The analytic method of electrophor gel electrophoresis (age) has been developed to separate the lignin fraction according to the molecular weight (MW), charge, and shape. The operating conditions to influence species separation have been evaluated along with imaging parameters. Kraft, soda (protobind), and lignin organosolv show different differences in migration. The band is cut, extracted, and analyzed cross with gel permeation chromatography (GPC), 1H NMR, and Pyrolysis GC / MS to confirm their identity as lignin.

The tape intensity correlates with lignin concentration by running a diluted sample serially and imaging each path to produce the right calibration curve. Age techniques are used to monitor and compare enzymatic, bacteria, chemistry, and hydrothermal lignin digestions. Each method shows change in lignin migration and the intensity of the ribbon from time to time. Low MW species are seen in samples collected from the anode buffer tank. Although it requires further development, the age method can provide structural information about lignin and can be accessed by biological and chemical laboratories.

Analysis of West Blotting from proteins separated by Gelose’s original gel electrophoresis

West Blotting was tried to analyze proteins separated by the original gel electrophoresis so that previously developed on the MES system. This report shows a simple protocol to damage the original gel to become a PVDF membrane by soaking gel in the transfer buffer containing sodium dodecylsulfate and 3 examples of analysis. The first example shows the expression of recombinant antibodies in HEK293 cells with direct coloring from the original gel to protein and nucleic acid and script staining for proteins and host cell proteins.

These analyzes indicate the usefulness of the original gel electrophoresis wave, confirming that recombinant antibodies migrated towards the cathode while nucleic acid and the majority of host cell proteins migrated to the anode. The second example shows the state of the phosphorylation of map kinase on the lines of human lymphocytes. That is, the original gel agarose can separate kinases, phosphorylation that can be analyzed by West Bloting. The third example shows the correlation of escherichia coli β-galactosidase expressions between oligomeral activity and enzymes using antibodies and substrate coloring.

Label Electrophoresis gel high sensitivity protein compatible with mass spectrometry

Sodium Dodecyl Sulfate Electrophoresis Gel Poliakrilamide (SDS-Page) is a technique that is widely used for macromolecular and protein analysis. While some methods exist to visualize protein bands separated on gel, one of the most popular coloring methods of protein with coomassie dyes. A newer approach is to use Bio-rad stain technology to visualize protein bands with UV rays and achieve similar or greater sensitivity than Coomassie dyes. Here, we develop a method to further strengthen the sensitivity of the free stain gel using Carboxyfluorescent Succinimidyl Esther (CFSE) coloring.

We compare our novel method using fetal cow serum samples with dye coomassie, standard stain-free gel, and silver coloring. Our results show that while silver coloring remains a gold standard method in terms of sensitivity; Parnisan CFSE samples before use with stain-free gel produce increased sensitivity of 10-100-fold for Coomassie coloring and standard stain standard methods. Our method offers sensitivity similar to silver coloring that is compatible with downstream mass spectrometry, and is therefore more profitable for further recording and macromolecular analysis in the ribbon.

New quantitative gel electrophoresis method with image-based detection for determining food coloring and metallic ions

This work explains the alternative application of electrophoresis gel for separation and quantification of analytes with low molecular weight using innovative and low-cost equipment that allows acquisition of image-based electrofesterogram with webcam. As proof of concepts, determining CU and NI content in metal alloys are evaluated by means of separation and detection of metal ions, which were previously complex with Black T. Eriichrome, Determination of Sunset Yellow FCF food staining material, Tartrazine, brilliant blue FCF and Amaranth Red in refreshing samples Powder investigated as an alternative to a very stable method used for this purpose.

For all investigated analysis, the appropriate electrophoretic peak indicates the signal ratio of noise ranges from 10 to 180, appropriate precision in the area (RSD <3.5%) and linear relationship (R> 0.99) between RGB detected signaled and Concentration of standard solutions. The application of methods for determining CU and NI content in metal alloys provide results without significant differences, at a 95% confidence level, when compared to the results obtained by the FAA-based method. A clear recovery is estimated to sample refresher powder enriched with food coloring ranging from 93% to 108% for the content and content found, indicating the absence of a matrix effect. Studies prove the feasibility of separation and color analytic quantification with gel electrophoresis and image-based detection that can be useful for different samples.

High resolution analysis ultrafast oligosacakaride human milk with multicapillary gel electrophoresis

There is a lot of interest that grows towards the synthesized (HMO) human milk oligosaccharide as a baby formula additive, and is also interesting as a food supplement for adults. At present some producers synthesize HMO, however, their analysis is challenging, both in resolution and speed. In this paper, the ultrafast high resolution method was introduced for HMO separation with multicapillary gel electrophoresis.

