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Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation

Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Posted by Troy

A recent study found that the method of agarose gel electrophoresis (AGE) produced two great bands that differ according to the isoenzyme ALP liver and bone (ALP2 and ALP3, respec-tively) in the serum of bovine treated with protease and neuraminidase (PN-treatment), although there fears that the intestinal ALP isoenzymes (ALP5) often overlap with ALP3 in human serum treated with neuraminidase. Because ALP5 separated from ALP3 in serum, bovine treated with protease alone (P-treatment), we use the method is modified using both P and PN-treated cattle sera to measure the activity of three isoenzymes of ALP in 53 cows Lacta-ting Holstein: 24 primiparous and 29 multipara.

After electrophoresis, 51 of 53 samples (96.2%) had a P-treatment produces a fraction that differ according to the ALP5, as did the control serum. All sera PN-treated produce ALP2 definite fraction. ALP3 fraction is calculated as the remainder after excluding ALP2 and ALP5. Total ALP activity (t-ALP) and ALP3 in primiparous cows higher than those in multiparous cows (p ⟨0,001) in the early-to-peak [10-110 days in milk (DIM)] and mid (111-220 DIM) lactation.

In a multi-parous cows, ALP3 activity in late lactation (221-477 DIM) was significantly higher than in the early-to-peak lactation. Thus, AGE-modified method described here is capable of discrimi-nate the three factions of the ALP isoenzymes in sera lactating cows. ALP isoenzyme patterns circu AGE-Lating will contribute to understanding the physiological status of bone metabolism in lactating cows. Vaccines against infectious diseases is needed.

Therefore, the development of modern analytical methods must be as efficient as possible to accelerate the development of vaccines. The purpose of this study was to identify the critical parameter method (CMPS) and to establish a set of measures to efficiently develop and validate the CE-SDS method for protein analysis based buffer gel vaccine commercially available. The CMPS gained from reviewing the literature and tested the effects of dilution buffer gel.

Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation

Comparison of PFGE and confirms typing-based whole genome sequencing accuracy of pulsed-field gel electrophoresis to investigate an outbreak of Pseudomonas aeruginosa local

Objective: To determine whether pulsed-field gel electrophoresis (PFGE) accurately recognizes isolates belonging to the cluster is defined by a technique based on whole genome sequencing (WGS) using Pseudomonas aeruginosa as a model.


Methods: We selected 65 ST395 isolates of P. aeruginosa was isolated in seven European hospitals between 1998 and 2012. The isolates typed by PFGE and sorted by WGS. An analysis of the genome core multi-locus sequence typing (cgMLST) by 3831 genes carried by a homemade pipe.
Findings: PFGE identified eight pulsotypes and cgMLST distinguished nine and nine single cluster. cluster of five cgMLST and pulsotypes (31/65 isolates) coincide perfectly.

Isolates without apparent epidemiological links grouped by PFGE separated by cgMLST (16/65 isolates) distinguishes the city, indicates that PFGE should be saved to a local outbreak investigation. Importantly, they share the isolates hypermutator pulsotype with their parents (16/65 isolates), while they are not recognized by cgMLST. This suggests that PFGE is less affected than typing based WGS by accelerated genetic drift occurs in epidemic P. aeruginosa.

AXYGEN® 8-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.0 MM THICKNESS

HGB15-8-1 1/pk
EUR 82.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-8-15 1/pk
EUR 82.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 7 CM GEL BOX, 0.75 MM THICKNESS

HGB7-8-075 1/pk
EUR 64.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 8-WELL COMB FOR USE WITH 7 CM GEL BOX, 1.0 MM THICKNESS

