glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous.
Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach. Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges.
Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of acid gel. This study provides an effective approach for separating isomer weight protein glycosylation drug charges, and quantitative exploring the site-specific N-glycans dynamics along with the different isomers charge.
Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
PCR-Restriction Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Characterization of Listeria monocytogenes isolates of Ready-to-Eat Foods, Food Processing Environmental and Clinical Samples in South Africa.
Listeria monocytogenes is a ubiquitous, intracellular foodborne pathogen responsible for invasive listeriosis. The ability of L. monocytogenes causes the disease has some correlation with serotype specific group of offspring, making identification of the lineage is important for epidemiological analysis. Development of methods for connecting strain typing for outbreak of listeriosis L. monocytogenes will help minimize the spread of disease. The purpose of this study was to design a PCR-restriction fragment length polymorphism (RFLP) method for distinguishing between groups of lineages of L. monocytogenes.
PCR-amplified gene fragment hly for 12 serotypes of L. monocytogenes were sequenced, aligned, and analyzed by Bioedit program, and single nucleotide polymorphisms (SNPs) in the gene region identified. Because of the difficulty in obtaining serotype reference strain 4AB, these serotypes not included in this study. We tested the specificity and accuracy of PCR-RFLP method in the reference strains of L. monocytogenes and validated the method with 172 L. monocytogenes strains recovered from humans, food, environmental and food processing in 2000 to 2002 and from 2008 to 2010 from regions in Africa South.
PCR-RFLP analysis applied in this study L. monocytogenes serotype were placed into one of three groups based on different lineages and SNP sequences each lineage group. SNP preserved in areas where RFLP analysis can be applied to the difference between L. monocytogenes groups.This genealogical studies were conducted to evaluate the implementation of the original agarose gel electrophoresis reported previously for analysis of various monoclonal and polyclonal antibodies.
Agarose Gel Electrophoresis Kit
MB570-10PR
EWC Diagnostics
1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit
Agarose Gel Electrophoresis Kit (Teaching)
66602
Sisco Laboratories
15 expt. Kit
EUR 44.05
Description: Part E
HiPer® Agarose Gel Electrophoresis Teach
HTBM001-10PR
EWC Diagnostics
1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach
Agarose for DNA electrophoresis
16-0027-500
Medicago
500 g
EUR 410.4
Agarose High Gel Strength
MBS635297-100g
MyBiosource
100g
EUR 215
Agarose High Gel Strength
MBS635297-1kg
MyBiosource
1kg
EUR 885
Agarose High Gel Strength
MBS635297-250g
MyBiosource
250g
EUR 340
Agarose High Gel Strength
MBS635297-500g
MyBiosource
500g
EUR 515
Agarose High Gel Strength
MBS635297-5x1kg
MyBiosource
5x1kg
EUR 3875
ES 6 Tooth Gel Comb For ES Electrophoresis
LE1011-A4
GenDepot
Ea
EUR 70
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-1
Bio-WORLD
15 mL
EUR 21.21
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-2
Bio-WORLD
25 mL
EUR 28.49
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-3
Bio-WORLD
50 mL
EUR 43.44
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-4
Bio-WORLD
100 mL
EUR 77.25
ES 8/10 Double Gel Comb For ES Electrophoresis
LE1011-A3
GenDepot
Ea
EUR 70
CometAssay Single Cell Gel Electrophoresis Assay
4250-050-K
Biotechne
50 tests
EUR 513.6
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3231
EnoGene
50T
EUR 100
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3232
EnoGene
100T
EUR 150
Glycine for electrophoresis, 99.5%
52574
Sisco Laboratories
100 Gms
EUR 9.58
Description: Part A
Electrophoresis Module for Eco-Mini, for 2 gels
AJ846-017-175
Westburg
each
EUR 604.95
Electrophoresis Module for Eco-Maxi, for 2 gels
AJ846-017-475
Westburg
each
EUR 997.35
Gel Electrophoresis System with Time Control, 220-240 V
Q-EP-2114
ABM
1
EUR 1180
CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K
Biotechne
1 kit
EUR 684.96
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)
E1WP324
EnoGene
50T
EUR 60
Aminoacetic acid, ?For electrophoresis
GRM2233-100G
EWC Diagnostics
1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis
Aminoacetic acid, ?For electrophoresis
GRM2233-500G
EWC Diagnostics
1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis
Gel Electrophoresis System with Muticaster, 100-120 V Input
Q-EP-2034
ABM
1
EUR 1715
Gel Electrophoresis System with Muticaster, 220-240 V Input
