The analytic method of electrophor gel electrophoresis (age) has been developed to separate the lignin fraction according to the molecular weight (MW), charge, and shape. The operating conditions to influence species separation have been evaluated along with imaging parameters. Kraft, soda (protobind), and lignin organosolv show different differences in migration. The band is cut, extracted, and analyzed cross with gel permeation chromatography (GPC), 1H NMR, and Pyrolysis GC / MS to confirm their identity as lignin.
The tape intensity correlates with lignin concentration by running a diluted sample serially and imaging each path to produce the right calibration curve. Age techniques are used to monitor and compare enzymatic, bacteria, chemistry, and hydrothermal lignin digestions. Each method shows change in lignin migration and the intensity of the ribbon from time to time. Low MW species are seen in samples collected from the anode buffer tank. Although it requires further development, the age method can provide structural information about lignin and can be accessed by biological and chemical laboratories.
Analysis of West Blotting from proteins separated by Gelose’s original gel electrophoresis
West Blotting was tried to analyze proteins separated by the original gel electrophoresis so that previously developed on the MES system. This report shows a simple protocol to damage the original gel to become a PVDF membrane by soaking gel in the transfer buffer containing sodium dodecylsulfate and 3 examples of analysis. The first example shows the expression of recombinant antibodies in HEK293 cells with direct coloring from the original gel to protein and nucleic acid and script staining for proteins and host cell proteins.
These analyzes indicate the usefulness of the original gel electrophoresis wave, confirming that recombinant antibodies migrated towards the cathode while nucleic acid and the majority of host cell proteins migrated to the anode. The second example shows the state of the phosphorylation of map kinase on the lines of human lymphocytes. That is, the original gel agarose can separate kinases, phosphorylation that can be analyzed by West Bloting. The third example shows the correlation of escherichia coli β-galactosidase expressions between oligomeral activity and enzymes using antibodies and substrate coloring.
Label Electrophoresis gel high sensitivity protein compatible with mass spectrometry
Sodium Dodecyl Sulfate Electrophoresis Gel Poliakrilamide (SDS-Page) is a technique that is widely used for macromolecular and protein analysis. While some methods exist to visualize protein bands separated on gel, one of the most popular coloring methods of protein with coomassie dyes. A newer approach is to use Bio-rad stain technology to visualize protein bands with UV rays and achieve similar or greater sensitivity than Coomassie dyes. Here, we develop a method to further strengthen the sensitivity of the free stain gel using Carboxyfluorescent Succinimidyl Esther (CFSE) coloring.
We compare our novel method using fetal cow serum samples with dye coomassie, standard stain-free gel, and silver coloring. Our results show that while silver coloring remains a gold standard method in terms of sensitivity; Parnisan CFSE samples before use with stain-free gel produce increased sensitivity of 10-100-fold for Coomassie coloring and standard stain standard methods. Our method offers sensitivity similar to silver coloring that is compatible with downstream mass spectrometry, and is therefore more profitable for further recording and macromolecular analysis in the ribbon.
New quantitative gel electrophoresis method with image-based detection for determining food coloring and metallic ions
This work explains the alternative application of electrophoresis gel for separation and quantification of analytes with low molecular weight using innovative and low-cost equipment that allows acquisition of image-based electrofesterogram with webcam. As proof of concepts, determining CU and NI content in metal alloys are evaluated by means of separation and detection of metal ions, which were previously complex with Black T. Eriichrome, Determination of Sunset Yellow FCF food staining material, Tartrazine, brilliant blue FCF and Amaranth Red in refreshing samples Powder investigated as an alternative to a very stable method used for this purpose.
For all investigated analysis, the appropriate electrophoretic peak indicates the signal ratio of noise ranges from 10 to 180, appropriate precision in the area (RSD <3.5%) and linear relationship (R> 0.99) between RGB detected signaled and Concentration of standard solutions. The application of methods for determining CU and NI content in metal alloys provide results without significant differences, at a 95% confidence level, when compared to the results obtained by the FAA-based method. A clear recovery is estimated to sample refresher powder enriched with food coloring ranging from 93% to 108% for the content and content found, indicating the absence of a matrix effect. Studies prove the feasibility of separation and color analytic quantification with gel electrophoresis and image-based detection that can be useful for different samples.