HGB7-8-1 1/pk
EUR 64.8
Description: Lab Equipment; Axygen Branded EQ

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-10 Ea
EUR 157.2

50 Well Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-10 Ea
EUR 165.6

10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
EUR 157.2

10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-02 Ea
EUR 157.2

10 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-01 Ea
EUR 148.8

10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-02 Ea
EUR 148.8

8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
EUR 153.6

8 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-17 Ea
EUR 157.2

8 Well Comb , 0.75mm Thick For Enduro 7cm Gel System

LE1000C7-08 Ea
EUR 148.8

8 Well Comb ,1mm Thick For Enduro 7cm Gel System

LE1000C7-09 Ea
EUR 148.8

AXYGEN® 10-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.0 MM THICKNESS

HGB15-10-1 1/pk
EUR 72
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 10-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-10-15 1/pk
EUR 72
Description: Lab Equipment; Axygen Branded EQ

AXYGEN®10-WELL COMB FOR USE WITH 7 CM GEL BOX, 1.0 MM THICKNESS

HGB7-10-1 1/pk
EUR 64.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 10-WELL COMB FOR USE WITH 7 CM GEL BOX, 1.5 MM THICKNESS

HGB7-10-15 1/pk
EUR 64.8
Description: Lab Equipment; Axygen Branded EQ

20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-10 Ea
EUR 153.6

AXYGEN® GEL TRAY 10 X 10 CM FOR USE WITH 10 CM GEL BOX, UV TRANSPARENT

HGB10-10-GT 1/pk
EUR 88.8
Description: Lab Equipment; Axygen Branded EQ

10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-01 Ea
EUR 153.6

10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-02 Ea
EUR 153.6

Comb S

2322118 5unit
EUR 308.4
Description: 2pcs/1set

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1448.94

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

Gel Casting S for WSE-1710

2322107 4unit
EUR 339.6
Description: Gel Tr ay (S) x4, Gel Cast i ng St and (S), S si zecomb (9wel l /5wel l ) x2

Gel tray S

2322117 5unit
EUR 308.4
Description: 2pcs/1set

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

ES 936

B5514-10 10 mg
EUR 340.8

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1520.76

AXYGEN® GEL TRAY 15 X 10 CM FOR USE WITH 15 CM GEL BOX, UV TRANSPARENT

HGB15-10-GT 1/pk
EUR 94.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® GEL TRAY 7 X 10 CM FOR USE WITH 7 CM GEL BOX, UV TRANSPARENT

HGB7-10-GT 1/pk
EUR 81.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® AXYPET® PRO 0.5-10 ΜL, 8 CHANNEL PIPETTOR, AUTOCLAVABLE

AP-8-10-P 1/pk
EUR 546
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Gel casting stand S

2322116 5unit
EUR 308.4
Description: 1pc

5 gel caster for 8 x 10cm or 10 x 10cm gels.

CSL6CAST EACH
EUR 508.44

AXYGEN® 10-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-10MC-1 1/pk
EUR 67.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 10-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 10 CM GEL BOX, 1.5 MM THICKNESS

HGB10-10MC-15 1/pk
EUR 67.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 12-WELL COMB FOR USE WITH 10 CM GEL BOX, 0.75 MM THICKNESS

HGB10-12-075 1/pk
EUR 67.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 12-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-12-1 1/pk
EUR 68.4
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 12-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.5 MM THICKNESS

HGB10-12-15 1/pk
EUR 69.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 16-WELL COMB FOR USE WITH 10 CM GEL BOX, 0.75 MM THICKNESS

HGB10-16-075 1/pk
EUR 67.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 16-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-16-1 1/pk
EUR 68.4
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 16-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.5 MM THICKNESS

HGB10-16-15 1/pk
EUR 69.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 16-WELL COMB FOR USE WITH 10 CM GEL BOX, 2.0 MM THICKNESS

HGB10-16-2 1/pk
EUR 70.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-25-1 1/pk
EUR 70.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 25-WELL COMB FOR USE WITH 10 CM GEL BOX, 2.0 MM THICKNESS