Q-EP-2056
ABM
1
EUR 2125
Gel Electrophoresis System with Time Control, 100-120 V Input
Q-EP-2100
ABM
1
EUR 1385
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)
E1WP326
EnoGene
50T
EUR 100
Ammonium Persulphate (APS) for electrophoresis, 99%
28575
Sisco Laboratories
25 Gms
EUR 1.51
Description: Part A
Tris-HEPES-SDS Buffer 20X for Electrophoresis
20840057-1
Bio-WORLD
500 mL
EUR 91.56
Tris-HEPES-SDS Buffer 20X for Electrophoresis
20840057-2
Bio-WORLD
1 L
EUR 173.27
electrophoresis ps 1500v, 300ma, 150w
EV2230
Consort
ea
EUR 898.8
electrophoresis ps 1500v, 300ma, 150w
EV2230-UK
Consort
ea
EUR 898.8
electrophoresis ps 1500v, 300ma, 150w
EV2230-US
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320-UK
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320-US
Consort
ea
EUR 898.8
ELECTROPHORESIS PS 1200V, 500MA, 300W
EV3150
Consort
ea
EUR 1060
ELECTROPHORESIS PS 1200V, 500MA, 300W
EV3150-UK
Consort
ea
EUR 1060
ELECTROPHORESIS PS 3000V, 200MA, 300W
EV3330
Consort
ea
EUR 1600
ELECTROPHORESIS PS 3000V, 200MA, 300W
EV3330-UK
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W
EV3620
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W
EV3620-UK
Consort
ea
EUR 1600
Electrophoresis Buffer (10x)
MBS2557002-100mL
MyBiosource
100mL
EUR 85
Electrophoresis Buffer (10x)
MBS2557002-200mL
MyBiosource
200mL
EUR 90
Electrophoresis Buffer (10x)
MBS2557002-500mL
MyBiosource
500mL
EUR 95
Electrophoresis Buffer (10x)
MBS2557002-5x500mL
MyBiosource
5x500mL
EUR 380
electrophoresis ps 400v, 500ma, 50w
EV1450
Consort
ea
EUR 574.8
electrophoresis ps 400v, 500ma, 50w
EV1450-UK
Consort
ea
EUR 574.8
electrophoresis ps 400v, 500ma, 50w
EV1450-US
Consort
ea
EUR 574.8
ELECTROPHORESIS PS 300V, 1000MA, 150W
EV2310
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 1000MA, 150W
EV2310-UK
Consort
ea
EUR 750
ELECTROPHORESIS PS 600V, 500MA, 150W
EV2650
Consort
ea
EUR 750
ELECTROPHORESIS PS 600V, 500MA, 150W
EV2650-UK
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 2000MA, 300W
EV3020
Consort
ea
EUR 1060
ELECTROPHORESIS PS 300V, 2000MA, 300W
EV3020-UK
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W
EV3610
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W
EV3610-UK
Consort
ea
EUR 1060
TEMED, electrophoresis grade
RM10358-100G
EWC Diagnostics
1 unit
EUR 301.18
Description: TEMED, electrophoresis grade
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380
ACT-CBMN-7
ACTGene
each
EUR 892.8
electrophoresis power supply
EV245
Consort
ea
EUR 574.8
Electrophoresis Power Supply
LA690-1NO
EWC Diagnostics
1 unit
EUR 432.84
Description: Electrophoresis Power Supply
SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F007-100-uL
EnoGene
100 μL
EUR 227
Description: Reagents for ssDNA or RNA electrophoresis
SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F007-50-uL
EnoGene
50 μL
EUR 133
Description: Reagents for ssDNA or RNA electrophoresis
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-005mL
MyBiosource
0.05mL
EUR 195
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-01mL
MyBiosource
0.1mL
EUR 255
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-5x005mL
MyBiosource
5x0.05mL
EUR 820
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-5x01mL
MyBiosource
5x0.1mL
EUR 1100
ELECTROPHORESIS PS 1200V, 500MA, 300W 120 VAC
EV3150-US
Consort
ea
EUR 1060
ELECTROPHORESIS PS 3000V, 200MA, 300W 120 VAC
EV3330-US
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W 120 VAC
EV3620-US
Consort
ea
EUR 1600
10X MOPS Electrophoresis Buffer
ML050-500ML
EWC Diagnostics
1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer
ELECTROPHORESIS PS 600V, 500MA, 150W 120VAC
EV2650-US
Consort
ea
EUR 750
CometAssay Electrophoresis System
4250-050-ES
Biotechne
1 Kit
EUR 3540.96
ELECTROPHORESIS PS 300V, 1000MA, 150W 120 VAC
EV2310-US
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 2000MA, 300W 120 VAC
EV3020-US
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W 120 VAC
EV3610-US
Consort
ea
EUR 1060
ES Horizontal Electrophoresis Set
LE1011
GenDepot
Set
EUR 410
SDS-PAGE Electrophoresis Buffer
MBS355536-1L
MyBiosource
1L
EUR 165
SDS-PAGE Electrophoresis Buffer
MBS355536-5x1L
MyBiosource
5x1L
EUR 480
SDS-PAGE Electrophoresis Buffer
MBS355542-10x1L
MyBiosource
10x1L
EUR 250
6X6 well Electrophoresis Apparatus
EDK626
Bio Basic
1 UNIT
EUR 750.6
electrophoresis power supply 120 vac
EV245-US
Consort
ea
EUR 574.8
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-100-uL
EnoGene
100 μL
EUR 227
Description: Reagents for dsDNA electrophoresis
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-500-uL
EnoGene
500 μL
EUR 983
Description: Reagents for dsDNA electrophoresis
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-50uL
EnoGene
50 μL
EUR 133
Description: Reagents for dsDNA electrophoresis
