A recent study found that the method of agarose gel electrophoresis (AGE) produced two great bands that differ according to the isoenzyme ALP liver and bone (ALP2 and ALP3, respec-tively) in the serum of bovine treated with protease and neuraminidase (PN-treatment), although there fears that the intestinal ALP isoenzymes (ALP5) often overlap with ALP3 in human serum treated with neuraminidase. Because ALP5 separated from ALP3 in serum, bovine treated with protease alone (P-treatment), we use the method is modified using both P and PN-treated cattle sera to measure the activity of three isoenzymes of ALP in 53 cows Lacta-ting Holstein: 24 primiparous and 29 multipara.
After electrophoresis, 51 of 53 samples (96.2%) had a P-treatment produces a fraction that differ according to the ALP5, as did the control serum. All sera PN-treated produce ALP2 definite fraction. ALP3 fraction is calculated as the remainder after excluding ALP2 and ALP5. Total ALP activity (t-ALP) and ALP3 in primiparous cows higher than those in multiparous cows (p ⟨0,001) in the early-to-peak [10-110 days in milk (DIM)] and mid (111-220 DIM) lactation.
In a multi-parous cows, ALP3 activity in late lactation (221-477 DIM) was significantly higher than in the early-to-peak lactation. Thus, AGE-modified method described here is capable of discrimi-nate the three factions of the ALP isoenzymes in sera lactating cows. ALP isoenzyme patterns circu AGE-Lating will contribute to understanding the physiological status of bone metabolism in lactating cows. Vaccines against infectious diseases is needed.
Therefore, the development of modern analytical methods must be as efficient as possible to accelerate the development of vaccines. The purpose of this study was to identify the critical parameter method (CMPS) and to establish a set of measures to efficiently develop and validate the CE-SDS method for protein analysis based buffer gel vaccine commercially available. The CMPS gained from reviewing the literature and tested the effects of dilution buffer gel.
Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Comparison of PFGE and confirms typing-based whole genome sequencing accuracy of pulsed-field gel electrophoresis to investigate an outbreak of Pseudomonas aeruginosa local
Objective: To determine whether pulsed-field gel electrophoresis (PFGE) accurately recognizes isolates belonging to the cluster is defined by a technique based on whole genome sequencing (WGS) using Pseudomonas aeruginosa as a model.
Methods: We selected 65 ST395 isolates of P. aeruginosa was isolated in seven European hospitals between 1998 and 2012. The isolates typed by PFGE and sorted by WGS. An analysis of the genome core multi-locus sequence typing (cgMLST) by 3831 genes carried by a homemade pipe. Findings: PFGE identified eight pulsotypes and cgMLST distinguished nine and nine single cluster. cluster of five cgMLST and pulsotypes (31/65 isolates) coincide perfectly.
Isolates without apparent epidemiological links grouped by PFGE separated by cgMLST (16/65 isolates) distinguishes the city, indicates that PFGE should be saved to a local outbreak investigation. Importantly, they share the isolates hypermutator pulsotype with their parents (16/65 isolates), while they are not recognized by cgMLST. This suggests that PFGE is less affected than typing based WGS by accelerated genetic drift occurs in epidemic P. aeruginosa.
