Glycine Receptor Complex Analysis Using Immunoprecipitation-Blue Native Gel Electrophoresis-Mass Spectrometry.

Pentameric glycine receptor (GlyR), which consists of α1 and β subunits, is the major inhibitory ionotropic receptors in the brain stem and spinal cord. GlyRs interact with gephyrin (GPHN), a protein scaffold that anchors GlyR in the plasma membrane and allowing it to form a cluster in postsynapses glycinergic.

Using interaction proteomics approach, we provide evidence ArfGEFs IQ motif and domain Sec7 3 (IQSEC3) and IQ motif and domain Sec7 2 (IQSEC2) as two novel synaptic protein complexes interact with GlyR. When affinity-isolated GlyR complex fractionated with blue gel electrophoresis original and characterized by mass spectrometry, GlyR α1β-GPHN emerged as a complex of the most abundant with a molecular weight of about 1 MDA, and GlyR α1β-GPHN-IQSEC3 as minor proteins complex about 1, 2 MDA.

A third GlyR α1β-GPHN-IQSEC2 complex is on the lowest number with mass similar to a complex containing IQSEC3. Using yeast two-hybrid we show that IQSEC3 GlyR interact with the complex by binding to G GPHN domain at the N-terminal of IQSEC3 IQ-like domain. Our data provide direct evidence of the complex interaction with GlyR IQSEC3-GPHN, underscores the potential role of this ArfGEFs in synaptic function glycinergic. This article is protected by copyright. All rights reserved.

Glycine Receptor Complex Analysis Using Immunoprecipitation-Blue Native Gel Electrophoresis-Mass Spectrometry.
Glycine Receptor Complex Analysis Using Immunoprecipitation-Blue Native Gel Electrophoresis-Mass Spectrometry.

Quantitative Analysis of RNA Guard activities by Native Gel Electrophoresis and Fluorescence Spectroscopy.

various types of RNA binding proteins noncoding RNA chaperone interaction by increasing the level of RNA base pairs and to stabilize the RNA duplex end. E. coli protein Hfq facilitate interactions between noncoding small RNAs and their target mRNAs. Companion and RNA annealing activity of Hfq and other RNA chaperone can be evaluated by determining the kinetics of RNA base pairs in the presence and absence of protein.

This chapter presents a protocol for measuring RNA annealing kinetics using gel electrophoresis mobility shift tests (EMSA), to stop the flow of fluorescence, and fluorescence anisotropy. EMSA is low cost and can complete the reaction intermediates and natural small RNA fragments of mRNA, long enough for complex life (≥10 s) trapped during electrophoresis. Stop the flow of fluorescence can detect annealing reaction between 1 ms and 30 and most suitable for measuring rapid annealing oligoribonucleotides.

fluorescence anisotropy reported physical size of the complex and is suitable for monitoring the association and dissociation of RNA from Hfq for companion cycle.We measured bone-specific alkaline phosphatase (ALP) isoenzyme activity in 67 plasma samples from 14 newborn Holstein calves using both conventional methods ( featuring heat inactivation) and commercial agarose gel electrophoresis (AGE) kit; Relevant isoenzymes called bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3).

We explored whether AGE kit supplied reliable data when used to analyze samples from Holstein calves. Blood was collected from the jugular vein of each calf shortly before the first meal of colostrum (pre-feeding), 20 and 40 hours after the pre-meal, and on days 4 and 7; while three samples (from three calf) is not obtained. Plasma total ALP activity varied widely, exceeding the reference value range. In the electrophoresis, 52 of 67 samples of plasma (77.6%) clearly contains both ALP isoenzyme 2 and ALP3, as do human control serum.

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
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ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
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ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
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ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
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ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 975

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
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Starch (Smithies), for electrophoresis

GE6857-500G 500 g
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Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 54

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 342

Horizontal head

BV1000-H15 1 PC
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Horizontal head, for 12 x 1.5ml

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electrophoresis power supply

EV245 ea
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CometAssay Electrophoresis System

4250-050-ES 1 Kit
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ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
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6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
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myGel Mini Electrophoresis System

BM0286 System
EUR 618

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
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ES 8/10 Double Gel Comb For ES Electrophoresis

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mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 318

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EUR 352

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EUR 572

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EUR 775

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EUR 817

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EHS3600-SYS ea
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6 x 15ml horizontal rotisserie

R4040-HZ15 1 PC
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6 x 50ml horizontal rotisserie

R4040-HZ50 1 PC
EUR 164.69

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

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EUR 749

electrophoresis ps 1500v, 300ma, 150w

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electrophoresis ps 1500v, 300ma, 150w

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EUR 572

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EV2320 ea
EUR 749

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EV245-UK ea
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electrophoresis power supply 120 vac

EV245-US ea
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electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 572

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EV3020 ea
EUR 800

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EV3150 ea
EUR 800

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EV3150-UK ea
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EV3150-US ea
EUR 800

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1214

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 800

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EV3620 ea
EUR 1214

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EV3620-UK ea
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EUR 1214

5 x SDS Electrophoresis Buffer

GR103039 1 L
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AXYGEN® HORIZONTAL GEL BOX, 10 CM

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EUR 333
Description: Lab Equipment; Axygen Branded EQ

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Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

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Description: (90mm・r efer ence at t ached・1.5mm t i ck ness)

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Antibody for Phosphoserine

SPC-149F 0.4ml
EUR 362
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is unconjugated.

Antibody for Phosphoserine

SPC-149F-A390 0.4ml
EUR 409
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 390.

Antibody for Phosphoserine

SPC-149F-A488 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 488.

Antibody for Phosphoserine

SPC-149F-A565 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 565.

Antibody for Phosphoserine

SPC-149F-A594 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 594.

Antibody for Phosphoserine

SPC-149F-A633 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 633.

Antibody for Phosphoserine

SPC-149F-A655 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 655.

Antibody for Phosphoserine

SPC-149F-A680 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 680.

Antibody for Phosphoserine

SPC-149F-A700 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 700.

Antibody for Phosphoserine

SPC-149F-ALP 0.4ml
EUR 403
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Alkaline Phosphatase.

Antibody for Phosphoserine

SPC-149F-APC 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to APC .

Antibody for Phosphoserine

SPC-149F-APCCY7 0.4ml
EUR 480
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to APC/Cy7.

Antibody for Phosphoserine

SPC-149F-BI 0.4ml
EUR 405
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Biotin.

ALP activity a total of 52 samples ranging from 166-1989 U / L (median: 1013 U / L), while the value for the other 15 samples (22.4%) showed normal ranges fractionation isoenzymes 1014-5118 U / L (median: 1780 U / L).