Two gel compositions are evaluated with complementary resolution forces. One is a matrix separation of carbohydrate carbohydrate standards of industry used conventionally, completing oligosaccharides according to their costs for hydrodynamic volume ratios. The other is a gel dextran buffer buffer, which utilizes a secondary balance of the complexity of powder-vicinal powder to increase resolution. Considering fast analytical time and multiplexing (12 channel systems), sample plates 96 can be analyzed in less than 80 minutes with conventional type carbohydrate separation matrix and in less than an hour with borat buffer buffer gel.

maxi horizontal unit 20x10 cm
EHS1401-SYS	Consort	ea	EUR 572

maxi horizontal unit 20x20 cm
EHS1402-SYS	Consort	ea	EUR 606

maxi horizontal unit 20x25 cm
EHS1403-SYS	Consort	ea	EUR 623

maxiplus horizontal unit 26x16 cm
EHS1501-SYS	Consort	ea	EUR 783

maxiplus horizontal unit 26x24 cm
EHS1502-SYS	Consort	ea	EUR 783

maxiplus horizontal unit 26x32 cm
EHS1503-SYS	Consort	ea	EUR 783

mini horizontal unit 9x11 cm
EHS3200-SYS	Consort	ea	EUR 496

mini horizontal unit 12x14 cm
EHS3300-SYS	Consort	ea	EUR 521

midi horizontal unit 15x15 cm
EHS3400-SYS	Consort	ea	EUR 631

midi horizontal unit 15x25 cm
EHS3410-SYS	Consort	ea	EUR 775

midi horizontal unit 20x25 cm
EHS3500-SYS	Consort	ea	EUR 817

wide horizontal unit 23.5x14 cm
EHS3600-SYS	Consort	ea	EUR 775

wide horizontal unit 23.5x25 cm
EHS3610-SYS	Consort	ea	EUR 859

mini horizontal unit 7x7 & 7x10 cm
EHS1100-SYS	Consort	ea	EUR 352

midi horizontal unit 10x7 & 10x10 cm
EHS1200-SYS	Consort	ea	EUR 378

maxi horizontal unit 20x10 & 20x20 cm
EHS1400-SYS	Consort	ea	EUR 606

maxiplus horizontal unit 26x16-24-32cm
EHS1500-SYS	Consort	ea	EUR 944

midiplus horizontal unit 15x7-10-15 cm
EHS1300-SYS	Consort	ea	EUR 414

wide horizontal unit 23.5x40 cm ex. port
EHS3620-SYS	Consort	ea	EUR 944

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels
LE1001	GenDepot	Set	EUR 543

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels
LE1002	GenDepot	Set	EUR 583

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels
LE1003	GenDepot	Set	EUR 489

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels
LE1004	GenDepot	Set	EUR 739

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels
LE1005	GenDepot	Set	EUR 901

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels
LE1006	GenDepot	Set	EUR 975

CometAssay Electrophoresis Starter Kit plus EU Power Supply
4250-050-ESK-PS2	Biotechne	1 Kit	EUR 3831.4

CometAssay Electrophoresis Starter Kit plus UK Power Supply
4250-050-ESK-PS3	Biotechne	1 Kit	EUR 3831.4

CometAssay Electrophoresis Starter Kit plus SZ Power Supply
4250-050-ESK-PS4	Biotechne	1 Kit	EUR 3831.4

Horizontal head
BV1000-H15	Benchmark Scientific	1 PC	EUR 134.38

Horizontal head
BV1000-H150	Benchmark Scientific	1 PC	EUR 134.38

Horizontal head
BV1000-H500	Benchmark Scientific	1 PC	EUR 2344.4

Lambda DNA/HindIII Plus, Ready-to-use
M105R-2	Bio Basic	5x100loading, 500prep	EUR 115.25

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)
31020-2	Biotium	50x1mL	EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

2 × Taq Plus Master Mix (Dye Plus)
P212-01	Vazyme	5 ml	EUR 133

2 × Taq Plus Master Mix (Dye Plus)
P212-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix (Dye Plus)
P212-03	Vazyme	50 ml	EUR 389

Horizontal head, for 2 x 50ml
BCM1606	Bio Basic	1 pcs, 1 UNIT	EUR 167.23

electrophoresis power supply
EV245	Consort	ea	EUR 479

CometAssay Electrophoresis System
4250-050-ES	Biotechne	1 Kit	EUR 2950.8

2 × Taq Plus Master Mix II (Dye Plus)
P213-01	Vazyme	5 ml	EUR 134

2 × Taq Plus Master Mix II (Dye Plus)
P213-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix II (Dye Plus)
P213-03	Vazyme	50 ml	EUR 389

Multiple Tumor Tissue Array - 47 different tumors and their corresponding normal tissues, plus 2 control spots
T8235713-2	Biochain	2 slides	EUR 391
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
31014-2	Biotium	50x1mL	EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)
31015-2	Biotium	50x1mL	EUR 3576
Description: Minimum order quantity: 1 unit of 50x1mL

6X6 well Electrophoresis Apparatus
EDK626	Bio Basic	1 UNIT	EUR 625.5

Starch (Smithies), for electrophoresis
GE6857-1KG	Glentham Life Sciences	1 kg	EUR 309