HGB10-25-2 1/pk
EUR 70.8
Description: Lab Equipment; Axygen Branded EQ

12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-03 Ea
EUR 153.6

12 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-04 Ea
EUR 153.6

12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-05 Ea
EUR 153.6

16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-06 Ea
EUR 153.6

16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-07 Ea
EUR 153.6

16 Well Comb ,2mm Thick For Enduro 10cm Gel System

LE1000C10-08 Ea
EUR 153.6

25 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-11 Ea
EUR 153.6

15 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-03 Ea
EUR 157.2

14 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-04 Ea
EUR 157.2

16 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-05 Ea
EUR 157.2

20 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-11 Ea
EUR 157.2

20 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-12 Ea
EUR 157.2

20 Well Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-13 Ea
EUR 157.2

16 Well Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-01 Ea
EUR 165.6

25 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-05 Ea
EUR 165.6

30 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-06 Ea
EUR 165.6

16 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-05 Ea
EUR 148.8

16 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-06 Ea
EUR 148.8

5 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-07 Ea
EUR 148.8

0.5-10UL 8-CHANNEL, DIGITAL PIPETTOR

AP-8-10 1/pk
EUR 510
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6453.54

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

comb 10 sample, 0.8mm

EVS3100-C10-0.8 ea
EUR 70.8

comb 10 sample, 1.5mm

EVS3100-C10-1.5 ea
EUR 70.8

comb 10 sample, 0.8mm

EVS3200-C10-0.8 ea
EUR 78

comb 10 sample, 1.5mm

EVS3200-C10-1.5 ea
EUR 78

comb 10 sample, 0.8mm

EVS3300-C10-0.8 ea
EUR 78

comb 10 sample, 1.5mm

EVS3300-C10-1.5 ea
EUR 78

10-well comb, 1mm

2398207 3unit
EUR 303.6

Gel Tray 10 X 10 Cm

LE1000GT-03 Ea
EUR 170.4

comb 8 sample, 0.8mm

EVS3100-C8-0.8 ea
EUR 70.8

comb 8 sample, 1.5mm

EVS3100-C8-1.5 ea
EUR 70.8

1730 8 SNAP-SEAL 8 OZ

1730-8 100/pk
EUR 88.8
Description: Disposable Plastic; Plastic Containers

20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-09 Ea
EUR 153.6

16 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-06 Ea
EUR 157.2

16 Well Mc Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-07 Ea
EUR 157.2

18 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-08 Ea
EUR 157.2

18 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-09 Ea
EUR 157.2

28 Well Mc Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-14 Ea
EUR 157.2

28 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-15 Ea
EUR 157.2

30 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-16 Ea
EUR 157.2

20 Well Mc Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-02 Ea
EUR 165.6

20 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-03 Ea
EUR 165.6

20 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-04 Ea
EUR 165.6

40 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-07 Ea
EUR 165.6

40 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-08 Ea
