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Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Posted by Troy

glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous.

Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach. Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges.

Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of ​​acid gel. This study provides an effective approach for separating isomer weight protein glycosylation drug charges, and quantitative exploring the site-specific N-glycans dynamics along with the different isomers charge.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

PCR-Restriction Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Characterization of Listeria monocytogenes isolates of Ready-to-Eat Foods, Food Processing Environmental and Clinical Samples in South Africa.

Listeria monocytogenes is a ubiquitous, intracellular foodborne pathogen responsible for invasive listeriosis. The ability of L. monocytogenes causes the disease has some correlation with serotype specific group of offspring, making identification of the lineage is important for epidemiological analysis. Development of methods for connecting strain typing for outbreak of listeriosis L. monocytogenes will help minimize the spread of disease. The purpose of this study was to design a PCR-restriction fragment length polymorphism (RFLP) method for distinguishing between groups of lineages of L. monocytogenes.

PCR-amplified gene fragment hly for 12 serotypes of L. monocytogenes were sequenced, aligned, and analyzed by Bioedit program, and single nucleotide polymorphisms (SNPs) in the gene region identified. Because of the difficulty in obtaining serotype reference strain 4AB, these serotypes not included in this study. We tested the specificity and accuracy of PCR-RFLP method in the reference strains of L. monocytogenes and validated the method with 172 L. monocytogenes strains recovered from humans, food, environmental and food processing in 2000 to 2002 and from 2008 to 2010 from regions in Africa South.

PCR-RFLP analysis applied in this study L. monocytogenes serotype were placed into one of three groups based on different lineages and SNP sequences each lineage group. SNP preserved in areas where RFLP analysis can be applied to the difference between L. monocytogenes groups.This genealogical studies were conducted to evaluate the implementation of the original agarose gel electrophoresis reported previously for analysis of various monoclonal and polyclonal antibodies.

Agarose, low EEO

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EUR 265.2

Agarose, low EEO

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Agarose, low EEO

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EUR 418.8

Agarose, low EEO

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Agarose LE Low EEO

AXY1002 PK10
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EUR 199.2

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AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 201.6
Description: Bioscience Mol Bio; Agarose

Agar, low gel strength

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Agar, low gel strength

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Agar, high gel strength

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Agar, high gel strength

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Agar, low gel strength, suitable for microbiology

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Agar, low gel strength, suitable for microbiology

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Agar, low gel strength, suitable for microbiology

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Agarose, medium EEO

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HyAgarose? 3:1 Agarose, 100g

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AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 926.4
Description: Bioscience Mol Bio; Agarose

HyAgarose? HR Agarose, PCR Grade, 100g

R9012HR-100g each
EUR 398.4

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R9012LE-100g each
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Agarose

20-abx098147
  • EUR 260.40
  • EUR 126.00
  • 100 g
  • 1 g

Agarose

20-abx186369
  • EUR 610.80
  • EUR 360.00
  • 100 g
  • 25 g

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
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OdorEroder - 100g

ODER-100G 100ML
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HEPPS, 100g

R1605-100g each
EUR 228

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3669.6
Description: Bioscience Mol Bio; Agarose

AGAR, HIGH GEL

A01-102IHG-10kg 10 kg
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AGAR, HIGH GEL

A01-102IHG-2Kg 2 Kg
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A01-102IHG-500g 500 g
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Hi-Bind? T-Gel-Agarose

M1313-1 each
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M1313-10 each
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M1313-100 each
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Agarose

abx082006-100g 100 g
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Agarose

abx082007-100g 100 g
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Agarose

abx082008-100g 100 g
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Kimas high strength centrifuge tubes

Z514861-6EA PK6
EUR 93.02

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Gentamycin Sulfate, 100g

R1405-100g each
EUR 649.2

Kanamycin Sulfate, 100g

R2538-100g each
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Carbenicillin Disodium, 100g

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A0223-002 25g
EUR 343.2

HiQTM Low Melting Agarose

A0223-010 100g
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Agarose LM Low Melting

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Agarose LM Low Melting

AXY1006 PK10
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Agarose Gel DNA Extraction and Purification

EP10010 100 Tests
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Agarose StandardRoomTemperature

FYA002-100G 100 g Ask for price

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

Agarose GPG/LMP low melt

A2501-100000 100 g
EUR 510
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Agarose GPG/LMP low melt

A2501-25000 25 g
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Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

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110100-CG 5 ml
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110200-NG 5 ml
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EC11-G 1 ml
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KLH11-G 1 ml
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R9012LM-10g each
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R9012LM-500g each
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R9012LM-50g each
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20-103 25g/Unit
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20-104 100g/Unit
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Apex Low Melt Sieve Agarose Ultra Pure

20-245 100g/Unit
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KLH11-G-5 5 ml
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ToxOut™ High Capacity Endotoxin Removal Agarose

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Kimax Reuseable Glass Centrifuge tubes plain high strength 15ml