Starch (Smithies), for electrophoresis
GE6857-500G	Glentham Life Sciences	500 g	EUR 181

Acrylamide, suitable for electrophoresis
GK5651-100G	Glentham Life Sciences	100 g	EUR 54

myGel Mini Electrophoresis System
BM0286	GenDepot	System	EUR 618

Agarose for DNA electrophoresis
16-0027-500	Medicago	500 g	EUR 342

CometAssay Electrophoresis Starter Kit
4250-050-ESK	Biotechne	1 Kit	EUR 3124.4

2 × Taq Plus Master Mix
P211-01	Vazyme	5 ml	EUR 134

2 × Taq Plus Master Mix
P211-02	Vazyme	15 ml	EUR 193

2 × Taq Plus Master Mix
P211-03	Vazyme	50 ml	EUR 389

Breast Tumor Tissue Array - 64 Different Breast tumors. Plus positive control and negative control
T8235721-2	Biochain	2 slides	EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Colon Tumor Tissue Array - 64 Different Colon tumors. Plus positive control and negative control
T8235722-2	Biochain	2 slides	EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Rectum Tumor Tissue Array - 64 Different Rectum tumors. Plus positive control and negative control
T8235723-2	Biochain	2 slides	EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Lung Tumor Tissue Array - 64 Different Lung tumors. Plus positive control and negative control
T8235724-2	Biochain	2 slides	EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Ovary Tumor Tissue Array - 64 Different Ovary tumors. Plus positive control and negative control
T8235725-2	Biochain	2 slides	EUR 307
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

100 bp DNA Ladder Plus, loading dye included, 50 ug, 0.5 ug per lane
Z6030001-2	Biochain	100 lanes	EUR 102

SureClean Plus
BIO-37047	Bioline	1 x 5ml	Ask for price

SureClean Plus
BIO-37048	Bioline	2 x 12.5ml	Ask for price

6 x 15ml horizontal rotisserie
R4040-HZ15	Benchmark Scientific	1 PC	EUR 106.47

6 x 50ml horizontal rotisserie
R4040-HZ50	Benchmark Scientific	1 PC	EUR 164.69

electrophoresis ps 400v, 500ma, 50w
EV1450	Consort	ea	EUR 479

electrophoresis ps 400v, 500ma, 50w
EV1450-UK	Consort	ea	EUR 479

electrophoresis ps 400v, 500ma, 50w
EV1450-US	Consort	ea	EUR 479

electrophoresis ps 1500v, 300ma, 150w
EV2230	Consort	ea	EUR 749

electrophoresis ps 1500v, 300ma, 150w
EV2230-UK	Consort	ea	EUR 749

electrophoresis ps 1500v, 300ma, 150w
EV2230-US	Consort	ea	EUR 749

electrophoresis ps 300v, 1000ma, 150w
EV2310	Consort	ea	EUR 572

electrophoresis ps 300v, 1000ma, 150w
EV2310-UK	Consort	ea	EUR 572

electrophoresis ps 300v, 1000ma, 150w
EV2310-US	Consort	ea	EUR 572

electrophoresis ps 3000v, 150ma, 150w
EV2320	Consort	ea	EUR 749

electrophoresis ps 3000v, 150ma, 150w
EV2320-UK	Consort	ea	EUR 749

electrophoresis ps 3000v, 150ma, 150w
EV2320-US	Consort	ea	EUR 749

electrophoresis power supply uk plug
EV245-UK	Consort	ea	EUR 479

electrophoresis power supply 120 vac
EV245-US	Consort	ea	EUR 479

electrophoresis ps 600v, 500ma, 150w
EV2650	Consort	ea	EUR 572

electrophoresis ps 600v, 500ma, 150w
EV2650-UK	Consort	ea	EUR 572

electrophoresis ps 600v, 500ma, 150w
EV2650-US	Consort	ea	EUR 572

electrophoresis ps 300v, 2000ma, 300w
EV3020	Consort	ea	EUR 800

electrophoresis ps 300v, 2000ma, 300w
EV3020-UK	Consort	ea	EUR 800

electrophoresis ps 300v, 2000ma, 300w
EV3020-US	Consort	ea	EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150	Consort	ea	EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150-UK	Consort	ea	EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150-US	Consort	ea	EUR 800

electrophoresis ps 3000v, 300ma, 300w
EV3330	Consort	ea	EUR 1214

electrophoresis ps 3000v, 300ma, 300w
EV3330-UK	Consort	ea	EUR 1214

electrophoresis ps 3000v, 300ma, 300w
EV3330-US	Consort	ea	EUR 1214

electrophoresis ps 600v, 1000ma, 300w
EV3610	Consort	ea	EUR 800

Exploiting one fluorofore per molecule of stoichiometric labeling, detection limit (s / n> 3) and quantitusion limit (s / n> 10) is determined as 0.025 and 0.100 mg / ml, with good linearity. Based on the calibration plot, the amount of low concentration HMO is determined from the ASI sample.