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Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Posted by Troy

glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous.

Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach. Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges.

Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of ​​acid gel. This study provides an effective approach for separating isomer weight protein glycosylation drug charges, and quantitative exploring the site-specific N-glycans dynamics along with the different isomers charge.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

PCR-Restriction Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Characterization of Listeria monocytogenes isolates of Ready-to-Eat Foods, Food Processing Environmental and Clinical Samples in South Africa.

Listeria monocytogenes is a ubiquitous, intracellular foodborne pathogen responsible for invasive listeriosis. The ability of L. monocytogenes causes the disease has some correlation with serotype specific group of offspring, making identification of the lineage is important for epidemiological analysis. Development of methods for connecting strain typing for outbreak of listeriosis L. monocytogenes will help minimize the spread of disease. The purpose of this study was to design a PCR-restriction fragment length polymorphism (RFLP) method for distinguishing between groups of lineages of L. monocytogenes.

PCR-amplified gene fragment hly for 12 serotypes of L. monocytogenes were sequenced, aligned, and analyzed by Bioedit program, and single nucleotide polymorphisms (SNPs) in the gene region identified. Because of the difficulty in obtaining serotype reference strain 4AB, these serotypes not included in this study. We tested the specificity and accuracy of PCR-RFLP method in the reference strains of L. monocytogenes and validated the method with 172 L. monocytogenes strains recovered from humans, food, environmental and food processing in 2000 to 2002 and from 2008 to 2010 from regions in Africa South.

PCR-RFLP analysis applied in this study L. monocytogenes serotype were placed into one of three groups based on different lineages and SNP sequences each lineage group. SNP preserved in areas where RFLP analysis can be applied to the difference between L. monocytogenes groups.This genealogical studies were conducted to evaluate the implementation of the original agarose gel electrophoresis reported previously for analysis of various monoclonal and polyclonal antibodies.

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Agarose, low EEO

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Agarose, medium EEO

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AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
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Description: Bioscience Mol Bio; Agarose

Agar, low gel strength

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Agar, low gel strength

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Agar, high gel strength

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Agar, high gel strength

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Agar, low gel strength, suitable for microbiology

GC6234-1KG 1 kg
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Agar, low gel strength, suitable for microbiology

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Agar, low gel strength, suitable for microbiology

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Agarose, Low melting gel

AB0015 10g
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HyAgarose? 3:1 Agarose, 100g

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Agarose for DNA electrophoresis

16-0027-500 500 g
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Agarose, medium EEO

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EUR 429

HyAgarose? HR Agarose, PCR Grade, 100g

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AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
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Description: Bioscience Mol Bio; Agarose

Agarose 3:1, 100g

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Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 54

HEPPS, 100g

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ES 6 Tooth Gel Comb For ES Electrophoresis

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Gentamycin Sulfate, 100g

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Kanamycin Sulfate, 100g

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Carbenicillin Disodium, 100g

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Ampicillin Sodium, 100g

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ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
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CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
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AGAR, HIGH GEL

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A01-102IHG-2Kg 2 Kg
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A01-102IHG-500g 500 g
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AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
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Description: Bioscience Mol Bio; Agarose

Hi-Bind? T-Gel-Agarose

M1313-1
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Hi-Bind? T-Gel-Agarose

M1313-10
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Hi-Bind? T-Gel-Agarose

M1313-100
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abx082006-100g 100 g
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Agarose

abx082007-100g 100 g
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Agarose

abx082008-100g 100 g
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CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 570.8

HiQTM Low Melting Agarose

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HiQTM Low Melting Agarose

A0223-010 100g
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AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 3737
Description: Bioscience Mol Bio; Agarose

Agarose StandardRoomTemperature

FYA002-100G 100 g Ask for price

Agarose Gel DNA Extraction and Purification

EP10010 100 Tests
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Starch (Smithies), for electrophoresis

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EUR 309

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 181

Agarose GPG/LMP low melt

A2501-100000 100 g
EUR 425
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Agarose GPG/LMP low melt

A2501-25000 25 g
EUR 154
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Agarose II, Low Gelling Temperature