Agarose Gel Electrophoresis Kit
MB570-10PR
EWC Diagnostics
1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit
20X DoubleQuick DNA Electrophoresis Buffer
73530
Sisco Laboratories
250 ml
EUR 35.58
Description: Part E
20X DoubleQuick DNA Electrophoresis Buffer
73530-1
Sisco Laboratories
500 ml
EUR 64.4
Description: Part E
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-1
Bio-WORLD
15 mL
EUR 21.21
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-2
Bio-WORLD
25 mL
EUR 28.49
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-3
Bio-WORLD
50 mL
EUR 43.44
Protein Antioxidant for SDS-PAGE Gel Electrophoresis
21440026-4
Bio-WORLD
100 mL
EUR 77.25
Agarose Gel Electrophoresis Kit (Teaching)
66602
Sisco Laboratories
15 expt. Kit
EUR 44.05
Description: Part E
ES Horizontal Electrophoresis Set
LE1011
GenDepot
Set
EUR 410
CometAssay Single Cell Gel Electrophoresis Assay
4250-050-K
Biotechne
50 tests
EUR 513.6
HiPer® Agarose Gel Electrophoresis Teach
HTBM001-10PR
EWC Diagnostics
1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach
Glycine for electrophoresis, 99.5%
52574
Sisco Laboratories
100 Gms
EUR 9.58
Description: Part A
Agarose for DNA electrophoresis
16-0027-500
Medicago
500 g
EUR 410.4
Electrophoresis Module for Eco-Mini, for 2 gels
AJ846-017-175
Westburg
each
EUR 604.95
Electrophoresis Module for Eco-Maxi, for 2 gels
AJ846-017-475
Westburg
each
EUR 997.35
CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K
Biotechne
1 kit
EUR 684.96
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380
ACT-CBMN-7
ACTGene
each
EUR 892.8
Aminoacetic acid, ?For electrophoresis
GRM2233-100G
EWC Diagnostics
1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis
Aminoacetic acid, ?For electrophoresis
GRM2233-500G
EWC Diagnostics
1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis
ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2
GenDepot
Ea
EUR 90
Agarose, medium EEO ?For electrophoresis
RM6249-100G
EWC Diagnostics
1 unit
EUR 119.06
Description: Agarose, medium EEO ?For electrophoresis
Agarose, medium EEO ?For electrophoresis
RM6249-10G
EWC Diagnostics
1 unit
EUR 19.63
Description: Agarose, medium EEO ?For electrophoresis
Agarose, medium EEO ?For electrophoresis
RM6249-1KG
EWC Diagnostics
1 unit
EUR 1089.43
Description: Agarose, medium EEO ?For electrophoresis
Agarose, medium EEO ?For electrophoresis
RM6249-25G
EWC Diagnostics
1 unit
EUR 36.87
Description: Agarose, medium EEO ?For electrophoresis
ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1
GenDepot
Ea
EUR 100
Gel Electrophoresis System with Time Control, 220-240 V
Q-EP-2114
ABM
1
EUR 1180
Gel Electrophoresis System with Muticaster, 100-120 V Input
Q-EP-2034
ABM
1
EUR 1715
Gel Electrophoresis System with Muticaster, 220-240 V Input
Q-EP-2056
ABM
1
EUR 2125
Gel Electrophoresis System with Time Control, 100-120 V Input
Q-EP-2100
ABM
1
EUR 1385
Ammonium Persulphate (APS) for electrophoresis, 99%
28575
Sisco Laboratories
25 Gms
EUR 1.51
Description: Part A
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-005mL
MyBiosource
0.05mL
EUR 195
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-01mL
MyBiosource
0.1mL
EUR 255
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-5x005mL
MyBiosource
5x0.05mL
EUR 820
SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579833-5x01mL
MyBiosource
5x0.1mL
EUR 1100
Tris-HEPES-SDS Buffer 20X for Electrophoresis
20840057-1
Bio-WORLD
500 mL
EUR 91.56
Tris-HEPES-SDS Buffer 20X for Electrophoresis
20840057-2
Bio-WORLD
1 L
EUR 173.27
electrophoresis ps 1500v, 300ma, 150w
EV2230
Consort
ea
EUR 898.8
electrophoresis ps 1500v, 300ma, 150w
EV2230-UK
Consort
ea
EUR 898.8
electrophoresis ps 1500v, 300ma, 150w
EV2230-US
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320-UK
Consort
ea
EUR 898.8
electrophoresis ps 3000v, 150ma, 150w
EV2320-US
Consort
ea
EUR 898.8
ELECTROPHORESIS PS 1200V, 500MA, 300W
EV3150
Consort
ea
EUR 1060
ELECTROPHORESIS PS 1200V, 500MA, 300W
EV3150-UK
Consort
ea
EUR 1060