TUB2718 PK6
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dam for 15cm gel

EHS3400-DAM ea
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dam for 20cm gel

EHS3500-DAM ea
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dam for 23.5cm gel

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Protein A-Agarose High-Binding 6% Highly Crosslinked

P9301-050 50ml
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Protein A-Agarose High-Binding 6% Highly Crosslinked

P9301-100 100ml
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NAT1014 100G
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100G Tricine

NAT1054 100G
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NAT1090 100G
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NAT1500 100G
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NAT1526 100G
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CHR5468 EACH
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4250-050-ES 1 Kit
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miniONE Electrophoresis System

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G2008-500 5Kg
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GC0004-100G 100 g
EUR 54

Pectin, amidated, low ester

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Chitosan, low molecular weight

GC9009-100G 100 g
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Accuris Mini 100g Balance 100g/0.01g Readability

BAL1645 EACH
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Low Level TISAB II Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes

IS-BUF3-500 500ML
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Gel tray V (for Visirays)

2195934 2unit
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Description: Opt i on

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2394140 10unit
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gel casting base for evs3100

EVS3100-BASE ea
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gel casting base for evs3200

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Hydroxyethyl cellulose, high viscosity

GC2093-100G 100 g
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AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

Agarose

G060-1 100 g
EUR 211.2

Agarose

G060-2 500 g
EUR 442.8

Agarose

Z5050007 500 g
EUR 738
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Agarose

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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g

41028-100G 100G
EUR 266.4
Description: Minimum order quantity: 1 unit of 100G

pH/ATC Triode with epoxy body low maintenance gel

9107BNMD EACH
EUR 265.62

Combination pH electrode with epoxy body low maintenance gel

9146BN EACH
EUR 101.46

Carboxymethylcellulose sodium salt, low viscosity

GC7604-100G 100 g
EUR 49.2

Hydroxyethyl cellulose, very low viscosity

GC9711-100G 100 g
EUR 52.8

Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-050 500g
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Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-100 1Kg
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Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-500 5Kg
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Carboxymethylcellulose sodium salt, high viscosity

GC7698-100G 100 g
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100g HEPES Powder

61-034-RM EACH
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Calibration Weight 100g

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100G AquaPor ES

NAT1028 100G
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100G AquaPor LM

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100G SDS Ultrapure

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NAT1512 100G
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100G p-Terphenyl

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100G Bis-MSB

NAT1530 100G
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Flash NH2 100g

CHR5470 EACH
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C18(EC) 100g

CHR5488 EACH
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Flash C18 100g

CHR5490 EACH
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SCX-2 100g

CHR5874 EACH
EUR 607.62

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 222

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
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Gel-Bright LED Gel Illuminator

E90003 1EA
EUR 927.6
Description: Minimum order quantity: 1 unit of 1EA

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
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ES Horizontal Electrophoresis Set

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EUR 578.4

myGel Mini Electrophoresis System

E1101 1 each
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Gel Casting S for WSE-1710

2322107 4unit
EUR 339.6
Description: Gel Tr ay (S) x4, Gel Cast i ng St and (S), S si zecomb (9wel l /5wel l ) x2

Gel Casting L for WSE-1710

2322108 4unit
EUR 339.6
Description: Gel Tr ay (L ) x2, Gel Cast i ng St and (L ), S si zecomb (22wel l /12wel l ) x2

Gel Casting set for 10.5x6cm gels

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Gel Casting Stand for 5x6cm gels

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supporting mask for midi gel dryer

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ssilicone rubber for midi gel dryer

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supporting mask for maxi gel dryer

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silicone rubber for maxi gel dryer

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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

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Precast Agarose Gel TAE, 2%, 12 Well, EtBr Stained, 20 gels per Box

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TAE17-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TBE, 2%, 48 Well, EtBr Stained, 10 gels per Box

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Agarose: high melting temperature/medium resolution for all of your standard DNA applications 500 g

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Agarose, Sepro

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20-abx082386
  • EUR 326.40
  • EUR 260.40
  • 10 g
  • 5 g

Agarose M

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Agarose LE

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Agarose LE

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Agarose HR

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Agarose HR

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Agarose GA

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Agarose LM

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Description: Ultrapure agarose for separation and further analysis of PCR products.

Agarose A

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FYA002-500G 500 g Ask for price

Chitosan (5 cps), ultra low molecular weight

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Chitosan (10 cps), very low molecular weight

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Chitosan (100 - 300 cps), low molecular weight

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Chitosan (30 - 100 cps), low molecular weight

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Gel-FAST? Gel Staining/Destaining Kit

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Chitosan (1000 - 2000 cps), high molecular weight

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Gel Tray

2394250 4unit
EUR 324
Description: I ncl udi ng pl at es

Gel Tray

2398194 2unit
EUR 424.8

Experiments were conducted to test the electrophoresis system for the characterization of different monoclonal antibodies and serum of animals, analysis of antibodies expressed in cell culture and antibody stability evaluation.

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