High resolution analysis ultrafast oligosacakaride human milk with multicapillary gel electrophoresis
There is a lot of interest that grows towards the synthesized (HMO) human milk oligosaccharide as a baby formula additive, and is also interesting as a food supplement for adults. At present some producers synthesize HMO, however, their analysis is challenging, both in resolution and speed. In this paper, the ultrafast high resolution method was introduced for HMO separation with multicapillary gel electrophoresis.
Two gel compositions are evaluated with complementary resolution forces. One is a matrix separation of carbohydrate carbohydrate standards of industry used conventionally, completing oligosaccharides according to their costs for hydrodynamic volume ratios. The other is a gel dextran buffer buffer, which utilizes a secondary balance of the complexity of powder-vicinal powder to increase resolution. Considering fast analytical time and multiplexing (12 channel systems), sample plates 96 can be analyzed in less than 80 minutes with conventional type carbohydrate separation matrix and in less than an hour with borat buffer buffer gel.
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs |
ELE2302 |
Scientific Laboratory Supplies |
EACH |
EUR 1681.2 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
4250-050-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
4250-050-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
4250-050-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
CometAssay Electrophoresis System |
4250-050-ES |
Biotechne |
1 Kit |
EUR 3540.96 |
myGel Mini Electrophoresis System |
BM0286 |
GenDepot |
System |
EUR 741.6 |
electrophoresis power supply uk plug |
EV245-UK |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230 |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230-UK |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
EV2230-US |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
EV2320 |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
EV2320-UK |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
EV2320-US |
Consort |
ea |
EUR 898.8 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150 |
Consort |
ea |
EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150-UK |
Consort |
ea |
EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
EV3150-US |
Consort |
ea |
EUR 960 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330 |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330-UK |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
EV3330-US |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
EV3620 |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
EV3620-UK |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
EV3620-US |
Consort |
ea |
EUR 1456.8 |
electrophoresis ps 400v, 500ma, 50w |
EV1450 |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
EV1450-UK |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
EV1450-US |
Consort |
ea |
EUR 574.8 |
electrophoresis ps 300v, 1000ma, 150w |
EV2310 |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
EV2310-UK |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
EV2310-US |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
EV2650 |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
EV2650-UK |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
EV2650-US |
Consort |
ea |
EUR 686.4 |
electrophoresis ps 300v, 2000ma, 300w |
EV3020 |
Consort |
ea |
EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
EV3020-UK |
Consort |
ea |
EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
EV3020-US |
Consort |
ea |
EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610 |