EUR 165.6

40 Well Mc Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-09 Ea
EUR 165.6

12 Well Mc Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-03 Ea
EUR 148.8

12 Well Mc Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-04 Ea
EUR 148.8

gel casting base 10 cm

EVS1100-BASE ea
EUR 180

comb 10 sample, 1.0 mm

EHS3100-C10-1.0 ea
EUR 87.6

comb 10 sample, 1.5 mm

EHS3100-C10-1.5 ea
EUR 87.6

comb 10 sample, 1.0 mm

EHS3200-C10-1.0 ea
EUR 78

comb 10 sample, 1.5 mm

EHS3200-C10-1.5 ea
EUR 78

comb 10 sample, 1.0 mm

EHS3400-C10-1.0 ea
EUR 87.6

comb 10 sample, 1.5 mm

EHS3400-C10-1.5 ea
EUR 87.6

Comb 10 sample 2mm thick

MS1010MC2 EACH
EUR 45.6

Comb 10 sample 0.75mm thick

MS1510075 EACH
EUR 50.16

Comb 10 sample 1mm thick

MS15101 EACH
EUR 52.44

Comb 10 sample 1.5mm thick

MS151015 EACH
EUR 50.16

Comb 10 sample 2mm thick

MS15102 EACH
EUR 52.44

Comb 10 sample 0.75mm thick

MS2010075 EACH
EUR 54.72

Comb 10 sample 1mm thick

MS20101 EACH
EUR 57

Comb 10 sample 1.5mm thick

MS201015 EACH
EUR 54.72

Comb 10 sample 2mm thick

MS20102 EACH
EUR 57

Comb 10 sample 0.75mm thick

MS710075 EACH
EUR 36.48

Comb 10 sample 1mm thick

MS7101 EACH
EUR 38.76

Comb 10 sample 1.5mm thick

MS71015 EACH
EUR 36.48

Comb 10 sample 2mm thick

MS7102 EACH
EUR 38.76

Comb 10 sample 0.75mm thick

VS1010075 EACH
EUR 36.48

Comb 10 sample 1mm thick

VS10101 EACH
EUR 38.76

Comb 10 sample 1.5mm thick

VS101015 EACH
EUR 35.34

Comb 10 sample 2mm thick

VS10102 EACH
EUR 38.76

Comb 10 sample 0.75mm thick

VS2010075 EACH
EUR 50.16

Comb 10 sample 1mm thick

VS20101 EACH
EUR 53.58

Comb 10 sample 1.5mm thick

VS201015 EACH
EUR 50.16

Comb 10 sample 2mm thick

VS20102 EACH
EUR 53.58

S 10 N-8 G-ST Dispersing element

HOM2488 EACH
EUR 1772.7

comb 8 sample, 1.0 mm

EHS3100-C8-1.0 ea
EUR 87.6

comb 8 sample, 1.5 mm

EHS3100-C8-1.5 ea
EUR 87.6

comb 8 sample, 1.0 mm

EHS3200-C8-1.0 ea
EUR 78

comb 8 sample, 1.5 mm

EHS3200-C8-1.5 ea
EUR 78

comb 8 sample, 1.0 mm

EHS3300-C8-1.0 ea
EUR 78

comb 8 sample, 1.5 mm

EHS3300-C8-1.5 ea
EUR 78

Comb 8 sample 0.75mm thick

MS108075 EACH
EUR 43.32

Comb 8 sample 1mm thick

MS1081 EACH
EUR 45.6

Comb 8 sample 1.5mm thick

MS10815 EACH
EUR 43.32

Comb 8 sample 2mm thick

MS1082 EACH
EUR 45.6

Comb 8 sample 0.75mm thick

MS78075 EACH
EUR 36.48

Comb 8 sample 1mm thick

MS781 EACH
EUR 38.76

Comb 8 sample 1.5mm thick

MS7815 EACH
EUR 36.48

Comb 8 sample 2mm thick

MS782 EACH
EUR 38.76

Comb 8 sample 1mm thick

FMMS81 EACH
EUR 33.06

Comb 8 sample 1.5mm thick

FMMS815 EACH
EUR 33.06

AXYGEN® 20-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-20MC-1 1/pk
EUR 70.8
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 20-WELL MULTI-CHANNEL COMPATIBLE COMB FOR USE WITH 10 CM GEL BOX, 1.5 MM THICKNESS

HGB10-20MC-15 1/pk
EUR 70.8
Description: Lab Equipment; Axygen Branded EQ

Individual Reaction Mix 8

G065-8 200 reactions
EUR 200.4

SU14813 double bond Z

A3845-10 10 mg
EUR 602.4
Description: SU14813 is an oral, multitargeted tyrosine kinase inhibitor (TKI) targeting vascular endothelial growth factor receptors (VEGFR), platelet-derived growth factor receptors (PDGFR), Kit, and fms-like tyrosine kinase 3 (FLT-3).

