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Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation

Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Posted by Troy

A recent study found that the method of agarose gel electrophoresis (AGE) produced two great bands that differ according to the isoenzyme ALP liver and bone (ALP2 and ALP3, respec-tively) in the serum of bovine treated with protease and neuraminidase (PN-treatment), although there fears that the intestinal ALP isoenzymes (ALP5) often overlap with ALP3 in human serum treated with neuraminidase. Because ALP5 separated from ALP3 in serum, bovine treated with protease alone (P-treatment), we use the method is modified using both P and PN-treated cattle sera to measure the activity of three isoenzymes of ALP in 53 cows Lacta-ting Holstein: 24 primiparous and 29 multipara.

After electrophoresis, 51 of 53 samples (96.2%) had a P-treatment produces a fraction that differ according to the ALP5, as did the control serum. All sera PN-treated produce ALP2 definite fraction. ALP3 fraction is calculated as the remainder after excluding ALP2 and ALP5. Total ALP activity (t-ALP) and ALP3 in primiparous cows higher than those in multiparous cows (p ⟨0,001) in the early-to-peak [10-110 days in milk (DIM)] and mid (111-220 DIM) lactation.

In a multi-parous cows, ALP3 activity in late lactation (221-477 DIM) was significantly higher than in the early-to-peak lactation. Thus, AGE-modified method described here is capable of discrimi-nate the three factions of the ALP isoenzymes in sera lactating cows. ALP isoenzyme patterns circu AGE-Lating will contribute to understanding the physiological status of bone metabolism in lactating cows. Vaccines against infectious diseases is needed.

Therefore, the development of modern analytical methods must be as efficient as possible to accelerate the development of vaccines. The purpose of this study was to identify the critical parameter method (CMPS) and to establish a set of measures to efficiently develop and validate the CE-SDS method for protein analysis based buffer gel vaccine commercially available. The CMPS gained from reviewing the literature and tested the effects of dilution buffer gel.

Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation
Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation

Comparison of PFGE and confirms typing-based whole genome sequencing accuracy of pulsed-field gel electrophoresis to investigate an outbreak of Pseudomonas aeruginosa local

Objective: To determine whether pulsed-field gel electrophoresis (PFGE) accurately recognizes isolates belonging to the cluster is defined by a technique based on whole genome sequencing (WGS) using Pseudomonas aeruginosa as a model.


Methods: We selected 65 ST395 isolates of P. aeruginosa was isolated in seven European hospitals between 1998 and 2012. The isolates typed by PFGE and sorted by WGS. An analysis of the genome core multi-locus sequence typing (cgMLST) by 3831 genes carried by a homemade pipe.
Findings: PFGE identified eight pulsotypes and cgMLST distinguished nine and nine single cluster. cluster of five cgMLST and pulsotypes (31/65 isolates) coincide perfectly.

Isolates without apparent epidemiological links grouped by PFGE separated by cgMLST (16/65 isolates) distinguishes the city, indicates that PFGE should be saved to a local outbreak investigation. Importantly, they share the isolates hypermutator pulsotype with their parents (16/65 isolates), while they are not recognized by cgMLST. This suggests that PFGE is less affected than typing based WGS by accelerated genetic drift occurs in epidemic P. aeruginosa.

Agarose Gel Electrophoresis Kit

MB570-10PR 1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 17.3

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 23.23

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 35.42

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 62.99

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

HiPer® Agarose Gel Electrophoresis Teach

HTBM001-10PR 1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach

WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell

HTES1013 set
EUR 602

WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell

HTES1014 set
EUR 448

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

Aminoacetic acid, ?For electrophoresis

GRM2233-100G 1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis

Aminoacetic acid, ?For electrophoresis

GRM2233-500G 1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis

Electrophoresis Buffer (10×)

E-BC-R331-100mL 100mL
EUR 15

Electrophoresis Buffer (10×)

E-BC-R331-200mL 200mL
EUR 20

Electrophoresis Buffer (10×)

E-BC-R331-500mL 500mL
EUR 25

Electrophoresis Buffer (10×)