Consort |
ea |
EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610-UK |
Consort |
ea |
EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
EV3610-US |
Consort |
ea |
EUR 960 |
electrophoresis power supply |
EV245 |
Consort |
ea |
EUR 574.8 |
Agarose for DNA electrophoresis |
16-0027-500 |
Medicago |
500 g |
EUR 410.4 |
Gel Electrophoresis System with Time Control, 220-240 V |
Q-EP-2114 |
ABM |
1 |
EUR 1180 |
6X6 well Electrophoresis Apparatus |
EDK626 |
Bio Basic |
1 UNIT |
EUR 750.6 |
|
electrophoresis power supply 120 vac |
EV245-US |
Consort |
ea |
EUR 574.8 |
Gel Electrophoresis System with Muticaster, 100-120 V Input |
Q-EP-2034 |
ABM |
1 |
EUR 1715 |
Gel Electrophoresis System with Muticaster, 220-240 V Input |
Q-EP-2056 |
ABM |
1 |
EUR 2125 |
Gel Electrophoresis System with Time Control, 100-120 V Input |
Q-EP-2100 |
ABM |
1 |
EUR 1385 |
CometAssay Electrophoresis Starter Kit |
4250-050-ESK |
Biotechne |
1 Kit |
EUR 3749.28 |
myGel Mini Electrophoresis System 230V (includes transformer) |
BM0291 |
GenDepot |
System |
EUR 789.6 |
myGel Mini Electrophoresis System 230V (includes transformer) |
E1101-E |
Benchmark Scientific |
1 PC |
EUR 652.5 |
ES Electrophoresis Casting Tray, 7cm X 7cm |
LE1011-A2 |
GenDepot |
Ea |
EUR 181.2 |
ES 6 Tooth Gel Comb For ES Electrophoresis |
LE1011-A4 |
GenDepot |
Ea |
EUR 148.8 |
ES Electrophoresis Casting Tray, 7cm X 14cm |
LE1011-A1 |
GenDepot |
Ea |
EUR 198 |
ES 8/10 Double Gel Comb For ES Electrophoresis |
LE1011-A3 |
GenDepot |
Ea |
EUR 148.8 |
Electrophoresis Apparatus - 7x10cm (6-10-well) |
EDK611 |
Bio Basic |
1 UNIT |
EUR 383.11 |
|
Electrophoresis Apparatus - 7x14cm (6-10-well) |
EDK613 |
Bio Basic |
1 UNIT |
EUR 487.51 |
|
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380 |
ACT-CBMN-7 |
ACTGene |
each |
EUR 892.8 |
CometAssay Single Cell Gel Electrophoresis Assay |
4250-050-K |
Biotechne |
50 tests |
EUR 513.6 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
20840057-1 |
Bio-WORLD |
500 mL |
EUR 74.66 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
20840057-2 |
Bio-WORLD |
1 L |
EUR 139.51 |
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well) |
EDK614 |
Bio Basic |
1 UNIT |
EUR 520.92 |
|
10-well protein Vertical Electrophoresis Apparatus |
EDK692 |
Bio Basic |
1 UNIT |
EUR 487.51 |
|
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator |
E1201 |
Benchmark Scientific |
1 each |
EUR 1001.7 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
21440026-1 |
Bio-WORLD |
15 mL |
EUR 17.3 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
21440026-2 |
Bio-WORLD |
25 mL |
EUR 23.23 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
21440026-3 |
Bio-WORLD |
50 mL |
EUR 35.42 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
21440026-4 |
Bio-WORLD |
100 mL |
EUR 62.99 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100) |
E1101-SK |
Benchmark Scientific |
1 each |
EUR 712.8 |
CometAssay Single Cell Gel Electrophoresis Assay-Silver |
4251-050-K |
Biotechne |
1 kit |
EUR 684.96 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100) |
E1101-SK-E |
Benchmark Scientific |
1 PC |
EUR 742.5 |
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input |
E1201-E |
Benchmark Scientific |
1 each |
EUR 1058.9 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
E1WP3231 |
EnoGene |
50T |
EUR 100 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
E1WP3232 |
EnoGene |
100T |
EUR 150 |
OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules |
VS10WCES |
Scientific Laboratory Supplies |
EACH |
EUR 1434.85 |
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system) |
E1WP326 |
EnoGene |
50T |
