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High-Throughput Analysis of Lignin by Agarose Gel Electrophoresis

High-Throughput Analysis of Lignin by Agarose Gel Electrophoresis
Posted by Troy

The analytic method of electrophor gel electrophoresis (age) has been developed to separate the lignin fraction according to the molecular weight (MW), charge, and shape. The operating conditions to influence species separation have been evaluated along with imaging parameters. Kraft, soda (protobind), and lignin organosolv show different differences in migration. The band is cut, extracted, and analyzed cross with gel permeation chromatography (GPC), 1H NMR, and Pyrolysis GC / MS to confirm their identity as lignin.

The tape intensity correlates with lignin concentration by running a diluted sample serially and imaging each path to produce the right calibration curve. Age techniques are used to monitor and compare enzymatic, bacteria, chemistry, and hydrothermal lignin digestions. Each method shows change in lignin migration and the intensity of the ribbon from time to time. Low MW species are seen in samples collected from the anode buffer tank. Although it requires further development, the age method can provide structural information about lignin and can be accessed by biological and chemical laboratories.

Analysis of West Blotting from proteins separated by Gelose’s original gel electrophoresis

West Blotting was tried to analyze proteins separated by the original gel electrophoresis so that previously developed on the MES system. This report shows a simple protocol to damage the original gel to become a PVDF membrane by soaking gel in the transfer buffer containing sodium dodecylsulfate and 3 examples of analysis. The first example shows the expression of recombinant antibodies in HEK293 cells with direct coloring from the original gel to protein and nucleic acid and script staining for proteins and host cell proteins.

These analyzes indicate the usefulness of the original gel electrophoresis wave, confirming that recombinant antibodies migrated towards the cathode while nucleic acid and the majority of host cell proteins migrated to the anode. The second example shows the state of the phosphorylation of map kinase on the lines of human lymphocytes. That is, the original gel agarose can separate kinases, phosphorylation that can be analyzed by West Bloting. The third example shows the correlation of escherichia coli β-galactosidase expressions between oligomeral activity and enzymes using antibodies and substrate coloring.

Label Electrophoresis gel high sensitivity protein compatible with mass spectrometry

Sodium Dodecyl Sulfate Electrophoresis Gel Poliakrilamide (SDS-Page) is a technique that is widely used for macromolecular and protein analysis. While some methods exist to visualize protein bands separated on gel, one of the most popular coloring methods of protein with coomassie dyes. A newer approach is to use Bio-rad stain technology to visualize protein bands with UV rays and achieve similar or greater sensitivity than Coomassie dyes. Here, we develop a method to further strengthen the sensitivity of the free stain gel using Carboxyfluorescent Succinimidyl Esther (CFSE) coloring.

We compare our novel method using fetal cow serum samples with dye coomassie, standard stain-free gel, and silver coloring. Our results show that while silver coloring remains a gold standard method in terms of sensitivity; Parnisan CFSE samples before use with stain-free gel produce increased sensitivity of 10-100-fold for Coomassie coloring and standard stain standard methods. Our method offers sensitivity similar to silver coloring that is compatible with downstream mass spectrometry, and is therefore more profitable for further recording and macromolecular analysis in the ribbon.

New quantitative gel electrophoresis method with image-based detection for determining food coloring and metallic ions

This work explains the alternative application of electrophoresis gel for separation and quantification of analytes with low molecular weight using innovative and low-cost equipment that allows acquisition of image-based electrofesterogram with webcam. As proof of concepts, determining CU and NI content in metal alloys are evaluated by means of separation and detection of metal ions, which were previously complex with Black T. Eriichrome, Determination of Sunset Yellow FCF food staining material, Tartrazine, brilliant blue FCF and Amaranth Red in refreshing samples Powder investigated as an alternative to a very stable method used for this purpose.

High-Throughput Analysis of Lignin by Agarose Gel Electrophoresis

For all investigated analysis, the appropriate electrophoretic peak indicates the signal ratio of noise ranges from 10 to 180, appropriate precision in the area (RSD <3.5%) and linear relationship (R> 0.99) between RGB detected signaled and Concentration of standard solutions. The application of methods for determining CU and NI content in metal alloys provide results without significant differences, at a 95% confidence level, when compared to the results obtained by the FAA-based method. A clear recovery is estimated to sample refresher powder enriched with food coloring ranging from 93% to 108% for the content and content found, indicating the absence of a matrix effect. Studies prove the feasibility of separation and color analytic quantification with gel electrophoresis and image-based detection that can be useful for different samples.

High resolution analysis ultrafast oligosacakaride human milk with multicapillary gel electrophoresis

There is a lot of interest that grows towards the synthesized (HMO) human milk oligosaccharide as a baby formula additive, and is also interesting as a food supplement for adults. At present some producers synthesize HMO, however, their analysis is challenging, both in resolution and speed. In this paper, the ultrafast high resolution method was introduced for HMO separation with multicapillary gel electrophoresis.

Two gel compositions are evaluated with complementary resolution forces. One is a matrix separation of carbohydrate carbohydrate standards of industry used conventionally, completing oligosaccharides according to their costs for hydrodynamic volume ratios. The other is a gel dextran buffer buffer, which utilizes a secondary balance of the complexity of powder-vicinal powder to increase resolution. Considering fast analytical time and multiplexing (12 channel systems), sample plates 96 can be analyzed in less than 80 minutes with conventional type carbohydrate separation matrix and in less than an hour with borat buffer buffer gel.

