glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous.
Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach. Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges.
Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of ​​acid gel. This study provides an effective approach for separating isomer weight protein glycosylation drug charges, and quantitative exploring the site-specific N-glycans dynamics along with the different isomers charge.
PCR-Restriction Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis Characterization of Listeria monocytogenes isolates of Ready-to-Eat Foods, Food Processing Environmental and Clinical Samples in South Africa.
Listeria monocytogenes is a ubiquitous, intracellular foodborne pathogen responsible for invasive listeriosis. The ability of L. monocytogenes causes the disease has some correlation with serotype specific group of offspring, making identification of the lineage is important for epidemiological analysis. Development of methods for connecting strain typing for outbreak of listeriosis L. monocytogenes will help minimize the spread of disease. The purpose of this study was to design a PCR-restriction fragment length polymorphism (RFLP) method for distinguishing between groups of lineages of L. monocytogenes.
PCR-amplified gene fragment hly for 12 serotypes of L. monocytogenes were sequenced, aligned, and analyzed by Bioedit program, and single nucleotide polymorphisms (SNPs) in the gene region identified. Because of the difficulty in obtaining serotype reference strain 4AB, these serotypes not included in this study. We tested the specificity and accuracy of PCR-RFLP method in the reference strains of L. monocytogenes and validated the method with 172 L. monocytogenes strains recovered from humans, food, environmental and food processing in 2000 to 2002 and from 2008 to 2010 from regions in Africa South.
PCR-RFLP analysis applied in this study L. monocytogenes serotype were placed into one of three groups based on different lineages and SNP sequences each lineage group. SNP preserved in areas where RFLP analysis can be applied to the difference between L. monocytogenes groups.This genealogical studies were conducted to evaluate the implementation of the original agarose gel electrophoresis reported previously for analysis of various monoclonal and polyclonal antibodies.
 Agar, high gel strength |
|||
| GK9085-100G | Glentham Life Sciences | 100 g | EUR 54 |
 Agarose, low EEO |
|||
| GE0001-1KG | Glentham Life Sciences | 1 kg | EUR 604 |
Agarose, low EEO |
|||
| GE0001-250G | Glentham Life Sciences | 250 g | EUR 221 |
Agarose, low EEO |
|||
| GE0001-25G | Glentham Life Sciences | 25 g | EUR 70 |
Agarose, low EEO |
|||
| GE0001-500G | Glentham Life Sciences | 500 g | EUR 349 |
Agarose, low EEO |
|||
| GE0001-5G | Glentham Life Sciences | 5 g | EUR 49 |
 Agarose, low EEO, GlenBiol, suitable for molecular biology |
|||
| GE6258-100G | Glentham Life Sciences | 100 g | EUR 181 |
 Agar, low gel strength, suitable for microbiology |
|||
| GC6234-100G | Glentham Life Sciences | 100 g | EUR 54 |
 HyAgarose? LM Agarose, Low Melting, 100g |
|||
| R9012LM-100g | ACTGene | EUR 377 | |
 Agarose B, Low EEO |
|||
| AB0014 | Bio Basic | 50g | EUR 83.06 |
|
|||
 Agarose, medium EEO |
|||
| GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
 AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT |
|||
| AGR-LE-100 | CORNING | 1/pk | EUR 168 |
|
Description: Bioscience Mol Bio; Agarose |
|||
 Agar, low gel strength |
|||
| GK2227-1KG | Glentham Life Sciences | 1 kg | EUR 142 |
Agar, low gel strength |
|||
| GK2227-250G | Glentham Life Sciences | 250 g | EUR 66 |
Agar, low gel strength |
|||
| GK2227-500G | Glentham Life Sciences | 500 g | EUR 94 |
Agar, high gel strength |
|||
| GK9085-1KG | Glentham Life Sciences | 1 kg | EUR 174 |
Agar, high gel strength |
|||
