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Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Posted by Troy

glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous.

Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach. Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges.

Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of ​​acid gel. This study provides an effective approach for separating isomer weight protein glycosylation drug charges, and quantitative exploring the site-specific N-glycans dynamics along with the different isomers charge.

Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.
Site-specific and Quantitative N-glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-resolution 2-dimensional Gel Electrophoresis and Mass Spectrometry.

PCR-Restriction Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Characterization of Listeria monocytogenes isolates of Ready-to-Eat Foods, Food Processing Environmental and Clinical Samples in South Africa.

Listeria monocytogenes is a ubiquitous, intracellular foodborne pathogen responsible for invasive listeriosis. The ability of L. monocytogenes causes the disease has some correlation with serotype specific group of offspring, making identification of the lineage is important for epidemiological analysis. Development of methods for connecting strain typing for outbreak of listeriosis L. monocytogenes will help minimize the spread of disease. The purpose of this study was to design a PCR-restriction fragment length polymorphism (RFLP) method for distinguishing between groups of lineages of L. monocytogenes.

PCR-amplified gene fragment hly for 12 serotypes of L. monocytogenes were sequenced, aligned, and analyzed by Bioedit program, and single nucleotide polymorphisms (SNPs) in the gene region identified. Because of the difficulty in obtaining serotype reference strain 4AB, these serotypes not included in this study. We tested the specificity and accuracy of PCR-RFLP method in the reference strains of L. monocytogenes and validated the method with 172 L. monocytogenes strains recovered from humans, food, environmental and food processing in 2000 to 2002 and from 2008 to 2010 from regions in Africa South.

PCR-RFLP analysis applied in this study L. monocytogenes serotype were placed into one of three groups based on different lineages and SNP sequences each lineage group. SNP preserved in areas where RFLP analysis can be applied to the difference between L. monocytogenes groups.This genealogical studies were conducted to evaluate the implementation of the original agarose gel electrophoresis reported previously for analysis of various monoclonal and polyclonal antibodies.