(S)-10-Hydroxycamptothecin

B4745-100 100 mg
EUR 177.6
Description: IC50: 0.31 ?M10-Hydroxycamptothecin is a dose-dependent growth inhibitor of human microvascular endothelial cells (HMEC), and significantly inhibits the migration of HMEC with IC50 of 0.63 ?M, resulting in a dose-dependent inhibition of tube formation with IC50 of 0.96 ?M [1,2].

(S)-10-Hydroxycamptothecin

B4745-25 25 mg
EUR 115.2
Description: IC50: 0.31 ?M10-Hydroxycamptothecin is a dose-dependent growth inhibitor of human microvascular endothelial cells (HMEC), and significantly inhibits the migration of HMEC with IC50 of 0.63 ?M, resulting in a dose-dependent inhibition of tube formation with IC50 of 0.96 ?M [1,2].

(S)-10-Hydroxycamptothecin

B4745-5.1 10 mM (in 1mL DMSO)
EUR 142.8
Description: IC50: 0.31 ?M10-Hydroxycamptothecin is a dose-dependent growth inhibitor of human microvascular endothelial cells (HMEC), and significantly inhibits the migration of HMEC with IC50 of 0.63 ?M, resulting in a dose-dependent inhibition of tube formation with IC50 of 0.96 ?M [1,2].

(S)-10-Hydroxycamptothecin

HY-N0095 100mg
EUR 207.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

Human IL-8 Recombinant Protein

R00423-8 5ug/vial
EUR 310.8
Description: Interleukin-8 (IL-8), also known as CXCL8, is an ELR-positive CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. Human IL-8 Recombinant Protein is purified interleukin-8 produced in yeast.

BRAND Transferpette S multi-8 10-100ul adjust vol

PIP5237 EACH
EUR 734.39

Gel Tray 7 X 10 Cm

LE1000GT-02 Ea
EUR 160.8

Gel Tray 15 X 10 Cm

LE1000GT-04 Ea
EUR 181.2

PAGE Gel Transfer Buffer, 10 x

GR103018 1 L
EUR 94.8

T-Pro EZ Gel Solution 10%

JB02-B010M 500ml/BT
EUR 266.4

T-Pro EZ Gel Solution 10%

JB02-B010S 100ml/BT
EUR 162

SYBR Safe DNA Gel Stain

A8743-10 10x1 ml
EUR 838.8

Hi-Bind? T-Gel-Agarose

M1313-10
EUR 261.6

Sprint™ 8 Clinical Centrifuge with 8 x 15ml fixed angle rotor, 115V

C5000-8 1 each
EUR 537.8

comb 1 mm thick 10 sample

EVS1100-C10-1.0 ea
EUR 50.4

comb 1.5 mm thick 10 sample

EVS1100-C10-1.5 ea
EUR 50.4

comb 1 mm thick 10 sample

EVS1300-C10-1.0 ea
EUR 52.8

comb 1.5 mm thick 10 sample

EVS1300-C10-1.5 ea
EUR 52.8

comb 1 mm thick 10 sample

EHS1100-C10-1.0 ea
EUR 48

comb 1.5 mm thick 10 sample

EHS1100-C10-1.5 ea
EUR 48

comb 1 mm thick 10 sample

EHS1300-C10-1.0 ea
EUR 57.6

comb 1.5 mm thick 10 sample

EHS1300-C10-1.5 ea
EUR 57.6

comb 1 mm thick 10 sample

EHS1400-C10-1.0 ea
EUR 67.2

comb 1.5 mm thick 10 sample

EHS1400-C10-1.5 ea
EUR 67.2

1 mm 10-well Comb, RM

2328948 3unit
EUR 345.6

Comb 10 sample MC 1mm thick

MS1010MC1 EACH
EUR 45.6


Conclusion: Although the WGS-based typing has logically become the new reference standard, we show here that the PFGE can be used with confidence to local outbreak investigations caused by P. aeruginosa.

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