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

miniONE Electrophoresis System

ELE3400 EACH
EUR 691.2

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

myGel Mini Electrophoresis System

E1101 1 each
EUR 623.9

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6793.2

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis power supply

EV245 ea
EUR 574.8

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 854.4

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 74.66

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 139.51

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 17.3

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 23.23

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 35.42

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 62.99

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 712.8

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1434.85

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

HORIZONTAL TANK KIT

IB52200 -
EUR 55.88

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 2 x 50ml

BV1000-H500 1 PC
EUR 108.5

Horizontal head for 2 x 50mL

MIX7022 EACH
EUR 146.4

Horizontal MicroLoops E

M8-L18SP-15H 20pcs.
EUR 196.6

Horizontal MicroLoops E

M8-L18SP-50H 20pcs.
EUR 196.6

Midi Horizontal Gel Unit

ELE8120 EACH
EUR 630.42

TOOL REST, HORIZONTAL BAR

I4013 1EA
EUR 14.06

IBI QS710 HORIZONTAL UNIT

IB51000 7CM X 10CM FORMAT
EUR 509.62

IBI MP1015 HORIZONTAL UNIT

IB53000 10CM X 15CM FORMAT
EUR 675.93

IBI HR2025 HORIZONTAL UNIT

IB56000 20CM X 25CM FORMAT
EUR 877.32

IBI HR2525 HORIZONTAL UNIT

IB57000 25CM X 25CM FORMAT
EUR 965.82

Pipette Tray PVC Horizontal

PIP5500 EACH
EUR 44.4

Horizontal 50mL Tube Holder

MIX5088 EACH
EUR 176.4

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 381.6

mini horizontal unit 7x10 cm

EHS1102-SYS ea
EUR 381.6

midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 422.4

mini horizontal unit 9x11 cm

EHS3200-SYS ea
EUR 595.2

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
EUR 930

wide horizontal unit 23.5x25 cm

EHS3610-SYS ea
EUR 1030.8

Horizontal orientator 384 wells

DD53423 PK2
EUR 78

midi horizontal unit 10x10 cm

EHS1202-SYS ea
EUR 422.4

maxi horizontal unit 20x10 cm

EHS1401-SYS ea
EUR 686.4

maxi horizontal unit 20x20 cm

EHS1402-SYS ea
EUR 727.2

maxi horizontal unit 20x25 cm

EHS1403-SYS ea
EUR 747.6

mini horizontal unit 12x14 cm

EHS3300-SYS ea
EUR 625.2

midi horizontal unit 15x15 cm

EHS3400-SYS ea
EUR 757.2

midi horizontal unit 15x25 cm

EHS3410-SYS ea
EUR 930

midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 980.4

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 12 x 1.5ml

BV1000-H15 1 PC
EUR 108.5

Horizontal head for 12 x 1.5mL

MIX7018 EACH
EUR 146.4

HS 260 Basic Horizontal Shaker

3066602 EACH
EUR 3482.4

Axygen Horizontal Gel Box 20Cm

HGB-20 EACH
EUR 900

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 4 x 15ml

BV1000-H150 1 PC
EUR 108.5

Horizontal head for 4 x 15mL

MIX7020 EACH
EUR 146.4

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 433.2

INSTRUMENT REST, HORIZONTAL SLOTTED

I556 1EA
EUR 19.7

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
EUR 422.4

midiplus horizontal unit 15x10 cm

EHS1302-SYS ea
EUR 433.2

midiplus horizontal unit 15x15 cm

EHS1303-SYS ea
EUR 433.2

maxiplus horizontal unit 26x16 cm

EHS1501-SYS ea
EUR 939.6

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
EUR 939.6

maxiplus horizontal unit 26x32 cm

EHS1503-SYS ea
EUR 939.6

Horizontal MicroLoops E - Assortment

M8-L18SP-HA1 20pcs.
EUR 196.6

midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 453.6

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 583.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 343.2
Description: Lab Equipment; Axygen Branded EQ

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 727.2

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS ea
EUR 1132.8

MAGic Clamp Tube Rack 2 x 50mL tubes horizontal

MIX7176 EACH
EUR 163.2

6 x 15mL horizontal rotisserie 1 ea

MIX7080 EACH
EUR 92.4

6 x 50mL horizontal rotisserie 1 ea

MIX7082 EACH
EUR 92.4

6 x 15ml horizontal rotisserie, 1 ea

R4040-HZ15 1 PC
EUR 68

6 x 50ml horizontal rotisserie, 1 ea

R4040-HZ50 1 PC
EUR 68

Horizontal Plastic Clip Microtube Holder

MIX5096 EACH
EUR 190.8

Tube Rack for 7x50ml QuEChERs, horizontal

BV1010-50HQ 1 PC
EUR 42.4

Tube Rack for 7x50mL QuEChERs horizontal

MIX7044 EACH
EUR 57.6

MAGic Clamp™ Tube Rack, 2 x 50ml tubes, horizontal

H1000-MR-T500H 1 PC
EUR 122

Genie Horizontal Orbital Shaker Attachments

MIX5321 EACH
EUR 603.6

maxiplus horizontal unit 26x16-24-32cm

EHS1500-SYS ea
EUR 1132.8

midiplus horizontal unit 15x7-10-15 cm

EHS1300-SYS ea
EUR 496.8

MAGic Clamp Tube Rack 4 x 15mL tubes horizontal

MIX7174 EACH
EUR 163.2

Exploiting one fluorofore per molecule of stoichiometric labeling, detection limit (s / n> 3) and quantitusion limit (s / n> 10) is determined as 0.025 and 0.100 mg / ml, with good linearity. Based on the calibration plot, the amount of low concentration HMO is determined from the ASI sample.

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