CH002 25 g
EUR 229

Agarose II, Low Gelling Temperature

CH003 100 g
EUR 438

Agarose gel DNA extraction and purification kit

K1441-100
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110200-NG 5 ml
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E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies

EC11-G 1 ml
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Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies

KLH11-G 1 ml
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NAT1054 100G
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NAT1090 100G
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NAT1500 100G
EUR 165

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NAT1526 100G
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Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies

KLH11-G-5 5 ml
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dam for 15cm gel

EHS3400-DAM ea
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EHS3600-DAM ea
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ToxOut™ High Capacity Endotoxin Removal Agarose

7942-5 Ask for price

CHAPS, High Purity

1545-100G
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Rabbit Anti-E. Coli Proteins IgG-Agarose affinity gel for removing E. coli proteins

EC12-AS 1 ml
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Cys-link agarose gel coupling buffer kit (5-10 peptides)

110400-CB 1 kit
EUR 225

100G AquaPor ES

NAT1028 100G
EUR 292

100G AquaPor LM

NAT1032 100G
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NAT1036 100G
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NAT1064 100G
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100G SDS Ultrapure

NAT1072 100G
EUR 93

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NAT1512 100G
EUR 400

100G p-Terphenyl

NAT1522 100G
EUR 253

100G Bis-MSB

NAT1530 100G
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AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

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Description: Bioscience Mol Bio; Agarose

Protein A-Agarose High-Binding 6% Highly Crosslinked

P9301-005 5ml
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Protein A-Agarose High-Binding 6% Highly Crosslinked

P9301-010 10ml
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Protein A-Agarose High-Binding 6% Highly Crosslinked

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Protein A-Agarose High-Binding 6% Highly Crosslinked

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Hydroxyethyl cellulose, low viscosity

GC0004-100G 100 g
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EVS3100-BASE ea
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gel casting base for evs3200

EVS3200-BASE ea
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gel casting base for evs3300

EVS3300-BASE ea
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Gel tray V (for Visirays)

2195934 2unit
EUR 297
Description: Opt i on

Gel carrier for AE-6541

2394140 10unit
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Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2

G2008-050 500g
EUR 288

Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2

G2008-100 1Kg
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Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2

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CometAssay Electrophoresis System

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EUR 2950.8

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Description: Minimum order quantity: 1 unit of 100G

Hydroxyethyl cellulose, very low viscosity

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Carboxymethylcellulose sodium salt, low viscosity

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Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

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Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-100 1Kg
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Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-500 5Kg
EUR 1751

Agarose

20-abx186369
  • EUR 509.00
  • EUR 300.00
  • 100 g
  • 25 g

Agarose

20-abx098147
  • EUR 217.00
  • EUR 105.00
  • 100 g
  • 1 g

Agarose

G060-1 100 g
EUR 176

Agarose

G060-2 500 g
EUR 369

Agarose

Z5050007 500 g
EUR 615
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

Agarose

Z5050013 100 g
EUR 189
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time.

100G AquaPor LE GTAC

NAT1022 100G
EUR 209

100G AquaPor 3:1

NAT1040 100G
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100G Naphthalene -- Scintillation Grade

NAT1516 100G
EUR 75

Rabbit Gamma Globulin 100g

21005-7 100g
EUR 2370.5

Goat Gamma Globulin 100g

22005-4 100g
EUR 2628.24

Bovine Gamma Globulin 100g

27005-2 100g
EUR 477.16

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 185

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1587

supporting mask for midi gel dryer

EDRY1100-MASK ea
EUR 301

ssilicone rubber for midi gel dryer

EDRY1100-SILRUB ea
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supporting mask for maxi gel dryer

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EUR 479

silicone rubber for maxi gel dryer

EDRY1200-SILRUB ea
EUR 141

Gel Casting S for WSE-1710

2322107 4unit
EUR 283
Description: Gel Tr ay (S) x4, Gel Cast i ng St and (S), S si zecomb (9wel l /5wel l ) x2

Gel Casting L for WSE-1710

2322108 4unit
EUR 283
Description: Gel Tr ay (L ) x2, Gel Cast i ng St and (L ), S si zecomb (22wel l /12wel l ) x2

