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Two-dimensional gel electrophoresis (2D-GE) image analysis based on CellProfiler: Pseudomonas aeruginosa AG1 as model

Two-dimensional gel electrophoresis (2D-GE) image analysis based on CellProfiler: Pseudomonas aeruginosa AG1 as model
Posted by Troy

The two-dimensional gel electrophoresis (2D-GE) is a technique that is very necessary for the study of biological system proteomas, providing an assessment of changes in abundance of proteins in various experimental conditions. However, because of the complexity of 2D-GE gel, there is no systematic, automatic, and reproducing protocol for image analysis and specific implementation is needed for each context. In addition, practically all available solutions are commercial, which implies high costs and a little flexibility to modulate algorithm parameters.

Using bacterial strains, pseudomonas aeruginosaag1 as a model, we get pictures of 2D-GE protein protein profiles periplasm when strains are exposed to several conditions, including antibiotics. Then, we proceed to implement and evaluate the image analysis protocol with open source software, CellProfiler. First, step preprocessing includes bunwarpj-image pipes to align 2D-GE images. Then, using CellProfiler, we standed two pipes for place identification. The total introduction of places is achieved by using segmentation with intensity, which performance is evaluated when compared to the reference protocol.

In the second pipe with the same program, the identification of differential spots is handled when comparing pairs of protein profiles. Because the program characteristics used, our workflow can automatically analyze a large number of images and parallel, which is an advantage in connection with other implementations. Finally, we compare six experimental conditions of bacterial pressure in the presence or absence of antibiotics, determine the relationship of protein profiles by applying the PCA clustering algorithm (main component analysis) and hierarchy (clustering).

Electrophoresis gel polyacrylamide

Chains of cross-linked from polyacrylamides can be used as electric neutral gel to separate multiple-double DNA fragments according to the size and DNAS stranded single according to size and conformation. Polyacrylamide gel has three main advantages along with axible glass: (1) the power of resolution is so large that they can separate the DNA molecules that are different in length to only 0.1% (eg) 1000 bp). (2) They can accommodate the amount of DNA which is far greater than agarose gel. Up to 10 μg of DNA can be applied to one slot (1 cm × 1 mm) from a typical polyacrylamide gel without losing resolution. (3) DNA recovers from polyacrylamide gel is very pure and can be used for the most demanding purpose (eg, microinjection embryo mouse).

However, polyacrylamide gel has losses to be more difficult to be prepared and handled than gel agarose. The method is presented here to prepare and run polyacrylamide gels that are not prominent and for DNA detection on this gel with coloring.

Comparative analysis using electrophoresis of pulsating gel-field highlights the transmission of salmonella potential between buffalo and asimptomatic pigs on one agriculture

Buffalo and pigs play an important epidemiological roll in the cycle of salmonella infection, and asymptomatic animals can act as a key component in the spread of this disease with horizontal, vertical, and cross species transmissions. Our research is intended and able to confirm the evidence of transmission of cross-agona salmonella species between buffalo and asimptomatic pigs. Also, we describe salmonella infection in the pork production phase, which involves Serotype Agona, Senftenberg and Schwarzengrund.

 Two-dimensional gel electrophoresis (2D-GE) image analysis based on CellProfiler: Pseudomonas aeruginosa AG1 as model

Rectal samples are collected from buffalo jafarabadi (n = 25) and pig piau (n = 32), located on an agriculture. Salmonella Agona was isolated from the buffalo of breastfeeding, gilt, sowing pregnant, and weaning pigs, Salmonella Schwarzengrund from sowing breastfeeding and Salmonella Senftenberg from Gilt, sowing pregnant, sowing weaning, and weaning pigs. The pulsed gel electrophoresis protocol (PFGE) is done and reveals four different profiles. Profile 1 (Salmonella Agona), isolated from pregnant pigs, gold and two breastfeeding buffalo, revealing the PFGE pattern that cannot be distinguished, confirming proof of cross-species transmission potential. Profile 2 (Salmonella Agona), 3 (Salmonella Senftenberg), and 4 (Salmonella Schwarzengrund), isolated from pigs, revealing pfge patterns that cannot be distinguished, proving salmonella infection in the pork production phase.

Consider the epidemiological relevance of the buffalo and pigs in the cycle of salmonella infection, transmission confirmation of the potential species of salmonella agona and potential salmonella infections in the pork production phase highlight the importance of the formation of preventive health strategies correctly on animal husbandry, in particular the importance of avoiding contact between buffalo and pigs, due to cross transmission Species can occur, increasing the risk of disseminating disease.

Seabeam wild or farming gilead (Sparus Aurata)? How to distinguish between them with two-dimensional gel electrophoresis

Because the shares of world wild fish are limited and market demand increases, fish farming has become an alternative food source and a way to reduce costs for consumers. Agricultural sales as wild fish are fraud practices, because it is important to work to provide new tools and alternatives that can help in combating fraud to protect consumers and to ensure food tracing. Proteomic profiles of farm fish and wildly different from each other. With this study we want to identify the marker of the liver protein through two-dimensional electrophoresis (2DE) which will enable us to distinguish wildly from the Seabream Gilhead farming.