electrophoresis power supply uk plug
EV245-UK
Consort
ea
EUR 574.8
ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels
LE1001
GenDepot
Set
EUR 450
myGel Mini Electrophoresis System, 115V
BM0286
GenDepot
System
EUR 560
CometAssay Electrophoresis Starter Kit
4250-050-ESK
Biotechne
1 Kit
EUR 3749.28
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-005mL
MyBiosource
0.05mL
EUR 180
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-01mL
MyBiosource
0.1mL
EUR 230
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-05mL
MyBiosource
0.5mL
EUR 695
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-5x005mL
MyBiosource
5x0.05mL
EUR 760
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-5x01mL
MyBiosource
5x0.1mL
EUR 980
ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels
LE1002
GenDepot
Set
EUR 470
ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels
LE1003
GenDepot
Set
EUR 410
ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels
LE1004
GenDepot
Set
EUR 610
ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels
LE1005
GenDepot
Set
EUR 710
ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels
LE1006
GenDepot
Set
EUR 750
Alkaline Hemoglobin Electrophoresis Kit
MBP001-112RUNS
EWC Diagnostics
1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Hemoglobin Electrophoresis Kit
MBP001-224RUNS
EWC Diagnostics
1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Hemoglobin Electrophoresis Kit
MBP008-224RUNS
EWC Diagnostics
1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Paper Electrophoresis Kit (Cell
MBP009-20RUNS
EWC Diagnostics
1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell
WEE VERT PROTEIN ELECTROPHORESIS SYSTEM
LA1070-1NO
EWC Diagnostics
1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM
Electrophoresis Power Supply (2 termina
LA842-1NO
EWC Diagnostics
1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina
Cellulose Acetate Electrophoresis System
LA1027-1NO
EWC Diagnostics
1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System
ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2
GenDepot
Ea
EUR 90
20X DoubleQuick DNA Electrophoresis Buffer
73530
Sisco Laboratories
250 ml
EUR 35.58
Description: Part E
20X DoubleQuick DNA Electrophoresis Buffer
73530-1
Sisco Laboratories
500 ml
EUR 64.4
Description: Part E
ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1
GenDepot
Ea
EUR 100
Hi-Eco Mini Horizontal Electrophoresis K
LA851-1NO
EWC Diagnostics
1 unit
EUR 205.56
Description: Hi-Eco Mini Horizontal Electrophoresis K
Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611
Bio Basic
1 UNIT
EUR 383.11
Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613
Bio Basic
1 UNIT
EUR 487.51
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)
EDK614
Bio Basic
1 UNIT
EUR 520.92
10-well protein Vertical Electrophoresis Apparatus
EDK692
Bio Basic
1 UNIT
EUR 487.51
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)
E37F007
EnoGene
100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis
Agar, High Gel Strength
40100064-1
Bio-WORLD
-
EUR 108.46
Agar, High Gel Strength
40100064-2
Bio-WORLD
-
EUR 174.63
Agar, High Gel Strength
40100064-3
Bio-WORLD
-
EUR 330.62
Agar, High Gel Strength
40100064-4
Bio-WORLD
-
EUR 718.22
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH
ELE2300
Scientific Laboratory Supplies
EACH
EUR 1802.25
myGel Mini Electrophoresis System 230V (includes transformer)
BM0291
GenDepot
System
EUR 580
myGel Mini Electrophoresis System 230V (includes transformer)
E1101-E
Benchmark Scientific
1 PC
EUR 665.55
CometAssay Electrophoresis Starter Kit plus EU Power Supply
4250-050-ESK-PS2
Biotechne
1 Kit
EUR 4597.68
CometAssay Electrophoresis Starter Kit plus UK Power Supply
4250-050-ESK-PS3
Biotechne
1 Kit
EUR 4597.68
CometAssay Electrophoresis Starter Kit plus SZ Power Supply
4250-050-ESK-PS4
Biotechne
1 Kit
EUR 4597.68
SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)
E37F001
EnoGene
100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH
ELE2302
Scientific Laboratory Supplies
EACH
EUR 1891.35
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator
E1201
Benchmark Scientific
1 each
EUR 1001.7
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)
E1101-SK
Benchmark Scientific
1 each
EUR 727.06
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)
E1101-SK-E
Benchmark Scientific
1 PC
EUR 757.35
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input
E1201-E
Benchmark Scientific
1 each
EUR 1058.9
OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH
VS10WCES
Scientific Laboratory Supplies
EACH
EUR 1614.21
Experiments were conducted to test the electrophoresis system for the characterization of different monoclonal antibodies and serum of animals, analysis of antibodies expressed in cell culture and antibody stability evaluation.