Antibodies, Assay Kits, Biology Cells, cDNA, Clia Kits, Culture Cells, Devices, DNA, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Recombinant Proteins, Ria Kits, RNA, Test Kits, Vector & Virus, Western Blot

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Posted by Troy

Genetic-based approaches-including mutant screens population, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. Nonetheless, genetic tools are not without limitations. One alternative approach to studying meiosis plants, especially in polyploidy as Triticum aestivum L. (wheat bread), is proteomics. However, the proteins using proteomics-based approach is rarely depicted, with only two early learning efforts reported plant meiosis.

Here, we report the initial investigation meiosis wheat bread using proteomics. Five places differently expressed proteins were identified using 2D gel electrophoresis (2DGE) in the protein extracts of the four stages of meiosis were collected and three genotypes (wild-type Chinese Spring, ph1b and ph2a wheat mutant lines). mass spectrometry (MS / MS) identification tandem peptides from this protein led to the isolation and characterization of full-length clones of wheat Speckle-type POZ protein, a SF21-like protein and HSP70, and partial coding sequence of a hexose transporter.

Significantly, the function is suspected of Speckle-type POZ protein and HSP70 was confirmed using in vitro DNA binding test. Through the use of 2DGE proteomics approach, we show that proteomics is an alternative to the genetic-based approach when studying meiosis in wheat. More importantly, we reported a potential role for Speckle-type POZ protein and HSP70 in a chromosome pair during the early stages of meiosis in bread wheat.

Thus, 14 and 7 potential glycan structures were successfully solved, respectively. In total, 15 and 22 potential glycan composition glycan structures identified and marked for AZGP1, including several different structures with the same composition. In particular, five potential glycan structures identified as a unique signature of lung cancer. Our advanced strategy holds promise for a thorough identification of glycan structures on the glycoprotein target biomarker.

Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics

Rate Securities genotoxic pendimetalin in Chinese Hamster Cells During the Single Cell Gel Electrophoresis (Comet) Assay

Objective: pendimetalin (N- (1-ethylpropyl) -3.4-dimethyl-2,6-dinitrobenzeneamine) is a compound that selectively dinitroaniline herbicide weed control. This is a cell division and growth inhibitors. It’s down the plant in a short time after the seedlings. It is a land and water pollutants due to the widespread use of the formulation in Turkey and around the world.

Pendimetalin manufactured and imported by Turkey. Pendimetalin is slightly toxic substances classified in toxicity class 3 by the United States Environmental Protection Agency (USEPA). Although classified as a Group C (possible human carcinogen) compounds by USEPA, there are a number of studies on its genotoxic effects. The purpose of this study was to evaluate the in vitro genotoxic effects of different concentrations of pendimetalin in more Chinese hamster (CHO) cells by single cell gel electrophoresis (comet) assay.


Materials and Methods: Cells were incubated with 1, 10, 100, 1000 and 10000 pM concentration of pendimetalin for 30 min at 37 ° C and DNA damage compared to CHO cells treated with pendimetalin. 50 pM hydrogen peroxide is used as a positive control.

