Genetic-based approaches-including mutant screens population, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. Nonetheless, genetic tools are not without limitations. One alternative approach to studying meiosis plants, especially in polyploidy as Triticum aestivum L. (wheat bread), is proteomics. However, the proteins using proteomics-based approach is rarely depicted, with only two early learning efforts reported plant meiosis.
Here, we report the initial investigation meiosis wheat bread using proteomics. Five places differently expressed proteins were identified using 2D gel electrophoresis (2DGE) in the protein extracts of the four stages of meiosis were collected and three genotypes (wild-type Chinese Spring, ph1b and ph2a wheat mutant lines). mass spectrometry (MS / MS) identification tandem peptides from this protein led to the isolation and characterization of full-length clones of wheat Speckle-type POZ protein, a SF21-like protein and HSP70, and partial coding sequence of a hexose transporter.
Significantly, the function is suspected of Speckle-type POZ protein and HSP70 was confirmed using in vitro DNA binding test. Through the use of 2DGE proteomics approach, we show that proteomics is an alternative to the genetic-based approach when studying meiosis in wheat. More importantly, we reported a potential role for Speckle-type POZ protein and HSP70 in a chromosome pair during the early stages of meiosis in bread wheat.
Thus, 14 and 7 potential glycan structures were successfully solved, respectively. In total, 15 and 22 potential glycan composition glycan structures identified and marked for AZGP1, including several different structures with the same composition. In particular, five potential glycan structures identified as a unique signature of lung cancer. Our advanced strategy holds promise for a thorough identification of glycan structures on the glycoprotein target biomarker.
Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Rate Securities genotoxic pendimetalin in Chinese Hamster Cells During the Single Cell Gel Electrophoresis (Comet) Assay
Objective: pendimetalin (N- (1-ethylpropyl) -3.4-dimethyl-2,6-dinitrobenzeneamine) is a compound that selectively dinitroaniline herbicide weed control. This is a cell division and growth inhibitors. It’s down the plant in a short time after the seedlings. It is a land and water pollutants due to the widespread use of the formulation in Turkey and around the world.
Pendimetalin manufactured and imported by Turkey. Pendimetalin is slightly toxic substances classified in toxicity class 3 by the United States Environmental Protection Agency (USEPA). Although classified as a Group C (possible human carcinogen) compounds by USEPA, there are a number of studies on its genotoxic effects. The purpose of this study was to evaluate the in vitro genotoxic effects of different concentrations of pendimetalin in more Chinese hamster (CHO) cells by single cell gel electrophoresis (comet) assay.
Materials and Methods: Cells were incubated with 1, 10, 100, 1000 and 10000 pM concentration of pendimetalin for 30 min at 37 ° C and DNA damage compared to CHO cells treated with pendimetalin. 50 pM hydrogen peroxide is used as a positive control.
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Results: No significant cytotoxic effects were observed in the studied concentration range. DNA damage in CHO cells increased significantly in pendimetalin concentrations of 1, 100, 1000 and 10000 pM, however, a significant reduction was observed in 10 pM pendimetalin concentration. Conclusions: Our results indicate that the concentrations 1-10000 pM of pendimetalin induces DNA damage in CHO cells, as assessed by the comet assay.