Gel Casting set for 10.5x6cm gels

E1101-CS1 1 PC
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Gel Casting Stand for 5x6cm gels

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Gel-Bright LED Gel Illuminator

E90003 1EA
EUR 773
Description: Minimum order quantity: 1 unit of 1EA

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
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ES Horizontal Electrophoresis Set

LE1011 Set
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myGel Mini Electrophoresis System

BM0286 System
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CometAssay Electrophoresis Starter Kit

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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

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Chitosan (5 cps), ultra low molecular weight

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Chitosan (100 - 300 cps), low molecular weight

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Chitosan (30 - 100 cps), low molecular weight

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Chitosan (10 cps), very low molecular weight

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Chitosan (1000 - 2000 cps), high molecular weight

GP5053-100G 100 g
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Precast Agarose Gel TAE, 2%, 12 Well, EtBr Stained, 20 gels per Box

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Precast Agarose Gel TAE, 2%, 17 Well, EtBr Stained, 20 gels per Box

TAE17-2PER-EB 1BOX, 20UNIT
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Precast Agarose Gel TAE, 2%, 48 Well, EtBr Stained, 10 gels per Box

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Precast Agarose Gel TBE, 2%, 12 Well, EtBr Stained, 20 gels per Box

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Precast Agarose Gel TBE, 2%, 48 Well, EtBr Stained, 10 gels per Box

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Gel-FAST? Gel Staining/Destaining Kit

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Rabbit THYMUS ACETONE POWDER 100g

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CK PANCREAS POWDER LYOPH 100g*

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MEI AGAR- 100G 500G,1KG

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MEI AGAR- 100G 500G,1KG

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MEI AGAR- 100G 500G,1KG

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TC Gel

CP048-005 500 g
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TC Gel

CP048-010 1 Kg
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Gel Tray

2394250 4unit
EUR 270
Description: I ncl udi ng pl at es

Gel Tray

2398194 2unit
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Gel Cutter

KS071012-11 12
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Gel Filter

KS071012-13 12
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Casting Dams, For Enduro 7cm Gel System

LE1000CD-01 Ea
EUR 126

Casting Dams, For Enduro 10cm Gel System

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Casting Dams, For Enduro 15cm Gel System

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Agarose A

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Agarose, Sepro

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Agarose, Sepro

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Agarose, Sepro

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Agarose Tablets

BA50201 100pcs
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Agarose M

AB0013 50g
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Agarose L.M.P

20-abx082386
  • EUR 272.00
  • EUR 217.00
  • 10 g
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Agarose LE

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EUR 116

Agarose LE

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EUR 291

Agarose HR

A-1280-100 100 g
EUR 183

Agarose HR

A-1280-500 500 g
EUR 685

Agarose GA

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Agarose GA

A-1290-500 500 g
EUR 255

Agarose LM

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Agarose LM

A-1300-25 25 g
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Chitosan (2000 - 3500 cps), very high molecular weight

GP8689-100G 100 g
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PBST buffer 10x Strength Solution

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TBST buffer 10x Strength Solution

PW009 500ml
EUR 65.66

Precast Agarose Gel TAE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box

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Precast Agarose Gel TAE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box

TAE17-2PER-SF 1BOX, 20UNIT
EUR 286.2

Precast Agarose Gel TAE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

TAE48-2PER-SF 1BOX, 10UNIT
EUR 366.68

Precast Agarose Gel TBE, 2%, 12 Well, Eco-Green Stained, 20 gels per Box

TBE12-2PER-SF 1BOX, 20UNIT
EUR 286.2

Precast Agarose Gel TBE, 2%, 17 Well, Eco-Green Stained, 20 gels per Box

TBE17-2PER-SF 1BOX, 20UNIT
EUR 286.2

Precast Agarose Gel TBE, 2%, 48 Well, Eco-Green Stained, 10 gels per Box

TBE48-2PER-SF 1BOX, 10UNIT
EUR 366.68

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 479

Experiments were conducted to test the electrophoresis system for the characterization of different monoclonal antibodies and serum of animals, analysis of antibodies expressed in cell culture and antibody stability evaluation.

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