ELECTROPHORESIS PS 3000V, 200MA, 300W
EV3330
Consort
ea
EUR 1600
ELECTROPHORESIS PS 3000V, 200MA, 300W
EV3330-UK
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W
EV3620
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W
EV3620-UK
Consort
ea
EUR 1600
ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels
LE1003
GenDepot
Set
EUR 410
CometAssay Electrophoresis System
4250-050-ES
Biotechne
1 Kit
EUR 3540.96
Electrophoresis Buffer (10x)
MBS2557002-100mL
MyBiosource
100mL
EUR 85
Electrophoresis Buffer (10x)
MBS2557002-200mL
MyBiosource
200mL
EUR 90
Electrophoresis Buffer (10x)
MBS2557002-500mL
MyBiosource
500mL
EUR 95
Electrophoresis Buffer (10x)
MBS2557002-5x500mL
MyBiosource
5x500mL
EUR 380
electrophoresis ps 400v, 500ma, 50w
EV1450
Consort
ea
EUR 574.8
electrophoresis ps 400v, 500ma, 50w
EV1450-UK
Consort
ea
EUR 574.8
electrophoresis ps 400v, 500ma, 50w
EV1450-US
Consort
ea
EUR 574.8
ELECTROPHORESIS PS 300V, 1000MA, 150W
EV2310
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 1000MA, 150W
EV2310-UK
Consort
ea
EUR 750
ELECTROPHORESIS PS 600V, 500MA, 150W
EV2650
Consort
ea
EUR 750
ELECTROPHORESIS PS 600V, 500MA, 150W
EV2650-UK
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 2000MA, 300W
EV3020
Consort
ea
EUR 1060
ELECTROPHORESIS PS 300V, 2000MA, 300W
EV3020-UK
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W
EV3610
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W
EV3610-UK
Consort
ea
EUR 1060
TEMED, electrophoresis grade
RM10358-100G
EWC Diagnostics
1 unit
EUR 301.18
Description: TEMED, electrophoresis grade
electrophoresis power supply
EV245
Consort
ea
EUR 574.8
Electrophoresis Power Supply
LA690-1NO
EWC Diagnostics
1 unit
EUR 432.84
Description: Electrophoresis Power Supply
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-005mL
MyBiosource
0.05mL
EUR 180
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-01mL
MyBiosource
0.1mL
EUR 230
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-05mL
MyBiosource
0.5mL
EUR 695
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-5x005mL
MyBiosource
5x0.05mL
EUR 760
SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)
MBS8579830-5x01mL
MyBiosource
5x0.1mL
EUR 980
SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F007-100-uL
EnoGene
100 μL
EUR 227
Description: Reagents for ssDNA or RNA electrophoresis
SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F007-50-uL
EnoGene
50 μL
EUR 133
Description: Reagents for ssDNA or RNA electrophoresis
Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611
Bio Basic
1 UNIT
EUR 383.11
Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613
Bio Basic
1 UNIT
EUR 487.51
CometAssay Electrophoresis Starter Kit
4250-050-ESK
Biotechne
1 Kit
EUR 3749.28
ELECTROPHORESIS PS 1200V, 500MA, 300W 120 VAC
EV3150-US
Consort
ea
EUR 1060
ELECTROPHORESIS PS 3000V, 200MA, 300W 120 VAC
EV3330-US
Consort
ea
EUR 1600
ELECTROPHORESIS PS 6000V, 100MA, 300W 120 VAC
EV3620-US
Consort
ea
EUR 1600
10X MOPS Electrophoresis Buffer
ML050-500ML
EWC Diagnostics
1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer
ELECTROPHORESIS PS 600V, 500MA, 150W 120VAC
EV2650-US
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 1000MA, 150W 120 VAC
EV2310-US
Consort
ea
EUR 750
ELECTROPHORESIS PS 300V, 2000MA, 300W 120 VAC
EV3020-US
Consort
ea
EUR 1060
ELECTROPHORESIS PS 600V, 1000MA, 300W 120 VAC
EV3610-US
Consort
ea
EUR 1060
SDS-PAGE Electrophoresis Buffer
MBS355536-1L
MyBiosource
1L
EUR 165
SDS-PAGE Electrophoresis Buffer
MBS355536-5x1L
MyBiosource
5x1L
EUR 480
SDS-PAGE Electrophoresis Buffer
MBS355542-10x1L
MyBiosource
10x1L
EUR 250
6X6 well Electrophoresis Apparatus
EDK626
Bio Basic
1 UNIT
EUR 750.6
electrophoresis power supply 120 vac
EV245-US
Consort
ea
EUR 574.8
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-100-uL
EnoGene
100 μL
EUR 227
Description: Reagents for dsDNA electrophoresis
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-500-uL
EnoGene
500 μL
EUR 983
Description: Reagents for dsDNA electrophoresis