| GK9085-250G | Glentham Life Sciences | 250 g | EUR 78 |
Agar, high gel strength |
|||
| GK9085-500G | Glentham Life Sciences | 500 g | EUR 112 |
 Agarose, Low melting gel |
|||
| AB0015 | Bio Basic | 10g | EUR 80.45 |
|
|||
Agar, low gel strength, suitable for microbiology |
|||
| GC6234-1KG | Glentham Life Sciences | 1 kg | EUR 154 |
Agar, low gel strength, suitable for microbiology |
|||
| GC6234-250G | Glentham Life Sciences | 250 g | EUR 70 |
Agar, low gel strength, suitable for microbiology |
|||
| GC6234-500G | Glentham Life Sciences | 500 g | EUR 99 |
 HyAgarose? 3:1 Agarose, 100g |
|||
| R901231-100g | ACTGene | EUR 332 | |
 Agarose for DNA electrophoresis |
|||
| 16-0027-500 | Medicago | 500 g | EUR 342 |
Agarose, medium EEO |
|||
| GE4750-500G | Glentham Life Sciences | 500 g | EUR 429 |
 AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE |
|||
| AGR-LE-1001 | CORNING | 1/pk | EUR 772 |
|
Description: Bioscience Mol Bio; Agarose |
|||
 HyAgarose? HR Agarose, PCR Grade, 100g |
|||
| R9012HR-100g | ACTGene | EUR 332 | |
 HyAgarose? LE Agarose, Multi-purpose, 100g |
|||
| R9012LE-100g | ACTGene | EUR 163 | |
 Agarose 3:1, 100g |
|||
| A0226-010 | GenDepot | 100g | EUR 482 |
 Acrylamide, suitable for electrophoresis |
|||
| GK5651-100G | Glentham Life Sciences | 100 g | EUR 54 |
 HEPPS, 100g |
|||
| R1605-100g | ACTGene | EUR 190 | |
 ES 6 Tooth Gel Comb For ES Electrophoresis |
|||
| LE1011-A4 | GenDepot | Ea | EUR 124 |
 Gentamycin Sulfate, 100g |
|||
| R1405-100g | ACTGene | EUR 541 | |
 Kanamycin Sulfate, 100g |
|||
| R2538-100g | ACTGene | EUR 471 | |
 Carbenicillin Disodium, 100g |
|||
| R4800-100g | ACTGene | EUR 977 | |
 Ampicillin Sodium, 100g |
|||
| R6952-100g | ACTGene | EUR 260 | |
 ES 8/10 Double Gel Comb For ES Electrophoresis |
|||
| LE1011-A3 | GenDepot | Ea | EUR 124 |
 CometAssay Single Cell Gel Electrophoresis Assay |
|||
| 4250-050-K | Biotechne | 50 tests | EUR 428 |
 AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE |
|||
| AGR-LE-5001 | CORNING | 1/pk | EUR 3058 |
|
Description: Bioscience Mol Bio; Agarose |
|||
 AGAR, HIGH GEL |
|||
| A01-102IHG-10kg | Alphabiosciences | 10 kg | EUR 2219 |
AGAR, HIGH GEL |
|||
| A01-102IHG-2Kg | Alphabiosciences | 2 Kg | EUR 521 |
AGAR, HIGH GEL |
|||
| A01-102IHG-500g | Alphabiosciences | 500 g | EUR 178 |
 Hi-Bind? T-Gel-Agarose |
|||
| M1313-1 | Biovision | EUR 131 | |
Hi-Bind? T-Gel-Agarose |
|||
| M1313-10 | Biovision | EUR 218 | |
Hi-Bind? T-Gel-Agarose |
|||
| M1313-100 | Biovision | EUR 1501 | |
 Agarose |
|||
| abx082006-100g | Abbexa | 100 g | EUR 244 |
|
|||
Agarose |
|||
| abx082007-100g | Abbexa | 100 g | EUR 258 |
|
|||
Agarose |
|||
| abx082008-100g | Abbexa | 100 g | EUR 230 |
|
|||
 CometAssay Single Cell Gel Electrophoresis Assay-Silver |
|||
| 4251-050-K | Biotechne | 1 kit | EUR 570.8 |
 HiQTM Low Melting Agarose |
|||
| A0223-002 | GenDepot | 25g | EUR 286 |
HiQTM Low Melting Agarose |
|||
| A0223-010 | GenDepot | 100g | EUR 583 |
) AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL) |
|||
| AGR-LM-1001 | CORNING | 1/pk | EUR 3737 |
|
Description: Bioscience Mol Bio; Agarose |
|||
 Agarose Gel DNA Extraction and Purification |
|||
| EP10010 | Neuromics | 100 Tests | EUR 297 |
 Agarose StandardRoomTemperature |
|||
| FYA002-100G | Yeastern Biotech | 100 g | Ask for price |
 Agarose GPG/LMP low melt |
|||
| A2501-100000 | ApexBio | 100 g | EUR 425 |
|
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays. |
|||
Agarose GPG/LMP low melt |
|||
| A2501-25000 | ApexBio | 25 g | EUR 154 |
|
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays. |
|||
 Agarose gel DNA extraction and purification kit |
|||
| K1441-100 | Biovision | EUR 425 | |
, for electrophoresis) Starch (Smithies), for electrophoresis |
|||
| GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 309 |
Starch (Smithies), for electrophoresis |
|||