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 181

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 54

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 342

Horizontal head

BV1000-H15 1 PC
EUR 134.38

Horizontal head

BV1000-H150 1 PC
EUR 134.38

Horizontal head

BV1000-H500 1 PC
EUR 2344.4

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 167.23

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 167.23

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 167.23

electrophoresis power supply

EV245 ea
EUR 479

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 2950.8

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 124

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 625.5

myGel Mini Electrophoresis System

BM0286 System
EUR 618

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3124.4

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 124

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 318

mini horizontal unit 7x10 cm

EHS1102-SYS ea
EUR 318

midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 352

midi horizontal unit 10x10 cm

EHS1202-SYS ea
EUR 352

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 361

midiplus horizontal unit 15x10 cm

EHS1302-SYS ea
EUR 361

midiplus horizontal unit 15x15 cm

EHS1303-SYS ea
EUR 361

maxi horizontal unit 20x10 cm

EHS1401-SYS ea
EUR 572

maxi horizontal unit 20x20 cm

EHS1402-SYS ea
EUR 606

maxi horizontal unit 20x25 cm

EHS1403-SYS ea
EUR 623

maxiplus horizontal unit 26x16 cm

EHS1501-SYS ea
EUR 783

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
EUR 783

maxiplus horizontal unit 26x32 cm

EHS1503-SYS ea
EUR 783

mini horizontal unit 9x11 cm

EHS3200-SYS ea
EUR 496

mini horizontal unit 12x14 cm

EHS3300-SYS ea
EUR 521

midi horizontal unit 15x15 cm

EHS3400-SYS ea
EUR 631

midi horizontal unit 15x25 cm

EHS3410-SYS ea
EUR 775

midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 817

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
EUR 775

wide horizontal unit 23.5x25 cm

EHS3610-SYS ea
EUR 859

6 x 15ml horizontal rotisserie

R4040-HZ15 1 PC
EUR 106.47

6 x 50ml horizontal rotisserie

R4040-HZ50 1 PC
EUR 164.69

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 479

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 749

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 572

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 749

electrophoresis power supply uk plug

EV245-UK ea
EUR 479

electrophoresis power supply 120 vac

EV245-US ea
EUR 479

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 572

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 572

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 572

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 800

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1214

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 800

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1214

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
EUR 352

midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 378

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 606

maxiplus horizontal unit 26x16-24-32cm

EHS1500-SYS ea
EUR 944

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 319.26

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 406.26

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 406.26

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 428

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 333
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 486
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 20 CM

HGB-20 1/pk
EUR 541
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 286
Description: Lab Equipment; Axygen Branded EQ

midiplus horizontal unit 15x7-10-15 cm

EHS1300-SYS ea
EUR 414

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS ea
EUR 944

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange 1 prep
EUR 158

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 165

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 151

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 658

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 570.8

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 495.65

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 434.1

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 3831.4

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E

E1101-SK-E 1 PC
EUR 535.53

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7
EUR 744

Adaptor for flexible plates, accessory for AgileSealer?

APS-200-FP
EUR 351

Sample comb for batching for AE-6110

2398313 unit
EUR 100
Description: (90mm・r efer ence at t ached・1.5mm t i ck ness)

gelcaster for sequencer

ESEQ1000-CAST ea
EUR 437

Antibody for Phosphoserine

SPC-149F 0.4ml
EUR 362
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is unconjugated.

Antibody for Phosphoserine

SPC-149F-A390 0.4ml
EUR 409
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 390.

Antibody for Phosphoserine

SPC-149F-A488 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 488.

Antibody for Phosphoserine

SPC-149F-A565 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 565.

Antibody for Phosphoserine

SPC-149F-A594 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 594.

Antibody for Phosphoserine

SPC-149F-A633 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 633.

Antibody for Phosphoserine

SPC-149F-A655 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 655.

Antibody for Phosphoserine

SPC-149F-A680 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 680.

Antibody for Phosphoserine

SPC-149F-A700 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to ATTO 700.

Antibody for Phosphoserine

SPC-149F-ALP 0.4ml
EUR 403
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Alkaline Phosphatase.

Antibody for Phosphoserine

SPC-149F-APC 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to APC .

Antibody for Phosphoserine

SPC-149F-APCCY7 0.4ml
EUR 480
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to APC/Cy7.

Antibody for Phosphoserine

SPC-149F-BI 0.4ml
EUR 405
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Biotin.

Antibody for Phosphoserine

SPC-149F-DY350 0.4ml
EUR 423
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Dylight 350.

Antibody for Phosphoserine

SPC-149F-DY405 0.4ml
EUR 411
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Dylight 405.

Antibody for Phosphoserine

SPC-149F-DY488 0.4ml
EUR 402
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Dylight 488.

Antibody for Phosphoserine

SPC-149F-DY594 0.4ml
EUR 404
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Dylight 594.

Antibody for Phosphoserine

SPC-149F-DY633 0.4ml
EUR 399
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to Dylight 633.

Antibody for Phosphoserine

SPC-149F-FITC 0.4ml
EUR 401
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to FITC.

Antibody for Phosphoserine

SPC-149F-P594 0.4ml
EUR 415
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to PE/ATTO 594.

Antibody for Phosphoserine

SPC-149F-PCP 0.4ml
EUR 408
Description: A polyclonal antibody for Phosphoserine from Species Independent. The antibody is produced in rabbit after immunization with Phosphoserine conjugated to KLH, and phosvitin mixture. The Antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100). This Phosphoserine antibody is conjugated to PerCP.

Heart samples of 32 Gilead Seabream, wild and farming, stored at -80 ° C Previous extraction of protein. Samples are subject to 2DE to detect qualitative and quantitative differences. Proteom analysis shows a protein point (molecular weight ~ 34 kDa and isoelectric point ~ 6.9) only in the sample of the Seabread Wild Gilead; Liquid chromatography Tandem mass spectrometry identifies the place as ubiquitin. Ubiquitin can be a valid marker to distinguish wildly from the Seabread of Gilead farming, thus ensuring sustainable monitoring throughout the commercial chain and fighting commercial fraud.

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