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 17.3

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 23.23

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 35.42

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 62.99

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6793.2

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 74.66

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 139.51

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

electrophoresis power supply

EV245 ea
EUR 574.8

miniONE Electrophoresis System

ELE3400 EACH
EUR 691.2

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

myGel Mini Electrophoresis System

E1101 1 each
EUR 623.9

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1602

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 651.6

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 854.4

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 712.8

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1434.85

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

22 TOOTH COMB, 1.5MM

IB52122 CUSTOM COMB
EUR 129.36

COMB, HR2025, 2MM 12 TOOTH

IB56115 -
EUR 144.09

QSV COMB 1.5MM X 12 TOOTH

IB59050 -
EUR 110.27

GD-710 COMB 1.5MM,8 TOOTH

IB51155 -
EUR 126.43

MP COMB, 2MM 10 AND 16 TOOTH

IB52075 -
EUR 129.36

VCV VERTICAL ELECTROPHORSIS

IB62000 SYSTEM
EUR 691.24

JSB-75 COMB - .8MM X 18 TOOTH

CB75-0818 -
EUR 72.02

Inlab Electrode pH Dairy Comb

59904591 EACH
EUR 399.6

Gel electrode Hanna

HI-12170 EACH
EUR 220.8

SLS pH Electrode Comb AgCl Spear BNC

PHE4126 EACH
EUR 259.81

Combination pH electrode with epoxy body low maintenance gel

9146BN EACH
EUR 116.4

Gel Filled Ph Electrode

PHE4090 EACH
EUR 200.4

Green combination double junction pH electrode gel with epoxy body

GD9106BNWP EACH
EUR 226.86

Gel pH Electrode + Din Connector

PHM4121 EACH
EUR 231.6

SLS Solid Gel pH Electrode BNC

PHE1120 EACH
EUR 90.09

Hach 5010T pH electrode Gel With Temperature Electrode

WAT4594 EACH
EUR 380.4

Double Junction combination pH electrode with epoxy body low maintenance gel

9156DJWP EACH
EUR 288

20X ELECTROPHAST BUFFER

IB70190 50ML
EUR 11.93

20X ELECTROPHAST BUFFER

IB70191 1L
EUR 35.22

Mettler pH Electrode Gel Five Easy

PHM4508 EACH
EUR 106.8

Optional generator 14mmx130mm saw tooth for 50-250mL vessels

HOM3068 EACH
EUR 1560

SS grid electropolished for size 30

INC8202 EACH
EUR 133.2

SS grid electropolished for HPP750

CHA4054 EACH
EUR 318

Optional generator 10mmx115mm saw tooth for 15mL 50mL tubes

HOM3066 EACH
EUR 525.6

SS grid electropolished for sizes 260/400

CHA4052 EACH
EUR 224.4

Optional generator, 10mm x 115mm saw tooth, for 15ml & 50ml tubes

D1000-M10 1 PC
EUR 399.2

Plant Electroporation Buffer for Transfection

GR103051 1 L
EUR 119

Optional generator, 10mm x 115mm saw tooth, for 15ml and 50ml tubes

BCM1235 1 pcs, 1 UNIT
EUR 517.18

Replacement generator pack, 7mm x 50mm saw tooth for microtubes, pack of 5

D1000-M7 1 PC
EUR 711.1

Optional generator 14mm x 130mm saw tooth, for 50ml tubes up to 250ml vessels

BCM1236 1 pcs, 1 UNIT
EUR 1407.54

Optional generator 14mm x 130mm saw tooth, for 50ml tubes up to 250ml vessels

D1000-M14 1 PC
EUR 1193.3

SLS Electrode Stand for 4 Electrodes

PHM4045 EACH
EUR 167.12

High temperature glass bodied combination electrode for solutions up to 110°C

924079 EACH
EUR 293.76

Ar.1193 Electroporation Competent Cell

ACC-126 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.1193 Electroporation Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

Ar.A4 Electroporation Competent Cell

ACC-122 10 tubes, 20 tubes, 50 tubes, Ask for price
Description: Applications: Ar.A4 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

electrolyte for gal.o2/zn electr., 500ml

B550 ea
EUR 57.6

membranes+electrolyte for o2/pb electr

SZ02K ea
EUR 165.6


Results: No significant cytotoxic effects were observed in the studied concentration range. DNA damage in CHO cells increased significantly in pendimetalin concentrations of 1, 100, 1000 and 10000 pM, however, a significant reduction was observed in 10 pM pendimetalin concentration.
Conclusions: Our results indicate that the concentrations 1-10000 pM of pendimetalin induces DNA damage in CHO cells, as assessed by the comet assay.

Related Post

Leave A Comment