E-BC-R331-each each Ask for price

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Agarose, medium EEO ?For electrophoresis

RM6249-100G 1 unit
EUR 119.06
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-10G 1 unit
EUR 19.63
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-1KG 1 unit
EUR 1089.43
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-25G 1 unit
EUR 36.87
Description: Agarose, medium EEO ?For electrophoresis

Chiller for Electrophoresis systems - EACH

CSLCHILLER EACH
EUR 7642.35

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

Electrophoresis baffle for Life mini tank

HTDB03 pcs
EUR 5.6

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 74.66

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 139.51

Electrophoresis baffle for Life Technology Novex

HTDB02 pcs
EUR 2.8

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

TEMED, electrophoresis grade

RM10358-100G 1 unit
EUR 301.18
Description: TEMED, electrophoresis grade

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

electrophoresis power supply

EV245 ea
EUR 574.8

Electrophoresis Power Supply

LA690-1NO 1 unit
EUR 432.84
Description: Electrophoresis Power Supply

ComboMiniTM Horizontal Electrophoresis System (110V)

CBMN-7 each
EUR 500

ComboMiniTM Horizontal Electrophoresis System (220V)

CBMN-8 each
EUR 530

Electrophoresis baffle for Bio-Rad Mini-PROTEAN

HTDB01 pcs
EUR 2.1

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

10X MOPS Electrophoresis Buffer

ML050-500ML 1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster)

HTES2001 set
EUR 322

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

myGel Mini Electrophoresis System

E1101 1 each
EUR 636.38

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

miniONE Electrophoresis System - EACH

ELE3400 EACH
EUR 777.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 651.6

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

Alkaline Hemoglobin Electrophoresis Kit

MBP001-112RUNS 1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP001-224RUNS 1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP008-224RUNS 1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Paper Electrophoresis Kit (Cell

MBP009-20RUNS 1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell

WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

LA1070-1NO 1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

Electrophoresis Power Supply (2 termina

LA842-1NO 1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

Cellulose Acetate Electrophoresis System

LA1027-1NO 1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System

WSHT-Basic Electrophoresis Power Supply

HTES1001 set
EUR 557.2

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Hi-Eco Mini Horizontal Electrophoresis K

LA851-1NO 1 unit
EUR 205.56
Description: Hi-Eco Mini Horizontal Electrophoresis K

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

WSHT-Universal Electrophoresis Power Supply

HTES1003 set
EUR 784

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

WSHT-miniPRO4 Mini Vertical Electrophoresis Cell

HTES1009 set
EUR 795.2

WSHT-miniPRO2 Mini Vertical Electrophoresis Cell

HTES1010 set
EUR 516.6

WSHT-easyPRO4 Mini Vertical Electrophoresis Cell

HTES1011 set
EUR 837.2

WSHT-easyPRO2 Mini Vertical Electrophoresis Cell

HTES1012 set
EUR 544.6

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

WSHT-miniDNA Mini Horizontal Electrophoresis Cell

HTES1008 set
EUR 193.2

Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH

ELE1111 EACH
EUR 961.2

WSHT-Multi-Functional Electrophoresis Power Supply

HTES1004 set
EUR 896

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell

HTES2001-P set
EUR 459.2

WSHT-liteDNA Universal Horizontal Electrophoresis Cell

HTES1007 set
EUR 259

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 665.55

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

WSHT-midiDNA Multipurpose Horizontal Electrophoresis Cell

HTES1006 set
EUR 278.6

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa)

MKPZ010-200mL 200μL
EUR 72

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 727.06

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 757.35

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH

VS10WCES EACH
EUR 1614.21

Green combination double junction pH electrode gel with epoxy body

GD9106BNWP EACH
EUR 226.86

Double Junction combination pH electrode with epoxy body low maintenance gel - EACH