| GE6857-500G | Glentham Life Sciences | 500 g | EUR 181 |
) Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins) |
|||
| 110100-CG | Alpha Diagnostics | 5 ml | EUR 286 |
) Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins) |
|||
| 110200-NG | Alpha Diagnostics | 5 ml | EUR 286 |
 E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies |
|||
| EC11-G | Alpha Diagnostics | 1 ml | EUR 286 |
-Agarose affinity gel for removing KLH antibodies) Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies |
|||
| KLH11-G | Alpha Diagnostics | 1 ml | EUR 225 |
 Dried Aluminium Hydroxide Gel |
|||
| GK2734-100G | Glentham Life Sciences | 100 g | EUR 46 |
 HyAgarose? LM Agarose, Low Melting, 10g |
|||
| R9012LM-10g | ACTGene | EUR 100 | |
 HyAgarose? LM Agarose, Low Melting, 500g |
|||
| R9012LM-500g | ACTGene | EUR 1518 | |
 HyAgarose? LM Agarose, Low Melting, 50g |
|||
| R9012LM-50g | ACTGene | EUR 219 | |
 ToxOut? High Capacity Endotoxin Removal Agarose |
|||
| 7942-5 | Biovision | EUR 262 | |
 100G Acrylamide |
|||
| NAT1014 | National Diagnostics | 100G | EUR 100 |
 100G Tricine |
|||
| NAT1054 | National Diagnostics | 100G | EUR 100 |
 100G EDTA |
|||
| NAT1090 | National Diagnostics | 100G | EUR 79 |
 100G PPO |
|||
| NAT1500 | National Diagnostics | 100G | EUR 165 |
 100G POPOP |
|||
| NAT1526 | National Diagnostics | 100G | EUR 420 |
-Agarose af is finity gel for removing KLH antibodies) Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies |
|||
| KLH11-G-5 | Alpha Diagnostics | 5 ml | EUR 529 |
 dam for 20cm gel |
|||
| EHS3500-DAM | Consort | ea | EUR 166 |
 dam for 23.5cm gel |
|||
| EHS3600-DAM | Consort | ea | EUR 166 |
 dam for 15cm gel |
|||
| EHS3400-DAM | Consort | ea | EUR 158 |
 Rabbit Anti-E. Coli Proteins IgG-Agarose affinity gel for removing E. coli proteins |
|||
| EC12-AS | Alpha Diagnostics | 1 ml | EUR 529 |
) Cys-link agarose gel coupling buffer kit (5-10 peptides) |
|||
| 110400-CB | Alpha Diagnostics | 1 kit | EUR 225 |
 100G AquaPor ES |
|||
| NAT1028 | National Diagnostics | 100G | EUR 292 |
 100G AquaPor LM |
|||
| NAT1032 | National Diagnostics | 100G | EUR 717 |
 100G AquaPor HR |
|||
| NAT1036 | National Diagnostics | 100G | EUR 494 |
 100G Ammonium Persulfate |
|||
| NAT1064 | National Diagnostics | 100G | EUR 99 |
 100G SDS Ultrapure |
|||
| NAT1072 | National Diagnostics | 100G | EUR 93 |
 100G Butyl PBD |
|||
| NAT1512 | National Diagnostics | 100G | EUR 400 |
 100G p-Terphenyl |
|||
| NAT1522 | National Diagnostics | 100G | EUR 253 |
 100G Bis-MSB |
|||
| NAT1530 | National Diagnostics | 100G | EUR 497 |
 AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE |
|||
| AGR-MS-1001 | CORNING | 1/pk | EUR 2038 |
|
Description: Bioscience Mol Bio; Agarose |
|||
 CHAPS, High Purity |
|||
| 1545-100G | Biovision | EUR 637 | |
 Protein A-Agarose High-Binding 6% Highly Crosslinked |
|||
| P9301-005 | GenDepot | 5ml | EUR 228 |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
|||
| P9301-010 | GenDepot | 10ml | EUR 347 |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
|||
| P9301-050 | GenDepot | 50ml | EUR 1357 |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
|||
| P9301-100 | GenDepot | 100ml | EUR 2413 |
 Hydroxyethyl cellulose, low viscosity |
|||
| GC0004-100G | Glentham Life Sciences | 100 g | EUR 45 |
 Pectin, amidated, low ester |
|||
| GC7250-100G | Glentham Life Sciences | 100 g | EUR 65 |
 Chitosan, low molecular weight |
|||
| GC9009-100G | Glentham Life Sciences | 100 g | EUR 118 |
) Gel tray V (for Visirays) |
|||
| 2195934 | Atto | 2unit | EUR 297 |
|
Description: Opt i on |
|||
 Gel carrier for AE-6541 |
|||
| 2394140 | Atto | 10unit | EUR 253 |
Experiments were conducted to test the electrophoresis system for the characterization of different monoclonal antibodies and serum of animals, analysis of antibodies expressed in cell culture and antibody stability evaluation.