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6793.2

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis power supply

EV245 ea
EUR 574.8

miniONE Electrophoresis System

ELE3400 EACH
EUR 691.2

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

myGel Mini Electrophoresis System

E1101 1 each
EUR 623.9

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 651.6

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 854.4

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

Agar, high gel strength

GK9085-100G 100 g
EUR 64.8

Agar, high gel strength

GK9085-1KG 1 kg
EUR 208.8

Agar, high gel strength

GK9085-250G 250 g
EUR 93.6

Agar, high gel strength

GK9085-500G 500 g
EUR 134.4

Agar, high gel strength

GK9085-1 1
EUR 142.4

Agar, high gel strength

GK9085-100 100
EUR 23.8

Agar, high gel strength

GK9085-250 250
EUR 47.4

Agar, high gel strength

GK9085-500 500
EUR 81.5

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1602

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

Agar, low gel strength

GK2227-100G 100 g
EUR 60

Agar, low gel strength

GK2227-1KG 1 kg
EUR 170.4

Agar, low gel strength

GK2227-250G 250 g
EUR 79.2

Agar, low gel strength

GK2227-500G 500 g
EUR 112.8

Agar, low gel strength

GK2227-1 1
EUR 110.7

Agar, low gel strength

GK2227-100 100
EUR 19.8

Agar, low gel strength

GK2227-250 250
EUR 35.5

Agar, low gel strength

GK2227-500 500
EUR 63.3

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 712.8

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1434.85

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

Agar, low gel strength, suitable for microbiology

GC6234-100G 100 g
EUR 64.8

Agar, low gel strength, suitable for microbiology

GC6234-1KG 1 kg
EUR 184.8

Agar, low gel strength, suitable for microbiology

GC6234-250G 250 g
EUR 84

Agar, low gel strength, suitable for microbiology

GC6234-500G 500 g
EUR 118.8

Agar, low gel strength, suitable for microbiology

GC6234-1 1
EUR 122.6

Agar, low gel strength, suitable for microbiology

GC6234-100 100
EUR 23.8

Agar, low gel strength, suitable for microbiology

GC6234-250 250
EUR 39.4

Agar, low gel strength, suitable for microbiology

GC6234-500 500
EUR 68.9

Agarose, high EEO

GE7747-1 1
EUR 660.6

Agarose, high EEO

GE7747-250 250
EUR 227.8

Agarose, low EEO

GE0001-100G 100 g
EUR 160.8

Agarose, low EEO

GE0001-1KG 1 kg
EUR 724.8

Agarose, low EEO

GE0001-250G 250 g
EUR 265.2

Agarose, low EEO

GE0001-25G 25 g
EUR 84

Agarose, low EEO

GE0001-500G 500 g
EUR 418.8

Agarose, low EEO

GE0001-5G 5 g
EUR 58.8

Agarose, low EEO

GE0001-1 1
EUR 569.5

Agarose, low EEO

GE0001-100 100
EUR 102.7

Agarose, low EEO

GE0001-25 25
EUR 39.4

Agarose, low EEO

GE0001-250 250
EUR 189.8

Agarose, low EEO

GE0001-5 5
EUR 19

Agarose, low EEO

GE0001-500 500
EUR 316.4

Electrode Filling Sol Low Ionic Strength Applications

EFSLIAPP 100ML
EUR 101.84

Agarose LE Low EEO

AXY1000 PK10
EUR 2276.4

Agarose LE Low EEO

AXY1002 PK10
EUR 9276

Agarose B, Low EEO

AB0014 50g
EUR 99.67

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT

AGR-LE-100 1/pk
EUR 201.6
Description: Bioscience Mol Bio; Agarose

Benchmark Agarose LM, Low Melt, 100g

A1801-LM 1 PC
EUR 377.1

Agarose, Low melting gel

AB0015 10g
EUR 96.54

HyAgarose? LM Agarose, Low Melting, 100g

R9012LM-100g each
EUR 452.4

Low Level TISAB II Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes

IS-BUF3-500 500ML
EUR 156

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 217.2

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE

AGR-LE-1001 1/pk
EUR 926.4
Description: Bioscience Mol Bio; Agarose

Agarose, low EEO, GlenBiol™, suitable for molecular biology

GE6258-100 100
EUR 150.4

Agarose, low EEO, GlenBiol™, suitable for molecular biology

GE6258-25 25
EUR 60.1

Agarose 3:1, 100g

A0226-010 100g
EUR 578.4

Benchmark Agarose LE, 100g

A1701 1 PC
EUR 135.8

Kimas high strength centrifuge tubes

Z514861-6EA PK6
EUR 103.2

Agarose, medium EEO

GE4750-100G 100 g
EUR 199.2

Agarose, medium EEO

GE4750-500G 500 g
EUR 514.8

Agarose, medium EEO

GE4750-100 100
EUR 134.4

Agarose, medium EEO

GE4750-25 25
EUR 53.8

Agarose, medium EEO

GE4750-500 500
EUR 395.5

Benchmark Agarose 3:1, 100g

A1801-31 1 PC
EUR 287

HyAgarose? 3:1 Agarose, 100g

R901231-100g each
EUR 398.4

5M Sodium Nitrate Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes

IS-BUF1-500 500ML
EUR 158.4

10M Sodium Hydroxide Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes

IS-BUF2-500 500ML
EUR 184.8

AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE

AGR-LE-5001 1/pk
EUR 3669.6
Description: Bioscience Mol Bio; Agarose

HyAgarose? HR Agarose, PCR Grade, 100g

R9012HR-100g each
EUR 398.4

HyAgarose? LE Agarose, Multi-purpose, 100g

R9012LE-100g each
EUR 195.6

HyAgarose? LM Agarose, Low Melting, 500g

R9012LM-500g each
EUR 1821.6

Kimax Reuseable Glass Centrifuge tubes plain high strength 15ml

TUB2718 PK6
EUR 122.4

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

HyAgarose? LM Agarose, Low Melting, 10g

R9012LM-10g each
EUR 120

HyAgarose? LM Agarose, Low Melting, 50g

R9012LM-50g each
EUR 262.8

Agarose II, Low Gelling Temperature

CH002 25 g
EUR 274.8

Agarose II, Low Gelling Temperature

CH003 100 g
EUR 525.6

Agarose LM Low Melting

AXY1004 PK10
EUR 11359.2

Agarose LM Low Melting

AXY1006 PK10
EUR 6266.4

Benchmark Agarose HR, PCR Grade for DNA fragments between 20 to 800bp, 100g

A1801-HR 1 PC
EUR 326.1

HiQTM Low Melting Agarose

A0223-002 25g
EUR 343.2

HiQTM Low Melting Agarose

A0223-010 100g
EUR 699.6

EZ Pack™ Agarose Tablets, pack of 200 tablets (100g)

A2501 1 PC
EUR 188.5

Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-050 500g
EUR 360

Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-100 1Kg
EUR 562.8

Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2

G2007-500 5Kg
EUR 2101.2

Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2

G2008-050 500g
EUR 345.6

Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2

G2008-100 1Kg
EUR 549.6

Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2

G2008-500 5Kg
EUR 2004

Agarose GPG/LMP low melt

A2501-100000 100 g
EUR 510
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Agarose GPG/LMP low melt

A2501-25000 25 g
EUR 184.8
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays.

Agarose Gel DNA Extraction and Purification

EP10010 100 Tests
EUR 356.4

ToxOut™ High Capacity Endotoxin Removal Agarose

7942-5 each Ask for price

Apex Low Melt Agarose Ultra Pure

20-103 25g/Unit
EUR 375

Apex Low Melt Agarose Ultra Pure

20-104 100g/Unit
EUR 687

AGAROSE 25G-125G -500G

A01-118-10kg 10 kg Ask for price

AGAROSE 25G-125G -500G

A01-118-2kg 2kg Ask for price

AGAROSE 25G-125G -500G

A01-118-500g 500 g
EUR 592.8

Benchmark Agarose LE, 500g

A1705 1 PC
EUR 473.2

Agarose gel DNA extraction and purification kit

K1441-100 each
EUR 510

Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins)

110200-NG 5 ml
EUR 343.2

Agarose IEF 10g

17046801 EACH
EUR 266.4

Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins)

110100-CG 5 ml
EUR 343.2

HyAgarose? 3:1 Agarose, 500g

R901231-500g each
EUR 1369.2

Apex Low Melt Sieve Agarose Ultra Pure

20-245 100g/Unit
EUR 566

Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies

KLH11-G 1 ml
EUR 270

AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE

AGR-MS-1001 1/pk
EUR 2445.6
Description: Bioscience Mol Bio; Agarose

Benchmark Agarose LE, 25g

A1700 1 PC
EUR 56.3

Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies

KLH11-G-5 5 ml
EUR 634.8

GelRED(TM) for Agarose or Page Gels (10000X in water)

S420 0,5 ml
EUR 175.2

GelRED(TM) for Agarose or Page Gels (10000X in water)

S425 5x0,5 ml
EUR 699.6

GelRED(TM) for Agarose or Page Gels (10000X in water)

S425L 15x0,5 ml Ask for price

Cys-link agarose gel coupling buffer kit (5-10 peptides)

110400-CB 1 kit
EUR 270

PBST buffer 10x Strength Solution

PW004 500ml
EUR 80.88

TBST buffer 10x Strength Solution

PW009 500ml
EUR 78.79

Carazzi Haematoxylin (Double Strength)

RRSP61-D 500ml
EUR 15.6

Carazzi Haematoxylin (Double Strength)

RRSP61-E 1L
EUR 26.23

Carazzi Haematoxylin (Double Strength)

RRSP61-F 2.5L
EUR 53.3

GelRed Agarose LE

41029-50G 50G
EUR 384
Description: Minimum order quantity: 1 unit of 50G

GelRed Agarose LE

41029-5G 5G
EUR 124.8
Description: Minimum order quantity: 1 unit of 5G

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2490
Description: Bioscience Mol Bio; Agarose

Experiments were conducted to test the electrophoresis system for the characterization of different monoclonal antibodies and serum of animals, analysis of antibodies expressed in cell culture and antibody stability evaluation.

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