SUPER Green I nucleic acid gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
E37F001-50uL
EnoGene
50 μL
EUR 133
Description: Reagents for dsDNA electrophoresis
10-well protein Vertical Electrophoresis Apparatus
EDK692
Bio Basic
1 UNIT
EUR 487.51
electrophoresis power supply uk plug
EV245-UK
Consort
ea
EUR 574.8
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)
EDK614
Bio Basic
1 UNIT
EUR 520.92
ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels
LE1001
GenDepot
Set
EUR 450
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3231
EnoGene
50T
EUR 100
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3232
EnoGene
100T
EUR 150
myGel Mini Electrophoresis System, 115V
BM0286
GenDepot
System
EUR 560
ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels
LE1002
GenDepot
Set
EUR 470
ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels
LE1004
GenDepot
Set
EUR 610
ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels
LE1005
GenDepot
Set
EUR 710
ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels
LE1006
GenDepot
Set
EUR 750
Alkaline Hemoglobin Electrophoresis Kit
MBP001-112RUNS
EWC Diagnostics
1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Hemoglobin Electrophoresis Kit
MBP001-224RUNS
EWC Diagnostics
1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Hemoglobin Electrophoresis Kit
MBP008-224RUNS
EWC Diagnostics
1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit
Alkaline Paper Electrophoresis Kit (Cell
MBP009-20RUNS
EWC Diagnostics
1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell
WEE VERT PROTEIN ELECTROPHORESIS SYSTEM
LA1070-1NO
EWC Diagnostics
1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM
Electrophoresis Power Supply (2 termina
LA842-1NO
EWC Diagnostics
1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina
Cellulose Acetate Electrophoresis System
LA1027-1NO
EWC Diagnostics
1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)
E1WP326
EnoGene
50T
EUR 100
Hi-Eco Mini Horizontal Electrophoresis K
LA851-1NO
EWC Diagnostics
1 unit
EUR 205.56
Description: Hi-Eco Mini Horizontal Electrophoresis K
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)
E1WP324
EnoGene
50T
EUR 60
CometAssay Electrophoresis Starter Kit plus EU Power Supply
4250-050-ESK-PS2
Biotechne
1 Kit
EUR 4597.68
CometAssay Electrophoresis Starter Kit plus UK Power Supply
4250-050-ESK-PS3
Biotechne
1 Kit
EUR 4597.68
CometAssay Electrophoresis Starter Kit plus SZ Power Supply
4250-050-ESK-PS4
Biotechne
1 Kit
EUR 4597.68
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)
E37F007
EnoGene
100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis
myGel Mini Electrophoresis System 230V (includes transformer)
BM0291
GenDepot
System
EUR 580
myGel Mini Electrophoresis System 230V (includes transformer)
E1101-E
Benchmark Scientific
1 PC
EUR 665.55
SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)
E37F001
EnoGene
100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator
E1201
Benchmark Scientific
1 each
EUR 1001.7
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input
E1201-E
Benchmark Scientific
1 each
EUR 1058.9
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)
E1101-SK
Benchmark Scientific
1 each
EUR 727.06
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)
E1101-SK-E
Benchmark Scientific
1 PC
EUR 757.35
OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH
VS10WCES
Scientific Laboratory Supplies
EACH
EUR 1614.21
Electrophoretic Tissue Clearing Solution (12x1L)
LBC13001
Westburg
each
EUR 741.2
Buffer tank E for Eco-Mini (no lid, for electropho
AJ846-017-169
Westburg
each
EUR 322.64
double silver electrode, s7
SP05X
Consort
ea
EUR 279.6
DOUBLE PLATINUM ELECTRODE, S7
SP04X
Consort
ea
EUR 270
DOUBLE SILVER ELECTRODE, PEEK, S7
SP06X
Consort
each
EUR 310
Conclusion: Although the WGS-based typing has logically become the new reference standard, we show here that the PFGE can be used with confidence to local outbreak investigations caused by P. aeruginosa.