML 1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster)

HTES2001 set
EUR 322

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

E1101 1 each
EUR 636.38

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

miniONE Electrophoresis System - EACH

ELE3400 EACH
EUR 777.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 651.6

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

Alkaline Hemoglobin Electrophoresis Kit

MBP001-112RUNS 1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP001-224RUNS 1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP008-224RUNS 1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Paper Electrophoresis Kit (Cell

MBP009-20RUNS 1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell

WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

LA1070-1NO 1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

Electrophoresis Power Supply (2 termina

LA842-1NO 1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina

Cellulose Acetate Electrophoresis System

LA1027-1NO 1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System

WSHT-Basic Electrophoresis Power Supply

HTES1001 set
EUR 557.2

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Hi-Eco Mini Horizontal Electrophoresis K

LA851-1NO 1 unit
EUR 205.56
Description: Hi-Eco Mini Horizontal Electrophoresis K

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

WSHT-Universal Electrophoresis Power Supply

HTES1003 set
EUR 784

ComboMiniTM Horizontal Electrophoresis System (110V)

CBMN-7 each
EUR 500

ComboMiniTM Horizontal Electrophoresis System (220V)

CBMN-8 each
EUR 530

WSHT-miniPRO4 Mini Vertical Electrophoresis Cell

HTES1009 set
EUR 795.2

WSHT-miniPRO2 Mini Vertical Electrophoresis Cell

HTES1010 set
EUR 516.6

WSHT-easyPRO4 Mini Vertical Electrophoresis Cell

HTES1011 set
EUR 837.2

WSHT-easyPRO2 Mini Vertical Electrophoresis Cell

HTES1012 set
EUR 544.6

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

WSHT-miniDNA Mini Horizontal Electrophoresis Cell

HTES1008 set
EUR 193.2

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

Agar, High Gel Strength

40100064-1 500 g
EUR 84.23

Agar, High Gel Strength

40100064-2 1 kg
EUR 135.61

Agar, High Gel Strength

40100064-3 2 kg
EUR 256.75

Agar, High Gel Strength

40100064-4 5 kg
EUR 557.75

Agar, high gel strength

GK9085-1 1
EUR 142.4

Agar, high gel strength

GK9085-100 100
EUR 23.8

Agar, high gel strength

GK9085-100G 100 g
EUR 64.8

Agar, high gel strength

GK9085-1KG 1 kg
EUR 208.8

Agar, high gel strength

GK9085-250 250
EUR 47.4

Agar, high gel strength

GK9085-250G 250 g
EUR 93.6

Agar, high gel strength

GK9085-500 500
EUR 81.5

Agar, high gel strength

GK9085-500G 500 g
EUR 134.4

Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH

ELE1111 EACH
EUR 961.2

WSHT-Multi-Functional Electrophoresis Power Supply

HTES1004 set
EUR 896

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell

HTES2001-P set
EUR 459.2

WSHT-liteDNA Universal Horizontal Electrophoresis Cell

HTES1007 set
EUR 259

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 665.55

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH

ELE2300 EACH
EUR 1802.25

WSHT-midiDNA Multipurpose Horizontal Electrophoresis Cell

HTES1006 set
EUR 278.6

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH

ELE2302 EACH
EUR 1891.35

Agar, low gel strength

GK2227-1 1
EUR 110.7

Agar, low gel strength

GK2227-100 100
EUR 19.8

Agar, low gel strength

GK2227-100G 100 g
EUR 60

Agar, low gel strength

GK2227-1KG 1 kg
EUR 170.4

Agar, low gel strength

GK2227-250 250
EUR 35.5

Agar, low gel strength

GK2227-250G 250 g
EUR 79.2

Agar, low gel strength

GK2227-500 500
EUR 63.3

Agar, low gel strength

GK2227-500G 500 g
EUR 112.8

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 727.06

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 757.35

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH

VS10WCES EACH
EUR 1614.21

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

Agar, low gel strength, suitable for microbiology

GC6234-1 1
EUR 122.6

Agar, low gel strength, suitable for microbiology

GC6234-100 100
EUR 23.8

Agar, low gel strength, suitable for microbiology

GC6234-100G 100 g
EUR 64.8

Agar, low gel strength, suitable for microbiology

GC6234-1KG 1 kg
EUR 184.8

Agar, low gel strength, suitable for microbiology

GC6234-250 250
EUR 39.4

Agar, low gel strength, suitable for microbiology

GC6234-250G 250 g
EUR 84

Agar, low gel strength, suitable for microbiology

GC6234-500 500
EUR 68.9

Agar, low gel strength, suitable for microbiology

GC6234-500G 500 g
EUR 118.8

Electrode Filling Sol Low Ionic Strength Applications - 100ML

EFSLIAPP 100ML
EUR 114.57

Agarose, High EEO

40100148-1 25 g
EUR 51.77

Agarose, High EEO

40100148-2 100 g
EUR 143.93

Agarose, high EEO

GE7747-1 1
EUR 660.6

Agarose, high EEO

GE7747-250 250
EUR 227.8

Agarose, high EEO

RM187-100G 1 unit
EUR 174.08
Description: Agarose, high EEO

Agarose, high EEO

RM187-10G 1 unit
EUR 18.22
Description: Agarose, high EEO

Agarose, high EEO

RM187-500G 1 unit
EUR 748.81
Description: Agarose, high EEO

Agarose High EEO

abx299903-100g 100 µg
EUR 387.5

Agarose High EEO

abx299903-20g 20 µg
EUR 275

Agarose High EEO

abx299903-50g 50 µg
EUR 325

Agarose, Low EEO

40100152-1 50 g
EUR 75.92

Agarose, Low EEO

40100152-2 100 g
EUR 140.08

Agarose, Low EEO

40100152-3 500 g
EUR 446.01

Agarose, low EEO

GE0001-1 1
EUR 569.5

Agarose, low EEO

GE0001-100 100
EUR 102.7

Agarose, low EEO

GE0001-100G 100 g
EUR 160.8

Agarose, low EEO

GE0001-1KG 1 kg
EUR 724.8

Agarose, low EEO

GE0001-25 25
EUR 39.4

Agarose, low EEO

GE0001-250 250
EUR 189.8

Agarose, low EEO

GE0001-250G 250 g
EUR 265.2

Agarose, low EEO

GE0001-25G 25 g
EUR 84

Agarose, low EEO

GE0001-5 5
EUR 19

Agarose, low EEO

GE0001-500 500
EUR 316.4

Agarose, low EEO

GE0001-500G 500 g
EUR 418.8

Agarose, low EEO

GE0001-5G 5 g
EUR 58.8

Agarose Low EEO

abx299901-100g 100 µg
EUR 425

Agarose Low EEO

abx299901-20g 20 µg
EUR 300

Agarose Low EEO

abx299901-50g 50 µg
EUR 350

Agarose special, High EEO

MB176-100G 1 unit
EUR 143.94
Description: Agarose special, High EEO

Experiments were conducted to test the electrophoresis system for the characterization of different monoclonal antibodies and serum of animals, analysis of antibodies expressed in cell culture and antibody stability evaluation.

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