9156DJWP EACH
EUR 324

Double Junction combination pH electrode - EACH

9102DJWP EACH
EUR 279.45

Double Comb for miniONE - EACH

ELE3404 EACH
EUR 41.85

double silver electrode, s7

SP05X ea
EUR 279.6

double platinum electrode, s7

SP04X ea
EUR 178.8

DOUBLE SILVER ELECTRODE, PEEK, S7

SP06X each
EUR 310

Green combination double junction refillable pH electrode with epoxy body

GD9156BNWP EACH
EUR 250.8

VCV VERTICAL ELECTROPHORSIS

IB62000 SYSTEM
EUR 691.24

Electrode Filling Sol Double Junction 10% w/v KNO3 - 100ML

EFSKNO 100ML
EUR 37.8

Double Junction combination pH electrode with glass body semi-micro tip - EACH

9110DJWP EACH
EUR 399.6

Electrode Filling Sol Double Junction 0.1M (NH4)2SO4 - 100ML

EFS01AS 100ML
EUR 51.26

Electrode Filling Sol Double Junction 2M (NH4)2SO4 - 250ML

EFS2-250ML 250ML
EUR 76.38

Electrode Filling Sol Double Junction 2M (NH4)2SO4 - 100ML

EFS2AS 100ML
EUR 40.5

DOUBLE SLAB VERTICAL GEL SYST.

JVD-80 -
EUR 933.21

Inlab Electrode pH Dairy Comb - EACH

59904591 EACH
EUR 449.55

SLS pH Electrode Comb AgCl Spear BNC - EACH

PHE4126 EACH
EUR 292.29

Gel electrode Hanna - EACH

HI-12170 EACH
EUR 248.4

Combination pH electrode with epoxy body low maintenance gel - EACH

9146BN EACH
EUR 130.95

Gel Filled Ph Electrode - EACH

PHE4090 EACH
EUR 225.45

20X ELECTROPHAST BUFFER

IB70190 50ML
EUR 11.93

20X ELECTROPHAST BUFFER

IB70191 1L
EUR 35.22

STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

Gel pH Electrode + Din Connector - EACH

PHM4121 EACH
EUR 260.55

Hach 5010T pH electrode Gel With Temperature Electrode - EACH

WAT4594 EACH
EUR 427.95

SLS Solid Gel pH Electrode BNC - EACH

PHE1120 EACH
EUR 101.35

Mettler pH Electrode Gel Five Easy - EACH

PHM4508 EACH
EUR 120.15

SS grid electropolished for size 30 - EACH

INC8202 EACH
EUR 149.85

SS grid electropolished for HPP750 - EACH

CHA4054 EACH
EUR 357.75

8 Well Comb ,1mm Thick For Enduro 7cm Gel System

LE1000C7-09 Ea
EUR 148.8

8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
EUR 153.6

8 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-17 Ea
EUR 157.2

8 Well Comb , 0.75mm Thick For Enduro 7cm Gel System

LE1000C7-08 Ea
EUR 148.8

SS grid electropolished for sizes 260/400 - EACH

CHA4052 EACH
EUR 252.45

Plant Electroporation Buffer for Transfection

GR103051 1 L
EUR 119

Swabs Double for Protein Residue

MIC6760 PK50
EUR 87.78

AXYGEN® 8-WELL COMB FOR USE WITH 10 CM GEL BOX, 1.0 MM THICKNESS

HGB10-8-1 1/pk
EUR 70.8
Description: Lab Equipment; Axygen Branded EQ

Gill’s Hematoxylin #2, double strength for Histology & Cytology

24243-1000 1000ml
EUR 108
Description: 7732-18-5

Gill’s Hematoxylin #2, double strength for Histology & Cytology

24243-500 500ml
EUR 65
Description: 7732-18-5

10 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-01 Ea
EUR 148.8

10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-02 Ea
EUR 148.8

Extra casting set for 10.5x10cm and 10.5x6cm gels. Includes stand, trays, E1101-COMB1 double-sided combs

E1200-CS1 1 each
EUR 48.7

10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
EUR 157.2


Conclusion: Although the WGS-based typing has logically become the new reference standard, we show here that the PFGE can be used with confidence to local outbreak investigations caused by P. aeruginosa.

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