EUR 100 |
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system) |
E1WP324 |
EnoGene |
50T |
EUR 60 |
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade) |
E37F007 |
EnoGene |
100-μL |
EUR 226.67 |
Description: Reagents-for-ssDNA-or-RNA-electrophoresis |
SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade) |
E37F001 |
EnoGene |
100-μL |
EUR 226.67 |
Description: Reagents-for-dsDNA-electrophoresis |
Horizontal head, for 2 x 50ml |
BCM1606 |
Bio Basic |
1 pcs, 1 UNIT |
EUR 200.68 |
|
IBI QS710 HORIZONTAL UNIT |
IB51000 |
IBI Scientific |
7CM X 10CM FORMAT |
EUR 509.62 |
IBI MP1015 HORIZONTAL UNIT |
IB53000 |
IBI Scientific |
10CM X 15CM FORMAT |
EUR 675.93 |
IBI HR2025 HORIZONTAL UNIT |
IB56000 |
IBI Scientific |
20CM X 25CM FORMAT |
EUR 877.32 |
IBI HR2525 HORIZONTAL UNIT |
IB57000 |
IBI Scientific |
25CM X 25CM FORMAT |
EUR 965.82 |
mini horizontal unit 7x7 cm |
EHS1101-SYS |
Consort |
ea |
EUR 381.6 |
mini horizontal unit 7x10 cm |
EHS1102-SYS |
Consort |
ea |
EUR 381.6 |
midi horizontal unit 10x7 cm |
EHS1201-SYS |
Consort |
ea |
EUR 422.4 |
mini horizontal unit 9x11 cm |
EHS3200-SYS |
Consort |
ea |
EUR 595.2 |
wide horizontal unit 23.5x14 cm |
EHS3600-SYS |
Consort |
ea |
EUR 930 |
wide horizontal unit 23.5x25 cm |
EHS3610-SYS |
Consort |
ea |
EUR 1030.8 |
midi horizontal unit 10x10 cm |
EHS1202-SYS |
Consort |
ea |
EUR 422.4 |
maxi horizontal unit 20x10 cm |
EHS1401-SYS |
Consort |
ea |
EUR 686.4 |
maxi horizontal unit 20x20 cm |
EHS1402-SYS |
Consort |
ea |
EUR 727.2 |
maxi horizontal unit 20x25 cm |
EHS1403-SYS |
Consort |
ea |
EUR 747.6 |
mini horizontal unit 12x14 cm |
EHS3300-SYS |
Consort |
ea |
EUR 625.2 |
midi horizontal unit 15x15 cm |
EHS3400-SYS |
Consort |
ea |
EUR 757.2 |
midi horizontal unit 15x25 cm |
EHS3410-SYS |
Consort |
ea |
EUR 930 |
midi horizontal unit 20x25 cm |
EHS3500-SYS |
Consort |
ea |
EUR 980.4 |
Horizontal head, for 12 x 1.5ml |
BCM1604 |
Bio Basic |
1 pcs, 1 UNIT |
EUR 200.68 |
|
Horizontal head, for 4 x 15ml |
BCM1605 |
Bio Basic |
1 pcs, 1 UNIT |
EUR 200.68 |
|
midiplus horizontal unit 15x7 cm |
EHS1301-SYS |
Consort |
ea |
EUR 433.2 |
mini horizontal unit 7x7 & 7x10 cm |
EHS1100-SYS |
Consort |
ea |
EUR 422.4 |
midiplus horizontal unit 15x10 cm |
EHS1302-SYS |
Consort |
ea |
EUR 433.2 |
midiplus horizontal unit 15x15 cm |
EHS1303-SYS |
Consort |
ea |
EUR 433.2 |
maxiplus horizontal unit 26x16 cm |
EHS1501-SYS |
Consort |
ea |
EUR 939.6 |
maxiplus horizontal unit 26x24 cm |
EHS1502-SYS |
Consort |
ea |
EUR 939.6 |
maxiplus horizontal unit 26x32 cm |
EHS1503-SYS |
Consort |
ea |
EUR 939.6 |
midi horizontal unit 10x7 & 10x10 cm |
EHS1200-SYS |
Consort |
ea |
EUR 453.6 |
AXYGEN® HORIZONTAL GEL BOX, 10 CM |
HGB-10 |
CORNING |
1/pk |
EUR 399.6 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® HORIZONTAL GEL BOX, 15 CM |
HGB-15 |
CORNING |
1/pk |
EUR 583.2 |
Description: Lab Equipment; Axygen Branded EQ |
AXYGEN® HORIZONTAL GEL BOX, 7 CM |
HGB-7 |
CORNING |
1/pk |
EUR 343.2 |
Description: Lab Equipment; Axygen Branded EQ |
maxi horizontal unit 20x10 & 20x20 cm |
EHS1400-SYS |
Consort |
ea |
EUR 727.2 |
wide horizontal unit 23.5x40 cm ex. port |
EHS3620-SYS |
Consort |
ea |
EUR 1132.8 |
maxiplus horizontal unit 26x16-24-32cm |
EHS1500-SYS |
Consort |
ea |
EUR 1132.8 |
midiplus horizontal unit 15x7-10-15 cm |
EHS1300-SYS |
Consort |
ea |
EUR 496.8 |
Exploiting one fluorofore per molecule of stoichiometric labeling, detection limit (s / n> 3) and quantitusion limit (s / n> 10) is determined as 0.025 and 0.100 mg / ml, with good linearity. Based on the calibration plot, the amount of low concentration HMO is determined from the ASI sample.